AR226-2852 PERSOWAL AND CONFIDENTIAL Copies l:o:l E.

FOR.PO' FONT USE ONLY AR226-2888 Copies to E.

Study Sponsor: Issue Date: Document Reference No.: ELFATOCHEMS.A.

AR226-3061 Study mio TOP Biocfflnpatibility Testing of S-22072.

TRADE SECRET AR226-3084 Study Title H-23004: ORAL APPROXIMATE LETHAL DOSE (ALD) IN RATS LABORATORY PROJECT ID: HL-1997-01291 AUTHOR: Tracy A.

AR226-3121 I *j ~Vl^l'K9^ Huntingdon . - .-._, f^_ ^^........^^.-^.-..s^.^^^^^^^^'^^^'^^^?

AR226-3204 DuPont-2925 Supplement No. 1 TRADE SECRET Study Title H-24000: Biopersistence Screening 10-Dose Oral Gavage Study in Rats Supplement No. 1 Laboratory Project ID: DuPont-2925 AUTHOR: Carol Finlay, B.A.

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AR226-3339 --------------------^^ Roy S Thompson (AstraZeaeca, Brixham, UK) Haria Colombo Watze de Wolf (Seh'ay Sotexis, BaHate, Stely) (OuPoBt, Mechelt'n, Belgiaaa) David G Fanrar Robert A Hoke Jacques L'Haridon (laeos C&ior; Ruacorn, UK) (DaPont, Newarfe, BE, USA) (CST, Evreux, Fraace) for.

c SaKaitfheVriisenePEr.esRideeent, PhD. : off FN Coen 3SaMfetEymiOpreornamteinotnsal Heath and ne Haze. 3SHE O5SEP-2 0:38 ne S79C000PBBaauxlsh3MA0Nv1e$ne5,13B3u.il3d1ing 4225.26 )ST a 258765 August 26,2005 Teh-otos-037 RDoocoumme6n4t28Processing Center (Mail Code 7407M) NO TSCA CBI OAtftfeincteioofnP:olTlSutCiAon8P(r)evCeonotridoinnaatnodrToxics US.

- 9 Ape 22,3003 Ch0riMs 1mShaaman Ar22-1692 StfH EoPssHenBduigdainnegrs r0 mpaonm DmE Seioen, Dese Ms Whitman, As today ionmota EaaieDyy,o wseiehmssassopaeclieadlmaapptriooprninmeefogrmeh0e sreius e{ommorniumea Peerfluorioeohcstsapnsoaotmety(Cse8en)cropanrndombeelneetmtapl1erbpoestursa(ttehdeorntthee uensuespedcti$n1g5p0ub1l)ic by 1 worked foratshoe DuncPoonvtoeCmoemnpgaemnryvoufocsrhotmsaennoy yesnarestaonaodayhoaruvesandoiirmeyocmtoklunsaolnwylSebdiegceaeouesfeot|hseeirhre for my safety as a result ofwhl this company might doo meifmy identity were known `While I worked olan 0 management snd wh regrly etend by thir for them, like some other Dupont workers, I regularly tried to do the right corporante "isseecuroibtayco"kfigtwoohoenonpsutufbhoeirydcdopoeniugangbtaslno.ys[ohdunniydhonmfoyptwohiwinhneaognruwttrdotsonopigDe.nunpdTooihnnnetlayrnheydelemmyoynaencyda.4nedfaTrhtkhedaeitfatehreeynt O altruistihcn eDnfuplooonotkohissapvaswrhotraoretfpetshtahcoyeendpCpacsotor.rpiiRorannabtie1Bdg0tidnc.oym1pyetaevnnOrdcyeh,iownnHredoesintfygf.eorTenhdcieyassnDdauponovnertatndNo Ee Their actions are et de. corr and the direct result of informed aonhdedrealiibsercarteeudemcuisnitonhsebyegDaulPpornotces 5 `pertains to the use of and the environmental release and/or disposal of C8.

Summary PFOS Rat Two-GenerationReproductionStudy StudyNumbers:3M T-6295.9,Argus418-008(in-lifFeA)C,T-TOX-012 (analytical).

CENTRE FOR XENOBIOTIC RESEARCH UniversityofDundee, BiomedicalResearch Centre,Level5,NinewellsHospitaland Medical School,Dundee, DD1 9SY Study Report Determinationof cyanideinsensitivPeaimitoyi-CoA oxidationinsamples from 3M Environmental Laboratory - Covance Studies6329-183 and 6329-212 Study Number XR0108 Author: Approved: t.M.Elcom,be C.R.EL .e- XR0108 PaLyeI of 3 Date: I Introduction The purposeofthisstudywas toinvestigattehelevelsof PCOA oxidationinthefrozen liversamplesprovidedby 3M EnvironmentalLaboratoryfrom Covance studie6s329-183 and 6329-212. 2 TestSubstance 6329-183:PFOS, 20ppm, administeredby Covance untilweek 4. 6329-212:ETFOSE-OK 100 and 300ppm, administerebdy Covance untilweek 4. 3 TestingFacility CentreforXenobioticResearch,UniversityofDundee, BiomedicalResearchCentre, Level5,NinewelisHospitaland MedicalSchool,Dundee,DDI 9SY, UK. 4 Homogenste Preparation 4.1 Reagents SET buffer0:.25M Sucrose,5mM EDTA and 20mM Tris-HCI,pH 7.4 4.2 Instruments Potter-Elvehjehmomogeniser 4.3 Method Frozen liversampleswere thawed on ice,blottedand weighed.A 25% homogenate was preparedinicecoldSET usinga Potter-Elvehjehmomogeniser(6passes). 5 Determinationof Cyanide-in.sensitPiavlemitoyi-CoOxidation The 25% homogenate liversampleswere dilutedI partin 10 partswithicecoldSET.

PerfluorosulfonamiMdeetabolisminRatvs..Hum-an--Heya R C H CH S 0 -2N -%.

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanesulfonyflluoridem;ay also be referredto as POSF, or FX-8. (1-Octanesulfonylfluoride, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluCoArSo-#, 30735-7).