Document 4vKzDgKJ2mK5D03m7nV9rgRxx

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, oras a component of FX-3103. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFX-3103, 3%. Currentinformatioinndicatesitisa mixtureof0.96% PFOS, 20% diethyleneglycolbutylether,50.56% water,15% ethyleneglycol,4.32% sodium octylsulfate6,.0% polyoxyethylenemonooetylphenylether,2.5% propane sultonefoamer,0.5% sodium nitrate0,.06% sodium laurylsulfate, and 0.1% benzotriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentradons of impudties. Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute static-renewarlange-finder GLP: No Year completed: 1985 Species: Pimephalespromelas Supplier:Commerical SupplierB,rady,NE. Analyticalmonitoring:Temperature,pH, and dissolvedoxygen. Exposure period: 96-hours Statisticamlethods: LC5ovalueswere calculateudsinglogistriecgression methods (probiatnalysis). Test fishage: Juveniles Length and weight: Average length 41 mm Loading: 0.67g/L Average weight 0.50g (wet) Pretreatment:25 mg/L TetracyclinHeCI Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutionwater chemistry:Not given. Lighting:Notgiven. Stock and testsolutionpreparation:The testmateriawlas added directly tothedilutonwateron a weight/volurnbeasis. Concentrations dosing rate:Solutionrenewal duringthestudy. Stabilitoyf the testchemical solutions: Not noted. Exposure vessels: 4 L glassbeakers containing3 L testsolution. Number of replicates:1 forexposure concentrations2,forblankcontrol. Number of fishper replicate:4. Number ofconcentrationfsi:vepiusa blankcontrol Waterchemistryduringthestudy: pH range(0-96hours): 7.6- 7.8(contreoxlposure) 7.7- 7.8(1000 mg/L exposure) Temperature range (0-96hours): 19 - 20 OC Dissolved oxygen range (0-96hours): 7.1- 9.3mg/L (controelxposure) 5.9- 9.3mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,1,10, 100, 500, and 1000 mg/L Element values: 96-hourLC5o = >1000 mg/L Element values based on nominalconcentrations Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hourLC50 forfatheadminnow was determined tobe > 1000 nig/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:Jimischranking3. The studywas a range-findewrithlimited number oforganisms exposed ateach concentrationT.here isa lackof charactedzabonofthetestsample. Test solutionslackanalyticaclonfirmation ofthe amount offluerochemicalproportioninthe solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Cl 111-1,1985. OTHER Last changed: 6/26/00 lpe Tes EXPLORATORY (RANGE-FINnER) LR Material Tested_,,@ IDt ast Org Source Date Pacld Batch ,ig.Wtt:-:@@g,ram Avg. Size :ZLc:t@ No. Ctrganismsper dose Ioading Factor l@7, 9/1 ish Disea ast Solution Volume @_l Litwo/All-Gams TW* Tank Dkwmions: 4 -1iter Pyrex@ blass I)eaker imestarted Z@@@Mte sta@ Zg-Z @Date Cmpleted ilutim Water Carbon-filteredwell water (TeM. C. s,Do0. s 'WI (95% Confidenoe Limits) .,Uu S.Lgna rw* Initial7btal 24 Hours 48 Hours 72 Hours 96 Hours 140. Conc. Amount Thtal No. D.O. 7btal No. pH D.O. !rbtalNo. pH D.O. Tbtal No. pH D.O. mg/l Added Dead ppn Dead Dead ppm Dead PPM Cbntiml in C)o Test Tenip, OC Initials& Date ?Inl wondary Effeqts (otherthan Morta3.ity): rank No. mtroi Symptoms or Reactions 24-4ir. C BC50 Tbtal Number Affected 48-Hr. 72-Hr. 1-2- 96-Hr. . 0-moo CHEKICAL CONCENTRATION ENVIRONMENTAL LABORATORY .TABLE I* SUMMARY OF ACUTE TOXICITY OF TO'FATFMAD MINNOW UNDML--':@,@?@,W A,4M-WI,ZXPOSUItE CONDITIONS 24-HR. PERCENT MORTALITY 48-HRO 72-HR. CONTROL esnr) 0 C) C) 0 0 (9 0 0 C) 0 c L4c (95% CONFIDENCE 50 LIMITS) (2) >/000 -),/Ooo .@/OOU (1) Data were averages of two replications for each concentration with ten (10) fish per replicate. A total of twenty fish were used per concentration. (2) LC values were calculated using logistic regression aethods (Probit Analysis). 50