Document X7OXgbQmvg5a5J3o3D3RvGD0d

AR226-2930 FOR DU FONT USE ONLY HLR 415-85 HASKELL LABORATORY REPORT NO. 415-85 Title of Report ________ STATIC ACUTE 96-HOU?, LC501 OF TO THE FATHEAD MINNUW, Pimephales promelas "askell E. I. du Font de Nemours and Company, Inc. Laboratory for Toxicology and Industrial Medicine P. 0. Box 50, Elkton Road Newark, Delaware 19714 Date Issued: September 17, 1985 Company Sanitized. Does not confam TSCA CBf HLR 415-85 Title of Report STATIC ACUTE 96-HOUR LC50 OFl TO THE FATHEAD MINNOW, Pimephalea promelE Summary I^^^BU exhibited low acute toxicity to a warm freshwater fish, the fathead minnow, under static unaerated test conditions during a 96-hour exposure period. The 96-hour LC50 was 2341 mg/L, with a 95% confidence interval of 1766 mg/L to 2743 mg/L. Report by: Study Director: _^ \ A -^/ / t^y, James W. Wetzel Technician ^. T^dJtp^ 6-. 'S'h^ug."T7. Ralph G. Stahl, 3r. Research Aquatic Toxicologist Z i 2 r Mo/Day/Yr ^/i?-|gs Mo/Day/Yr Approved by: '-'-^-//ii 'i'- r-r^s^''c/_____ Carol.0. Litch field Section Supervisor, Environmental Biology 3 M o ^'^ /Day/Yr There are 7 pages in this report. - iCpmpariy Saiimzed. Doua not conlai.i TSCA CBi Haskell Laboratory Report 415-85 HLR 415-85 Material Tested Synonyms Haskell No. 15,866 Other Codes CAS Registry Number CompositionF Sponsor STymer Products Department Material Submitted by 'olymer Products Department Washington Laboratory Parkersburg, W. Va. Test Facility E. I. du Pont de Nemours & Company Haskell Laboratory for Toxicology and P. 0. Box 50, Elkton Road Newark, DE 1971 A Industrial Medicine Study Initiated/Completed Initiated on 6/17/85 and completed on 7/12/85. There are 7 pages in this report. Distribution I^BffipanSyanlfizeg. Voesnofconiam TSCA CB8 3 - HLR 415-85 Introduction/Purpose af^ffiff To determine the static acute toxicity (H-15,866) to a warm freshwater fish, the fathead minnow (PimephaleB promelas). Materials/Methods (Environmental Biology Section Aquatic SOP-T02) The test material ,^H|H|^f was received as an aqueous slurry containing concentrations and the stock solution are based on the weight (density of slurry: 1.2 g/mL) of the aqueous slurry and were not corrected for the amount of active ingredient. The test material, _ f ^^ ___^ ^^^H ^^^^^^^^_ _fK, was prepared as a 30 mg/mL stock solution in distilled deionizedwater^The pH of the stock solution was adjusted to 7.0 with 1N NaOH. Appropriate volumes of the stock were added to 3-liter all glass aquaria and diluted with laboratory well water to yield the desired nominal exposure concentrations in 4-liter final volumes. Two identical vessels containing only laboratory well water were designated as the H,0 controls. Stability of the test and control materials in well water was not determined because it is not a critical factor in static acute aquatic tests. Five juvenile, unsexed fathead minnows from Haskell Laboratory-bred stock with a 2.08 cm mean standard length (range 1.6 to 3.1 cm) and 0.136 g mean wet weight (range 0.023 to 0.440 g) were randomly placed in the test aquaria. The fish were maintained at Haskell Laboratory for 126 days before being used for this test. Haskell Laboratory-bred fathead minnows are identified by labeling the culture and test vessels. Fish were not fed for 48 hours prior to nor during the exposure. The test solutions were not aerated, and temperature was maintained between 21.1 and 22.0C. Photopsriod was maintained at 16 hours light; 8 hours dark. Mortality counts and observations were made every 24 hours during the 96-hour exposure period. Dissolved oxygen and pH were measured in the H-0 controls and in the low, medium, and high test concentrations at the beginning of the test and at 48-hour intervals during the 96-hour exposure period. Total alkalinity, hardness (EDTA), and conductivity were measured at the beginning of the test in the HyO control. The 96-hour LC30 and confidence limits were calculated by probic analysis (Finney, 1971). All records from this teat and the final report will be maintained at Haskell Laboratory for Toxicology and Industrial Medicine or archived at the Du Font Hall of Records. ywpu-l, ^^2ss. Uv^ Mtconi&in TSCA CB HLR 415-85 Results fUMHB Test results for fathead minnows exposed to^lUHB(H-l5,866) are presented in Table I. exhibited loS^acutetoxicifcy to fathead minnows under static unaerated test conditions during a 96-hour exposure period. The 96-hour LC50 was 2541 mg/L, with a 95S confidence interval of 1766 mg/L to 2745 mg/L. The chemical and physical parameters measured during the test of H-15,866 are reported in Table II. All were within acceptable ranges for this study. Discussion/Conclusion |BUUf(H-15,B^>) has been tested for acute koxiciky with the fathead minnow, a warm freshwater fish. Under the conditions of this test, BBwas found to exhibit low acute toxicity. Footnotes LC50 = That concentration which is lethal to 50% of a test population during the specified time period. References Finney, D. 3., (1971). Press, 555 pp. Probit Analysis, 5rd. Ed., Cambridge University 5 ' Company Sanitized. Does not contain Tpc HLR 415-85 TABLE I RESULTS OF A 96-HOUR STATIC ACUTE TOXICITY TEST WITH FATHEAD MINNOWS EXPOSED TO H-15,866. Nominal Test Concentrations (mo/D 6400 3200 1600 800 400 200 H0 Control 24 Hr. A* B* 100 100 100 100 0 0 0 0 0 0 0 0 0 0 Obiserved Mortal ity (S;) 48 Hr. 72 Hr. A* B* A* 3* 96 Hr. A B* 100 100 100 100 100 100 100 100 100 100 100 100 0 20 0 20 0 20 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 *Replicate exposure chambers containing five fathead minnows each. - 6 - .,..-..^^^?"'Kr ^ ^ p -- -a- n.^.'ytSfb^a.aTHits-H,e^d1-.,O- -yr'i-'-"^ pf, -1 no1 -' -..l.JS^ t^' HLR 415-85 TABLE II RESULTS OF PHYSICAL AW CHEMICAL PARAMETERS i mri*fa- fc-rw MEASURED DURING A 96-HOUR STATIC ACUTE TOXicinyrsT^^ WITH FATHEAD MINNOWS EXPOSED TO H-15,866 Physical/ Chemical Parameter s Dissolved Oxygen (mq/L) 0 Hr. 48 Hr. 96 Hr. H 0 Hr. 48 Hr. 96 Hr. Total Alkalinity (mq/L as CaCO-,) 0 Hr. EDTA Hardness (mg/L as CaCO,) 0 Hr. Conductivity (umhos/cm) 0 Hr. 6400 (Hiqh) Nominal Test Concentrations 800 (Medium) 20 (Low) Controls H,0 8.4 8.4 6.6* 6.7 6.2 6.9 7.3 7.0* 7.3 7.4 8.3 8.3 6.8 7.5 5.9 7.5 7:3 7.4 7.3 7.3 7.3 7.4 84 76 166 * Measured at 24 hours due to total mortality. - 7 - Solflpany Sanitized. Does not contain TSCA C9f