Document v1BLd8bKdLgmdQJG72vYy0Ovw
MICROBICS MICROTOX@ TOXICITY TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber isnotnoted. The testsample is FC-603EF. Currentinformationindicatesitisa mixtureof1.30% PFOS, 10% diethyleneglycolbutylether,54.15% water,22.5% ethyleneglycol,2.6% propanesultonefoamer,5% Alkylethersulfate0,.1% N-(3Chloroallyl)hexaminiucmhloride0,.9% Xanthan gum, 0.9% starch,1.0% triethanolaminea,nd 0.05% tolytldazole.
The followingsummafy appliesa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the tluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotox@ "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1996 Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@) Test organism source: Microbics'Reagent. Test conditions:
Diluent: MilliporMeilli-QTM water Test temperature: Not noted. Stock and testsolutionpreparation: A 500 mg/L primarystock solutionwas pre:)aredby dissolving50 mg testsubstance in100 mL MilliporMeilli_QTwEater. The pH ofthestocksolutionwas 6.3. An osmotic adjustmentwas made using NaCl dissolvedin10 mL stock solution.Appearance oftestsolutionswas noted as "clearand colodess".A"lltestsolutionswere made by proportionadlilutions..
Exposure vessels: Not given. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS
Nominal concentrations:Blank control3,1.25,62.5,125, and 250 mg/L
Element value:
5-minute EC50 = 217 (215 - 219) mg/L 15-minuteEC50 = 146 (143 - 149) mg/L 30-minuteEC50 = 124 (118 - 131) mg/L
Element valuesbased on nominal concentrations.
Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 30-minute EC50 forPhotobacterium phosphoreum was determined tobe 124 mg/L witha 95% confidenceintervaolf 118 to 131 mg/L.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking= 2.Thisstudy meets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportionin solution.
REFERENCES
This studywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number P2581, 1996.
OTHER
Last changed: 6/26/00.
05/26/2000
ENVIRONMENTAL LABORATORY STATUS REPORT
LAB REQUEST NO. P2581
REQUESTOR NAME: J MOHS-DAVIS PACE/SHARI LOUSHIN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 08/14/1996 DESC: FC-603EF LIGHT WATER(TM) ATC 3%,AR-AFFF
CONTRACT LAB(S): PACE ASCI
EXP COMP DATE: 11/18/1996 DATE COMPLETED: 01/28/1997 PROJECT LEAD: SA BEACH PHONE NO: 651-778-7452 3M FAX NO: 651-778-6176
236-GL-04; 7-7490.
Standard Testing. Formulation is on document #07-7297-0. Results from Microtox Toxicity Test:
Test Conc. mg/L 31.25
62.5
125 250
Percent Light Loss
5-min Exp.
15-min Exp.
5.1
9.6
14
20
35
45
54
66
30-min Exp. 13
21
50 72
EC50 95% C.I.
220 (215 - 219)
146 (143 - 149)
124 (118 - 131)
sab 10/31/96
Results from Activated Sludge RespirationInhibitionTest:
Test Conc. mg/L
10 100 1000 1000 (dup)
Percent Inhibitionin RespirationRate
30-min Exp.
3-hr Exp.
0
0
0
0
0
0
0
0
EC50
> 1000
> 1000
sab 10/31/96
Results from Daphnia magna acute toxicity testing:
Test mg/L 62.5
125
250
500 1000
Conc.
Percent Immobilization
24-hr Exp. 48-hr Exp.
0
0
0
0
0
0
15
60
25
85
EC50 95% C.I.
> 1000 -
480 (380 - 610)
NOEC*
250
No Observed Effect Concentration
Results from toxicity test with Selenastrumcapricornutum(green algae):
Test Conc.
Percent Inhibition in Average Specific Growth Rate
mg/L
24-hrs Exp.
48-hrs Exp.
72-hrs Exp.
96-hrs Exp.
05/26/2000
ENVIRONMENTAL LABORATORY STATUS REPORT
2
LAB REQUEST NO. P2581
COMMENTS CONTINUED
31.25 62.5 125 250 500
0
12
0
3
1
12
1
7
53
15
4
9
56
40
41
36
82
79
91
85
EC50 95% C.I.
170 (150 - 200)
290 (250 - 330)
275 (250 - 300)
290 (265 - 330)
NOEC*
125
No Observed Effect Concentration.
Effects were determined to be algistatic.
sab 12/2/96
Results from Fathead Minnow acute toxicity test:
Test chambers aerated with gentle, single-bubble aeration due to loss of dissolved oxygen observed in range-finder studies.
Test mg/L 130 216 360 600
Conc.
24-hrs 0 0 0 0
Percent Mortality Exp. 48-hrs Exp.
0 0 0 0
72-hrs 5 0 0 5
Exp.
