Document 6R1rva0b9ZKx8aeG30BkXG1pg

FOR DU FONT USE ONLY Copies to: AR226-2779 Haskell . I. du Font de Nemours and Co*, Inc. Laboratory for Toxicology and Industrial Elkton Road, P. 0. Box 50, Newark, Delaware 19711 Medicine HASr':LL LABORATORY REPORT NO. 12-82 Material Tested* Octanoic acid, pe^tadecafluoro-, ammonium sale HaskeLl No. 12,037 Other Codes C-8 14,053 Nonanoic acid, 2,2,3,3.4,4,5,5,6,6,7,7, 8,a,9,9-hexadecafiuoro-, ammonium sale 14,075 Scudy Initiated/Completed ll/l//81-12/3/yi "CR&D Department Haskell Laboratory FUURTELN-UAY FEEDING STUDY IN MALE AND FEMALE MICE Summary; Cri:^1'''-! fenale and male mice were led feither (3) ammonium omega-hydrohexadecafluorononanoate, or (4)l1 SSg^SltKt 30 ppm, 300 ppm and 3000 ppm for 14 consecutive days. All female ffSff and male mice died after dosing with C-8 and ammonium omega-hydrohexadeca- t luorononanoat-e at 3UUO ppm. Deaths also occurred at 3UU ppm wit^ C-8 anfl .aimponium omega-hydrohexadecafiuorononanoate, and at 3000 ppm with find Body weight loss was indicated at 3UOO ppm and 300 ppm with all 4 test materials, and at 30 ppm of ammonium omega-hydrohexadecafluorononanoate. Liver weight/body weight ratios showed a sharp increase over controls in female and male mice, dosed at 30 ppm with C-8,^ammonium omega-hydrohexadeca- tiuorononanoate and ^^^"^TM^"""^"""^ Dose-response increases were 1 - Company Sanitized. Does not contain TSCA CBl aria-.-asiSS^iSS - -.W-::it:E';-.y^.---g--St^i^; UBHmi^lBlBice seeninC-8,j^^^Hland sahomovoendiumno odmoesgea--rheyldartoehdexinacdreecaasfleusoroiunolnivaneoratwee. xJgjh|C_/f_io_d_y' weight ratios froa30 ppn to 300 ppa, however, they did show a dose-repone increase at 3000 ppo. Female and aale mice fed C-8 and a--oniua oega-bydrohexadecaf luoronouanoate bad heavier liveca at lower concentrations than ice ^lofs^sf,Linakaemapatmhmeoandiumareoam,egwae-ahkyndersosheaxnaddedceaaftluho. rp|tnfoBnaBnoHatHe liMncMlud--ed--; --weimghUty clinical signs included: weight loss and deat-h. In female and male mice, ammonium omega-hydrohexadecafluorononanbate withpUUlUUU------U produced greater liver weight changes at lower concentrations than the other materials tested, followed closely by C-8. All mice died at 3000 ppa withC- 8 and aiijponium .Qinega-hydrohexadecafluorononanoate^^^pemousedied at 3000-- ppn witHUflBland 2 mice died at 3000 Ppm One deatfr occurred at 3UU ppm with C-8 and 5 deaffis occurred at 300 ppa itn ammonium omega-hydrohexadecafluorononanoate. at 30 ppm with any of the materials tested. No deaths occurred after dosing Procedure: Eighty female and 80 male Ckl^CDP-l mice (age 43 and 44 days, weighing 21 to 38 g) were grouped by body weights into 4 groups of 5/sex/ test material according to computerized randomization. For 14 consecutive days, the mice were housed individually and fed either ammonium pert luorooccanoate (Hffi 2,037 ) ^lBBBI^#l4,053), ammonium omega-hydro- hexadecafluorononanoate (Hfrl4,0?5), or^H|BBBBiMIIBBMHB9| (^^fO^) mixed with GPLC (ground Ririna Lab Chow^ Each material was fed At 0 (controls^ 30, 300 and 30UO ppm to female and male mice. The controls were fed GPLC only. Individual body weights were recorded 3 times/week, clinical signs daily and liver weights at the 14-day sacrifice. Kesulcs: o Female Mice Concentration PPm C-b H(fl2,U37 Average Average Body Weights (g) Liver Weights (g) 0 3U - 300 3UOU 26 27 18(1-0)** A.D.