Document rpZ9pL1mZNZrLMyyMGNdNxL8q
AR226-2765
FOR DU POKT USE ONLY
Copies to:
E. I. 4u Pone de Nemoura and Company
Haskeli Laboratory for Toxicology and Induai-rlal Medicine Elkton ;Road, Newark, Delaware J9711
HASKELL LABORATORRYEPORT NO. 329-81
Material Occanoic acid,
T e s t e d H a s k e l l penCadecafluoro-,
o~ 12,037
ammonium sale*
Other Codes
C=5----------
Scudy Iniclaced/Coapleced ----5/26/fil - 6/11/81------
> Material Sub
IBB&b Departnen?
flankfll Laboratory
ORAL LP50 TSST IN HICE
Procedure; The teat materials as a upenclon la corn oil, wa adalnlafcred
by InCragasfcrIc Incubation in lingi* donee Co 6 groups of male and 6 group!
of fanale young adult CDO-1 alee, 10 per group* The turvlving alee vere
weighed and obaerved during a114-day recovery period and then sacrificed.
The LD50 value was calculated!from ehe Boreallty data using the method of D.
J. Finney.**
I
Reaulee:
Male*
Dose
(na/ka)
4,0001 2,0001 1,000
750 500
250
Average Bod y
Weight (a)
38 38 38 31 36
30
Suipe nlon
^ )
1 5
1 0
k 3 2 1
Average Doae (oL)
1.01 0.76 0.95 0.76 0.89 0.75
Mortality
Ratio 10/10 10/10 10/10 10/10 4/10
1/10
LD50*** 457 ng/kg
Company S.n.z.d. D^ no. co^in TSCA CB
Results;
Dose
(ma/kg)
Average Body Weight ()
Suspension
(X)
Average Dose (roL)
Mortality
Ratio
LD50***
Females:
4,000t
35
2,000t
34
1,000
33
15
0.94
10/10
457 ing/kg
10
0.67
10/10
4
0.82
10/10
750
25
3
0.62
10/10
500
33
250
24
2
0.82
4/10
1
0.60
1/10
Clinical Signs:
Males: Stained and/or wet perineal area, weakness and weight loss in all
surviving mice, eyee half closed at 1,000 and 250 mg/kg. Ruffled fur at 250 and 500 mg/kg. Acaxia at 1000, 500 and 250 ag/kg. Tremors at 500 and 750 mg/kg. All deaths occurred within 6 days after dosing.
Females: Stained and/or wet perineal ares, weakness and weight loss in all
surviving mice. Eyes half closed, ctalned face and tremors at 750 mg/kg. Acaxia at 500 mg/kg. Ruffled fur at 750 and 250 mg/kg. All deaths occurred within 7 days after dosing.
Summary; Ammonium perfluorooctanoate is moderately toxic when administered
orally to young adult CD-1 male and female mice; its LD50 for males and
feoiales is 457 mg/kg of body weight. Clinical signs included: stained and/or wet perinea' area, ataxia, tremors, weakness, ruffled fur, eyes half-closed and weight loss. All deaths occurred within 7 days after dosing.
Finney, D. J,, Probit Analysis, 3rd Ed., 1971, Cambridge University
Press.
2 -
CoH'psr.'/ SanWsed. Dcs^ no confab TSC^ CEs;
*** 95X Confidence Limits;
Males: Lower: Upper: Slope:
344 ng/kg 560 ng/kg
6. 6
Females:
Lower: Upper; Slope ;
344 ng/kg 560 ag/kg
6.6
4000 LD50
and 2000 daca due
mg/kg to
dose
levels
male
and
female
were
not
calculated
in
limitations of computer.
Report by:
u
L M ^ / John A. Hall Technician
Reviewed by;
Study Director
JAH.-jrg Date Issued;
June 17, 1981
''Report No. 329-81
"~uerald ^^Jy Approved
by:
Chief,
-c^i_..l ^.
L. Kenne4y
Acute Investigations
Section
- 3
^con^. "T^0SC^
Sa^10035
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