96-hrs Exp. 20 5 10 15
LC50
> 1000
>1000
> 1000
> 1000
sab 1/27/97
--------------------------------------------------------------------------------
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
--------------------------------------------------------------------------------
1
FC-603EF
AMBER LIQUID
PACE ASCI ASCI ASCI
2
P-CBOD-05 P-CBOD-10 P-CBOD-20 P-CBOD-28 COD IC50-ALGAE BOD-REVIEW 48-HR-EC50 96-HR-LC50 MICROTOX SLUDG-RESP
BOD-05 (CARBONACEOUS) BOD-10 (CARBONACEOUS) BOD-20 (CARBONACEOUS) BOD-28 (CARBONACEOUS) *COD *ALGAE ASSAY - IC50 BOD TESTING REVIEW *DAPHNIA ACUTE TEST *FISH ACUTE TEST - 96 MICROTOX - EC50 SLUDGE RESPIRATION -
HOUR EC50
216000 MG/KG 376000 MG/KG 446000 MG/KG 602000 MG/KG 740000 MG/KG 290 MG/L COMPLETE 480 MG/L >1000 MG/L 124 MG/L >1000 MG/L
DUP #1
------------
2500 (265 - 330)
(380 - 610)
(118 - 131)
P-CBOD-05
BOD-05 (CARBONACEOUS) CONTRACT LAB DATA
110000 MG/KG
05/26/2000
ENVIRONMENTAL LABORATORY STATUS REPORT
3
LAB REQUEST NO. P2581
CONTINUED
--------------------------------------------------------------------------------
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
--------------------------------------------------------------------------------
------------
2
DUP #1
P-CBOD-10 P-CBOD-20 P-CBOD-28
BOD-10 BOD-20 BOD-28
(CARBONACEOUS) (CARBONACEOUS) (CARBONACEOUS)
326000 439000 318000
MG/KG MG/KG MG/KG
LNVIRONUZ
RATO RY-.@'MCROTOX
PREP SHEET, L
TEST S
epara no
FimIDifulidii'mVo
IC-@
.'2. 7-Sr
'ell
FinalConcennnon.df Stoc-
2.pH Adifu
.,p 0
nnearance.,
o
MICROTOX.DATA REPORT-
t'-7,i.Tme
-minu*teis
---I-O-/-I-T-----. .....
niiol 94.25/ 92.18 'Contro1 .,95.09/ 94 15,
mo N(I 0 ty
AdJ-"'tmen -N CR@9
9780 4 0 990
90.37/ 84..73:,,
4L
90.97/ 84.58--.
3
88.85/ 76.45
411.1.1. 89.29/,74 60
91 .061 57.81
,f
6
@91.22/ 59. 59
7.
91.42/ 40. 9 9
01.88/ 4 1 2 8
0
,COCORRP.CTION FACT B
1"'z@U"d 'foi,-calculations
0
odo ooci
@@@.@"250@'dOoo 5 ;.bbon
0-058 -o':144
178 0.550
6 1)6
9 0,
-'InVa'liddat"a; or
6 .7934-m G/L '(95% CC)NFIDEN,CE@,,,iiaL4ujsZ4I
lp
DATA, REK)ORT p
NUMBEA., , !
0/1
"','-Control :@'59 0 971
a Z.,-
82'.31.1 69,
9 CO "CCOoCORORR
inot id AdJust men t: N a Ul
-CONC ----------
CR/G A MMA
1 0184' 1.007 7
31.2500 31.2500 62.5000"62.500( 125. Of)00, 126,00
250- 0 25@O 'OOiDo.
0. 11 0.102 0 245 0.249:
0,'82 '07
1.976
9.2-
ci 2-
)k,
EC50-i", 67
ARGE.- 143- 3.3,1)3TO ,148.13
MICROTI)X DA'RA REPORT
0
Basic Test
FILE P2581.K30
10-22-96
TKS
Test Time: 30 minutes
Osmt-),ticAdjustment,:NaC!
14U.MBER --------
Control CC)ILtrol
IO/IT ------------
94,25/101.5@,
95.09/ 9 9 .3
COINC.
).0 f)
CR/(7AM@IA
.0771 1$ 1.044;3
9 (1@1-'7i / 8 3. 7 (-j
2
90.97/ 84.39
3
88.91,/ 7 5. 4'@,
4
89.29/ 7,1.
5
6
91
47 .J4
7
Ql.42/ 27.8:i
-ii@2500 31.2500 62.500i)
50 ok)
@.i).0000 0 .0 a ()ii
@,50.0i)00
0. I,IE; 0.14 4 0 .2!-, (i
-,.()1931 2-. 4 z35
.535
Co(7;,F.',@FCTIC)NFACTOR
1
c a 0 u I fit i
EC50 124.3101 MC@/L
9 5 l@
F tl'
1.17.5475 T(,'
Ec@ 1@IATE: TIM
it,