Document o9w7LVMZb6pveLLeJk4jqeDk7
TRADE SECRET
AR226-3212
Study Title H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus
mykiss
Laboratory Project ID: DuPont-8861
AUTHOR: Laurie D. Bouchelle
STUDY COMPLETED ON: December 21,2001
PERFORMING LABORATORY:
E.I. du Font de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050
Company Sanded. Do^nc. contain TSCA CB1
H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus mykiss
DuPont-8861
CERTIFICATION
We, the undersigned, declare that this report provides an accurate evaluation of data obtained
from this study.
WortCompleted by;
M(^A^.^ffS\)J^j^ La__u_r_i!e^ DT-k. Bo-_u-c--he1l1lc.
I T Associate Scientist
Issued by Study Director:
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Alan Samel, M.S. Research Ecotoxicologist
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Date
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H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus mykiss
STUDY INFORMATION
Substance Tested" Synonyms/Codes:!
Submitter's Notebook Numberfs):' Haskell Number: 25134
CAS Registry Number!
DuPont-8861
Physical Characteristics: Dark brown solid
Sponsor:
E.I. du Font de Nemours and Company Wilmington, Delaware 19898
U.S.A.
In-Life Initiated/Completed: December 10, 2001 /December 14,2001
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CompanySanitized. Doss not contain TSCA CBI
H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus mykiss
DuPont-8861
A
SUMMARY
The acute toxicity of H-25134 to the rainbow trout, Oncorhynchus mykiss was determined in an
unaerated, 96-hour, static test.
The study was conducted with 4 concentrations of H-25134 and a dilution water control at a mean temperature of 11.3C (range of 11.2-11.3C). One test chamber was used per test
concentration with 10 test organisms in each chamber. Based on visual observations, the water control was clear and colorless, and the 1.0, 10,100 and 1000 mg/L test concentrations were
clear and colorless with undissolved test material on the water surface and on the bottom of the test chambers at test start. All water quality parameters were within acceptable limits during the
exposure.
Exposure of H-25134 to the water control and nominal H-25134 concentrations of 1, 10, 100,
and 1000 mg/L resulted in 0, 0, 0,0, and 0% mortality, respectively, at the end of 96 hours. No
mortality or sublethal effects were seen in the water control test organisms. The highest nominal
concentration causing no mortality at test end was 1000 mg/L.
The results are summarized as follows:
Nomina concentrations of H-25134, mg/L 96-hour LC5o for H-25134, based on
nominal concentrations, mg/L
dilution water control, 1, 10, 100, and 1000
>1000
H-25134 exhibited low concern for aquatic hazard" in an unaerated, 96-hour, static, acute test using the rainbow trout, Oncorhynchus mykiss.
Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASTM STP 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia.
-4- Company Sanitized. Does not contain TSCA C
H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus mykiss
DuPont-8861
TABLE 1
DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES
Nominal H-25134 Concentration (mg/L)
Test Initiation (0 hours)
Dissolved
Oxygen
(mg/L)
PH
Test Completion (96 hours)
Dissolved
Oxygen
(mg/L)
PH
Water Control
10.6
7.4
8.7
7.3
1.0
10.8
7.5
9.1
7.3
10
10.8
7.5
8.7
7.3
100
11.0
7.5
8.8
7.3
1000
11.1
7.6
9.0
7.3
TABLE 2
MORTALITY OF Oncorhynchus mykiss, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-25134
Nominal H-25134 Concentration (mg/L)
Water Control
1.0 10 100 1000
Number of Dead Test Organisms /
Number of Test Organisms at Study Start
24 Hours
48 Hours
72 Hours
96 Hours
0/10 0/10 0/10 0/10 0/10
0/10 0/10 0/10 0/10 0/10
0/10 0/10 0/10 0/10 0/10
0/10 0/10 0/10 0/10 0/10
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Company Sanitized. Does not contain TSCA CB