Document KQ23yqjL5qpeLpVV7NY3dGv6

AR226-3074 Study Tit-le Inhalation Toxicity Screen withj Laboratory Project ID HL-1997-00599 Author David P. Kelly Study. Completed July 11, 1997 Performing Laboratory Haskell E. I. du Pont de Nemours and Company and, Industrial Laboratory for Toxicology Box 50 Elkton Road, P. 0. Newark. Delaware 19714 Medicine Company Sanitized. Does not contain TSCA Cffl Du Pont HL-1997-00599 Inhalation Tox-'city Screen wit An inhalation roxicity screen was conducted with six male rats exposed for four hours to H-22484 according to Standard Operating Procedure No. AI005-T. The test substance was aerosolized riith a Spraying Systems Nebulizer. The aerosol atmosphere was analyzed by gravimetric analysis of filter samples. After filter samples were collected, the filters were immediately weighed to determine the wet aerosol concentration. After overnight drying in a desiccator, the filter samples were weighed to determine the dry aerosol weight. The mean aerosol concentrations during the four-hour exposure were 410 mg/m (wet) and 400 mg/m (dry). The aerosol mass median aerodynamic diameter was 4 pm. During the exposure rats showed a diminished alerting response. No deaths occurred during the exposure, however all rats died within four days of exposure. Upon unloading rats from the chamber, clinical signs of toxicity observed in the rats exposed to H-22484 were irregular respiration and lethargy. Three of six rats died approximately two hours after exposure, one rat died on the day after exposure, one rat died two days after exposure and the remaining rat died three days after exposure. After the exposure rats continued to lose weight until they died. Clinical signs observed during the recovery period included lethargy, hunched posture and irregular respiration. Experimental details of the study are in laboratory notebook------------ Characterization of Chamber Atmosphere ----------Chamber.Aerosol-- Mean Cone. SD Range (rag/m ) 400 (dry) 29 360 - 440 Particle size Analysis MMAD GSD %<lum %<3um %<10um 4 2.2 4 35 88 Mortality Deaths/Exposed 6/6 -T^.AJ^A^ Work by: -7,1/^7 MM. A& . DP<ui1ll1iajamm, BB.SS . Toxicology Technician ^^^1^^^:^7/A rwwy Study Director: I 9 7 Kelly, B.S. Inhalation an&^ellular Toxicology Issue date: July 11, 1997 MMAD = Mass Median Aerodynamic Diameter(urn) GSD = Geometric Standard Deviation Company Sanitized. Does not conlain TSCA CB|