AR226-3089 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE H-23005: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals.

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BOD/COD resultstorFC-99 (DEA saltotPFOS) BIODEGRADATION TEST SUBSTANCE Identity:PerfluorooctanesulfonatDeE,A salt;may alsobe referredto as PFOS DEA salt,FC-99, or 3M Sample No. 2. (1-Octanesulfonic acid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafcloumoprdo.-, with2,2'-iminobis[ethan(o1l:]1)C.AS # 70225-14-8) Remarks: Testsample isa mixtureofthe testsubstanceinwater (approximately25% testsubstance and 75% water).Allvaluesreported relatetothismixture.No calculationwsere made to adjustforthe actual concentrationofthe testsubstance inthetestsample.

COVA-N'C-E-.@@ THE DMU FINAL REPORT CHROMOSOMAL ABERRATIONS IN HUMAN V%RHOLE BLOOD LYMPHOCYTES WITH PFOS AUTHOR Hemalatha Murli,PhD PERFORMING LABORATORY Covance LaboratoriesInc.

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C 0 V AV -C@-E.@ THE DEVELOPMENT SERVICES CO#APANY Sponsors: APME Ad-Hoc APFO ToxicologyWorking Group PROTOCOL Study Mde: 26-Week CapsuleToxicityStudywithAmmonium Perfluorooctanoa(tAePFO) inCynomolgus Monkeys Date: September 23, 1998 PerforniingLaboratory: Covance LaboratoriesInc. 3301 Kinsman Boulevard Madison,Wisconsin 53704-2595 Laboratory Study Identification: ProposalNo. w6806a Covance 6329-231 Covance 6329-231 Page 2 Study 26-Week Capsule ToxicityStudy with Ammonium Cynomolgus Monkeys Perfluorooctanoate(APFO) in Purpose To assessthe effectof the testmaterialon criticaelnzyme levels,hormones, and other selectedbiochemical parameterswhen administereddailyby capsuleto cynomolgus monkeys for atleast26 weeks Sponsors APME Ad-Hoc APFO Toxicology Working Group Study Monitor Paul Lieder,PhD, DABT 3M Toxicology Services Building 220-2E-02,3M Center St.Paul, Minnesota, 55144-1000 Telephone No.: 612.737.2678 FacsimileNo.: 612.733.1773 AlternateStudy Monitor John Butenhoff,PhD, DABT 3M Telephone No.: 612.733.1962 FacsimileNo.: 612.733.1733 Sponsor's Representative David Farrar,PhD ICI Chemicals & Polymers Limited Occupational Health The Heath, Runcom, PO Box 13 Cheshire,WA7 4QF Telephone No.: 01928.513953 FacsimileNo.: 01928.515146 Study Location Covance LaboratoriesInc. 3301 Kinsman Boulevard Madison, Wisconsin 53704 Covance 6329-231 Page 3 Mailing Address: PO Box 7545-2595 Madison, Wisconsin 53707-7545 Study Director Peter J.Thomford, PhD Covance LaboratoriesInc.

BIODEGRADATION (ABS/LAS Shake CultureTest) TEST SUBSTANCE Identity:Perfluorooctanoaiccid,ammonium saltm;ay alsobe referretdo as PFOA ammonium saltA,mmonium perfluorooctanoatPeF,O, FC-116,FC-126,FC-169,orFC-143.

ACUTE TOXICITY TO FISH (RAINBOW TROUT) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component ofFC-203. (1Octanesulfonicacid)(CAS # 2795-39-3).

MICROBICS MICROTOX@ TOXICITY TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfoniaccid)(CAS # 2795-39-3).

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, oras a component of FX-3103. (1Octanesulfoniaccid)(CAS # 2795-39-3).

ATTACHMENT TO LETTER TO C.AUER DATED AUGUST 31,2000 SUPPLEMENTAL SUBMISSION N-ETFOSE Icohol ylperfluorooctanseulfonamidoethanol AcuteToxicit 1.

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