"ARRKG -- 137/ ANALYTICAL METHOD VALIDATION FOR THE DETERMINATION OF PLANT TISSUES PERFLUOROOCTANESULFONATE, POTASSIUM SALT (PFOS) IN `WILDLIFE INTERNATIONAL, LTD.

: mteratice Correspondence S00 t C:iHe .E.

caerinOf Ip wSeY e siaF00E0 HO-020A0R-16263-703545 RECEIVED OPPT ceIC WONAR 20 Aif11: 43 March 17, 2000 (DAotctun:meSnetctPiroonce8s(s)inCgooCrednitnearto(r7)407) obPT Room G-099 US EPA 401 M Street, SW.

; . 2M Speci Marit StPChSoLMreN 55144-1000 AR Saniitized 5Co1p9y 3 3M May 28, 1999 opps bark 000 1176gw Frank D.

CAS Number 307-35-7 3M Company AR 226 - C 6 Voluntary Use and Exposure Information Profile Perfluorooctanesulfonyl fluoride (POSF) L mn \ II.

AR 226 . 0689 BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSn,g pFeCr-f9iu5o,roorocatsanaescuolmfopnoanteen,twhoifcFhC-m2a0y1.al(s1o-be Octanesulfonic acid, (CAS #2795-39-3) perfiuoroalkyl sulfonate salts (5) + (5140F)) Remarks: is FC-201.

AR 286 - 070] BIODEGRADATION (BOD/COD) EST SUBSTANCE Identity: MriexfetrurreedctoontaasinPiFnOgSp,erFfCl-u9o5r,ooocrtaasneasucfoomnpatoenewnhticohf FmCa-y20a1lsFo.be (1-Octanesulfonic acid, 1,1,2,2.3,3.44,55.66.7,7.88,8- heptadecafluoro-, potassium salt, CAS # 2795-39-3) Rsaemmpalreksis: FC-T2h01eF3.MCpurrordeuncttiionfnolromtantiuomnbienrdiwcaastesnotthantotFeCd-.20T1hFe itsesat m8i.x2t5u%reaocfr3yl.ic0%foaPmFeOrS,,7.306%.0s%oddiieutmhyolcteynle sgullyfcaotle,bu6t.yl0%etshuelrf,o3n6at.e6d%awlkaytler, ether sodium salts, 3.0% triethanolamine, and 0.15% tolyl triazole.

#826 - 0711 BIODEGRADATION (301E) JESTSUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfopnoanteen,twhoficFhC-m2a0y3.al(s1o-be Octanesulfonic acid) (CAS # 2795-393).

ARRRG - O04 ACUTE TOXICITY TO FISH (RAINBOW TROUT) TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f8l5u,oroorocatsanaescuolmfponoanteen,twhoficFhC-m3a0y52a.ls(o1b-e Octanesulfonic acid) (CAS # 2795-39-3).

bn Te wt oe an al } `DuPont HaskellLaboratory Disecior,Applied Tox . benTuxicology DuPoat Haskell Laboratory v P.0.Box 50 Elkton Road Newark,Delaware (9714-0050 : Phioge: (302) 366-5259 OS a v Hai kae aa rr ocr 40 220-1559 * Dumcas Control Office (7407) `Room G99 East Tower ATTN: FYT 4OUf0Sf1.icMEevoSftirrPeoeotln,lumSteiWon.ntPPrreovteencttiioonnaAngdeTnocxyics `Washington, D.C. 20460-0001 .

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TTT a Specialy Materials 3SuMPCaeunlteMrN 55144-1000 S17 0 Ae226-1717 May 11,2004 TSCA Non-Confidential Information Center (7407T) Office of Pollution Prevention and Toxics (OPPT) EDoPcAkeWteAstR-B2u2il6ding, Room B-146 1301 Constitution Ave., N.W.

BACK TO MAIN Executive Summary of Hydrolysis Studies 3M has completed a series of studies of the hydrolytic reactions of various fluorochemicals.

STABILITY IN WATER (PHOTOLYSIS) TEST SUBSTANCE Identity: Perfluorooctanesulfonate-potassium salt.

Estrous Cycling and Natural Delivery/Litter Observation Data F1 Estrous Cycling Data, From Data Analysis Presented at EPA on August 30, 2002 a APFO Dose Group (mg/kg/day) 0 1 3 10 30 All 4-Day Cycles 3-5 Day Cycles 14/30 24/30 19/29 26/29 9/30 21/30 15/29 25/29 15/30 27/30 Cycle Length (Days) 2 3 4 5 6 7 8 Mean Cycle Length (Days) S.D. 1 2 75 18 5 1 2 4.3 + 0.8 Incidence of Cycle Length Per Group 0 1 80 16 2 1 0 4.2 0.5 5 7 57 32 5 0 1 4.3 + 0.9 2 5 84 14 3 0 0 4.1 0.6 5 5 86 17 0 0 2 4.1 0.8 Aberrant Cycles b Parental > 4 Days Diest. 2 1 1 4 1 > 3 Days Est. 17 13 15 16 13 F1 > 4 Days Diest. 5 3 3 5 3 > 3 Days Est. 25 25 29 11 14 Distribution of Cycle Status at Start of 21-Day Evaluation Period, F1 P 1 4 1 2 5 E 8 10 11 14 11 M 6 2 2 0 2 D 15 13 16 13 12 Distribution Rats in Estrous at End of 21-Day Evaluation Period, F1 End in Estrous 13 17 18 19 19 Mean Implantations, Liveborn, and Stillborn S.D. per Litter by Group" Parental Litters Implantations Livebom StiUbom 15.3 1.9 14.2 _: 2.2 0.1 0.3 15.6 2.1 14.2 + 2.6 15.2 2.3 13.4 3.6 0.4 1.4 0.2 0.4 F1 Litters 15.6 + 2.0 13.8 3.0 0.1 0.4 15.3 2.0 13.4 3.3 0.2 0.6 Implantations Livebom Stillborn 14.8 3.0 13.6 + 2.9 0.2 0.5 15.8 2.1 13.7 2.4 0.2 0.5 14.7 2.8 13.8 3.0 0.2 0.6 15.0 2.9 13.5 3.1 0.2 0.5 14.5 2.9 13.3 2.8 0.1 0.2 a Original analysis counted cycles from the day following estrous (or proestrous if diestrus followed proestrous without observation of estrous) to the day following the end of the next estrous.