Document 71nOMEyjaENvRYXN6zLvLvx1e

AR226-012] FE-RY TOXICITY TO AQUATIC PLANTS (E.G., ALGAE) TEST SUBSTANCE -- Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95 or aspartof the mixed product FM-3820 (see Remarks). (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8.8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test sample is FM-3820, a mixture of the test substance in diethylene glycol butyl ether and water (approximately 24-28% test substance in diethylene glycol butyl ether and water). Calculations were made to adjust test values using the upper limit concentration of the test substance (28%) in the test sample and no adjustment was made for the presence of the diethylene glycol butyl ether or water when noted below. These calculations assumed that all toxicity was due to the presence of the Perfluorooctanesulfonate substance. METHOD Method: OECD 201 Test: Static acute GLP: No Year completed: 1991 Species: Selenastrum capricomutum Source: Originally from the Culture Collectionof Algae at the University of Texas at Austin, maintained in culture medium at EnviroSystems Division, Resource Analysts, Inc., Hampton, NH. Analytical monitoring: Temperature and pH Element basis: Growth rate Exposure period: 96-hours Start date: 4/29/91 End date: 5/3/91 Test organisms laboratory culture: Algae cultures had been actively growing in sterile enriched media identical to media used for the test and `maintained at test conditions. Test Conditions: Test temperature range: 23.0 -23.1C 01685 Dilution water: Synthetic media prepared with sterile deionized water according to U.S. EPA standard, 1978. SDitlouctkioannwdatteesrtssooulructei:onNoprtenpoatreadt.ion: A primary stock solution was prepared in algal medium at a concentration of 1000 mg/L. The primary stock solution was proportionally diluted with algal medium to prepare the five additional test concentrations directly in the test vessels. Exposure vessels: 250 mL glass Erlenmeyer flasks capped with inverted glass beakers and containing 100 mLoftest solution. Agitation: Shaken continuously Number of replicates: three Initial algal cell loading: 1.0 X 10cells/mL Number of concentrations: Five plus a negative control Water chemistry: pH range (0 - 96 hours) 8010.3 (control exposure) 7.2-8.1 (1000 mg/L exposure) Test temperature range (0 -- 96 hours) 23.0-23.1C Light levels: (096 hours) 40 uEin/sec/m' from continuous cool-white fluorescent lighting RESULTS Nominal concentrations: Bk control, 62.5, 125, 250, 500, and 1000 mglL. Test Sample Element value: 24-hour ECso (growth rate) = <62.5 mg/L (CI not calculable) 48-hour ECso (growth rate) = 707 (500-1000) mg/L 72-hour ECso (growth rate) = 284 (249-325) mg/L 9966--hhoouurr NECOsEC(g(rgorwotwhthrartaet)e=) =251525(2m2g2/-L293) mg/L Perfluorooctanesulfonate concentration adjusted Element value: 24-hour ECso (growth rate) = <17.5 mg/L 48-hour ECs (growth rate) = 198 mg/L 9762--hhoouurr EECCssq ((ggrroowwtthh rraattee)) == 7810 mmgg//LL. 96-hour NOEC (growth rate) = 35 mg/L. All element values based on nominal concentrations. 001686 mStoavtiinstgicaavlemraegtehomdest:hoEd.CsThvaeluNeOsEwCerweacsalccaullcautleadteudsiunsgintgheDubninneotmti'asl toerst. Tnhuembaevreroafgceesllpsecpiefricmigraotw9t6h hroauterswamsincuaslctuhleatneadtuarsaltlhoegnoaftutrhaelnluogmobfetrhoef cells per ml at 0 hours divided by the exposure period. The percent change from control is calculated by subtracting the sample average tshpaetcivfailcugerobwytthheraatveefrraogmetshpeecciofnitcroglroawvtehrargaetespinectihfeiccognrtorwotlharnadte, dividing multiplying that value by 100. The percent change from control was used to compute ECs) and NOEC values. Control response: Not noted. Mean Growth data: [omy[oow w | -]| ConcNeonrtrmaatlion,| Numberof| Average Specific| Percont Ifibiton via mal [Cel per mL| Growth Ral (u) | Growth Rate Control [os[wooo| oom | = | | os [ooo| os| ow | [mo [ooo| oe| 2 | dOebtseerrmvianteidonbsy:mTehaensnuomfbdeirrecotf maligcarlosceclolpsi/cmlcoinunetaschwittehstavheesmsaeclywtaomseter. Reversibility of Growth Inhibition: Not noted. CONCLUSIONS `The test substance 96-hour ECs and 95% confidence interval for (S2e2l2e-n2a9s3t)rummg/cLa.prTihceom9n6u-thuomuwraNsOdEeCtewrmaisneddetbeyrmgirnoewdthbyraDteuntnoebtte'2s55 procedure to be 125 mg/L. If you attribute alltoxicityto the Perfluorooctanesulfonate component of the test substance (FM-3820) using the 28% upper concentration limit, the 96-hour ECs (growth rate) = 71 mg/L and the 96-hour NOEC (growth rate) = 35 mg/L 001687 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking = 3. The study lacks analytical measurement of test substance concentrations in the test solutions, sdeatmeprlmeinpautriitoyn,oafntdheinmsuofdfiecioefnttoexxipciltayn,aitnisounffoifcioebnst ecrhvareadctaenroimzaatliioens oifn toxicity readings. Additionally, the write-up did not indicate the test vessels were sterile, and data is foar mixture and toxicity cannot be positively attributed to Perfluorooctancesulfonate as the diethylene glycol butyl ether could also contribute to the toxicity. REFERENCES This study was conducted at EnviroSystems Division, Resource Analysts, Incorporated at the request of the 3M Company. OTHER Last changed: 5/3/00 001688