*** 1.31 3.35 2.98 ^14,053 Average Average Body Liver Weights (g) Weights (g) 26 26 25 23(1-D) 1.44 1.66 2.53 4,20 2 - Cpmpwv San^ao. 5^ " ^"rr^: -"^'! ss '.'^s Results: o Female Mice (Cont'd) Concentration Ppa Ammonium onega- hydrohexadecafluirononanoate (ltfl4.075) Average Average Body Weights (g) Liver Weights (g) l^ (ltfl4,068) ' Average Average Body Weights (g) Uver Weights (g) 0 24 1.37 24 30 25 4.18 24 3UU 20(3-D) 5.11 24 3000 A.D. 20 1.40 2.68 2.54 3.21 o Male Mice Concent rat i-n ppm C-b (HiS42,037) Average Body Weights (g) Average Liver Weights (g) Ol#14,053r Average Average Body Weights (g) Liver Weights (g) U 30 3UO 3uUO Concentration ppm 32 33 24 A.D. 1.76 35 4.06 33 4.16 37 27 1.84 2.15 3.63 5.16 Ammonium omega- hydrohexadecatluorononanoate (H?/14,075) Average Body Weights (g) Average Liver Weights (.g) r ^ ^ . ' l llllfBBBBRj (m?14,068) Average Average Body Weights (g) Liver Weights (g) U 30 300 30UU 33 30 22(2-D) A.D. 1.77 5.14 4.19 34 33 33 28(2-D) 1.96 3.54 3.70 4.51 o Average Liver Weight/Body Weight Ratios (g/100 g Body Weight) Concentration ppm Htfl2,037 Female Mice Hfrl4.053 ?4,075 HH4,068 0 30 300 3000 5.04 12.40 16.56 5.54 6.38 10.12 18.26 5.71 16.72 25.56 5.83 11.17 10.58 16.05 iiisfclsfeaai:!!^ 3 - Compaq San^d-Oo^""^"081 aa^  Concentr<ation PPB 0 30 300 3000 ltfl2,037 5.09 12.30 17.33 Male Mic:e Hl4,053 Hl4.075 5.25 6.52 9.81 19.11 5.36 17.13 19.05 ~ '^"n-j-^p^ IB '^N^ Hih4,666 5.76 10.73 11.21 16.11 ''^jt :^' '1 ?''':- Clinical Observations: Concencra tion ppm c-y U None 3U None ^"-\ None None 3UU 3'JUU Weight loss, unkempt head Weight area, weakness , death loss Weight loss, Weight loss, death, unkempt unkempt head head area area, death Ammononium c ^ -- onega-hydro- hexadecafluorononanoate None None Weight loss, weakness None Weight loss, unkempt head area, death Weight loss Weight loss, death, unkempt head area Weight loss, death .1 '' Hff12,037 Other Names: o Ammonium perfluorooctanoate Hfr-14,053 Purity; Contaminants: CAS Begistry: Other Names: Contaminants: - 4 - t-^ J^T 1^14,075 Other Names: o Ammonium omega-bydrohexadecafluorononanoate o C-9 ^ K^14,068 ^ Other Names: ftn-it-y: Composition: Contaminants: ** (//-D) = Number of deaths which occurred at this concentration. *** A.U. = All died prior to sacrifice date. Report by: 'Lynn S.i/Ford Technician Reviewed by: 0. Louis Dashiell Study Director Approved by; LSF:tac:WP:5.12 ^ Date Issued: March 8, 1982 Report No. 12-82 ja^^t -f. >6^^JL Y Gerald L. Kennedy,IJfr. Section Supervisor Acute Investigations Company Sanitized. Does net contain TSCA CB1