Document VGgOd87wK1LDp2jQ3Ob7bygj8

AR226-2753 Copies Co Material Tested Uccanoic ac id, pentadecafluoro- , ammonium saic* E. I. du Font de Nemours and Company llaykull Laboratory for Toxicology and industrial Medicine Elkton Road, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 659-79 Haskell No. 12,037 Other QwCodes ] Study Initiated/ Completed 8/21-9/12/79 (rabbits) 8/22-9/11/79 (rats) Material Sub | ^J^lBBU|plastic ----DepartEent^washingt o SKIM ABSORPTION LD50 - RABBIT AND RAT Procedure: (Rabbits) Four groups of male albino rabbita were clipped free of hair over the back and, trunk area an plastic collars. The proper dose of test material (made into a slurry with water) was applied to t and wrapped successively with layers of Sairan Wrap, Kling gauze bandages and Elastoplast elastic hours of exposure, the animals were unwrapped, washed with water, dried and returned to cages for 1 vation or until death. During the observation period, the animals were weighed and observed for cl pathology was done on all survivors and 1. animal that was found dead at 3,000 mg/kg. rim die mortality data using the method of D. J. ^inney.l The LD50 valu 1'njccdure: (Rats) Three groups of ChR-CU male rats 5 rats per group, were clipped free of hair over the back proper dose of test material1, as a 50/50 aqueous suspension, was applied to the back or each rat u aluminum foil and held in place with 3" Elastoplast. After 2A hours exposure, the rats were unwra a mild det-crgtinc, rinsed, dried and returned to cages for 13 days observation or until death. Duri period, i:ht- rats were weighed and observed for clinical signs. Gross pathology was done on all su dicj ac 7,500 ing/kg. The LD50 values were calculated from the mortality data using the method of D one rat closed at 7,500 ing/kg did not havu liver wytght recorded at pathology, company Sanitized. D V. -^y -^ . liLK C659-79 1 - - , KdauUs: ;,j. or Animals 2 5 5 5 (Rabbics) Dose (nig/kg) Average Dose (g) (Aqueous Slurry) Average Weight Initial/Final , Mortality1' Clinical Sig 7,500 5,000 3,000 1.50& 25.28 16.63 10.39 4.99 3370.5/ 3326/2872 3463.2/2827.2 3320/3002 ID ID - 20- 1D 2S - ID- 2S 2S - 5S 2 d. 3d. 3d. Ad. 14 d. 7 d. 14 d. 15 d. 14 d. Weight loss, labored breaching underneath and moderate skin i Nasal discharge, shallow breat rhea, wet underneath body, wea and skin irritation with necro Labored breathing, lethargy, s weight loss. Lahore* breaehiag, skte irrita area and weight loss. LD50 = 4,278 ing/kg 95% Confidence Limits; Lower: 2,369 mg/kg Slope: 6.2504 Upper: Kcdulcs: No. or 'Animals 5 (Rats) Doae (mg/kg) 7,SOL 5 5,000 5 1,000 Average Uoae (g) (Aqueous Slurry) Average Weight ^ Initial/Final Mortality' Clinical Sig 1.9 Gross Pathology: 254 / 298 3D - ID IS - 1 d. 2 d. 14 d. Lethargy, chroinodacryorrhea. s stained perineal area and init 2 rats: 1 FD at 1 day - liver weight not taken. No other c signs noted. 1 Survivor (14 days) - increase in liv 1.4 Cross Pathology: 276 / 316 5S - 14 d. Lethargy, wet petineal area an 5/5 Rats; Increase in liver weight. 0.87 Gross Pathology; 290 / 345 5S - 14 d. Initial weight loss only. 5/5 Rats: Increase in liver weight. LD50 = 6,959 ing/kg 95% Confidence Limits not defined - Slope: 25.9 , Company San.liz8d.Do - 3 Siiiiunary: (Rabbit a) Orlanoil: acid, penladucafluoro-, ammonium salt ia moderately luxic by skin absorption in rab 4,278 nig/kg of body '..'=-ighf:. Gross patliologi.t-al examination showed no compound-related changes; however, there was skin i levels. Clinical signs included weight loss and labored breathing at all levels and wet underneath diarrhea and lethargy appearing at mos' levels. Summary; (Rats) Octanoic acid, pentadecafluoro-, ammonium salt is slightly toxic in male rats by skin absorp 6,959 mg/kg of body weight. All racs dosed at 7,500, 5,000 and 3,OUO mg/kg had initial weight loss. Rats dosed at 7,500 yrire lethargic on the day of treatment aa\Q the majority had wee and/or stained perlneal areas. Cli seen in rats treated at 7,500 mg/kg. Cross pathology showed an increasie in liver weights in all surviving rats examined at 14 day t)Fli/JEH:scg ltdporc No. 659-79 Date Issued: October 26, 1979. w '^ Keis.sjucil: February 2C,7!f Report by: /^ <-- ^ ^'-^ Doris F Toxic S.b^nt, i^xv John E Techn Approved by: Gerald Chief, Acute Inv Oosn Company Sanded.