ATTACHMENT TO LETTER TO C.AUER DATED AUGUST 31,2000 SUPPLEMENTAL SUBMISSION N-ETFOSA N-ethylperfluorooctanesulfonamide AcuteToxicit 1.

ARRR6 _ S412 --_ 16 '~ 000202 AR 226 = 34/4 ~D ; -- prkvi-- pasy EEBRUMRY, 1960 westr Jr-- SITENAME Exposure _A __n _Bla oodlysis Conc.

siotf ARR_ R Ju6 y3 i a7 000410 : ~oD ARRRG - 3442 TEFLONe FINE POWDER/DISPERSION OPERATORS C-8 BLOOD SAMPLE RESULTS | | | i " AY BD C-8 in Blood First Sample Second Sample 26.0 20.0 24.0 25.0 11.0 8.2 9.4 7.9 8.2 6.5 7.0 7.7 6.9 5.9 5.6 4.2 iss rx 4.3 5.6 3.9 3.6 3.6 2.7 3.5 2.1 2.9 2.8 2.8 2.8 2.6 2.4 2.5 1.8 2.5 2.5 2.1 1.6 1.6 1.5 1.5 1.2 13 1.1 (ppm) Change 6.0 +1.0 -2.8 to-L5 -17 +0.7 1.0 ~1.4 0.5 +1.3 ~0.3 ~0.9 ~1.4 -0.1 0.0 0.2 -0.7 0.0 0.5 -0.1 -0.3 -0.2 JWR:1dr/10/19/83 Days Between Samples 363 364 391 364 363 364 337 334 365 401 364 362 363 357 365 391 336 349 288 384 366 362 % Per Year Change -23 + ~24 -16 -21 +10 16 -27 .

v2 , REEEEEENER ny f - QUPIND OPEL sree 75?

August 21: 2000 Document Processing Center (`7407) Office of Pollution, Prevention and Toxics U.S.

. ui June to. 2000 Document Control Office Docket AR-226 WE1a2Ps0hA1inECgaotsnotsntBi,utiuDltdiCionng2.0AR4ve6o,0oNm W6428 Phone: 202-564-8930 Attention: OPPT Docket No.

AR226-012] FE-RY TOXICITY TO AQUATIC PLANTS (E.G., ALGAE) TEST SUBSTANCE -- Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95 or aspartof the mixed product FM-3820 (see Remarks). (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8.8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test sample is FM-3820, a mixture of the test substance in diethylene glycol butyl ether and water (approximately 24-28% test substance in diethylene glycol butyl ether and water).

Attachments to Letter to C.

PD HAe ZLo ETO.N FEcTEoWe~mAaSmOcWNnISnCOeNSIN 357 + Chemica & Bioadiet Sconces PH6O0 N24E47 + TUX TESS NAZRAL Division MDS UD FINNAALL RERPEOPFORT PJTAoNNXEIISNCEOOLToGABLYERMSISONENIRNVGICE&SMANUFACTURING COMPANY TERS sion savpLes 1-352 PURCHASE ORDER NUMBER: T357842, REL. 4513 i{ qersmws : on PE . ; Sk SAMPLE NUMBER: 50503501 SAMPLE ENTERED: 05/15/85 REFORT PRINTED: 06.21/85 ENCLOSED: PSBRaAIiUMADRREaYPtOaRETYAEPPEINRDRIIXTATION - METHOD, SUMMARY sioveo: SiBEorm e1, eGldaza.

T 3253.5 Ore Teratology Study of mi-3422 in nats Experinent Ko.: ---- Soatuatee Dosing Testes: study bisector: ossoroo0 SSBattteBtayum dv,alWuiano tmieosnorsLaboratory August 19 to september 4, 1980 2 0. contnee <A SeCnioSr Eesraeiscoh nTechnologis)t-282%-% AStianyaDRireecpeoertto rereieiagy RLe seEaCaze0 nm4Vevp8 eorminrpaB, ruWyde,Pdat5choloh1g-is2tt2-9,)eE HManaagesre,esPsathTOoleslet-etoxicolYog1y 3/541 Satery Prateneion beset 004233 1.

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REPORT EVALUATION OF THE MUTAGENIC ACTIVITY OF T5869 IH AN IN VITRO MANHALTAN CELL GENE MUTATION TEST WITH LSI76Y HOUSE LYNPHOMA CELLS (ITH INDEPENDENT REPEAT) NNOoTTOOXX SPurbosjteacntce11358915946 - page 1 of 22 - 005631 RECEIVED NOV -9 1004 TOXICMOeLOGY T5069 STATEMENT OF GLP COMPLIANCE NOOK Project 115954 \OTOK 6...

p= BE $Z--0Q3309//--1/3 78 A am DuPont Haskell Laboratory MR AR 216 _ 102g i-- E ewan ot ie ne 53ca30 GDierreacltdorL,.AKpeplnineeddyToxicology DP.uOP.onBtoxHa5s0kell Laboratory Elkton Road NPheownaer:k,(3D0e2l)aw3a6r6e-521599714-0050 FYI-90-801378 n" = Z=oaapE VIA FEDERAL EXPRESS i RDoocoummeGn9t9CEoansttroTlowOeffricAeT(T7N4:07)FYI - Office of Pollution Prevention and Toxics 4U0.1S.MEnSvtirreoctn,meSnWta.l Protection Agency Washington, D.C. 20460-0001 8501000001 Dear Sie/Madam: - evLoonons March 8, 2001 Pro ru O@TEIRS Ne 077 "This leter is in reply to Richard HL Hefters eter dated February 14, 2001 to me concerning the above-referenced FYI submission.

Ar226_ 20 Estrous Cycling and Natural Delivery/Litter Observation Data F1Estrous Cycling Data, From Data Analysis Presented at EPA on August 30, 2002 SsA CbreceseD s T24'30 250\09 a55f00 T2i5029 m500 'APFO Dose Group (mg/kg/day) Creletonss Lncdence ofCycle Lengh Per Group PID ":3 r -- s7>7 ---- 5} -- 575 --H75---- w53 5:3 'si:u %: 3?

Oct 02 02 03:29p : Ralph Cooper 519 se1-s138 pz AR 2' 26 _ 1198 +"<3@8:x" NZ tron NUaNtIioTnEaDl HSeTaAltThEaSndENEnVvIiRroOnNmMenEtNalTEAfLfePctRsORTeEsCeaTrIcOhNLaAbGorEaNtoCrYy Reproductive Toxicology Division Research Triangle Park, NC 27711 MEMORANDUM October 2, 2002 SUBJECT: Control vs.