Document 6R2oarbkg9Yne1gaNKdm1MQY6
AR226-3089
HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE
H-23005: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna
PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed
test concentrations and data measurements at predetermined intervals.
GENERAL INFORMATION
WORK REQUEST NUMBER: HASKELL NUMBER: TEST CODE: MATERIAL TESTED: SYNONYMS/CODES:
SPONSOR: NOTEBOOK ID: SUBMITTER'S NOTEBOOK NUMBER: COMPOSITION:
None available
CONTAMINANTS:
CAS REGISTRY: None available PURITY: Not supplied by sponsor.
TEST CONDITIONS
Nominal concentrations tested: 0,0.5, 1.0, 50, 500, and 5000 mg/L.
Nominal Concentration
(mg/L)
0
0.5 1.0 50 500 5000
Test Initiation (O hours)
Dissolved
Oxygen
(mg/L)
At
Bt
pH
At
Bt
8.8
8.7
73
73
8.8
8.6
7.3
7.3
8.6
8.6
7.3
7.2
8.5
8.6
6.7
6.6
8.6
8.6
6.1
6.1
8.6
8.5
5.2
5.2
Test Completion (48 hours)
DisscDived
Oxyfgen
(m!/L)
At
Bt
pH
At
Bt
9.6
9.6
7.5
7.5
9.6
9.5
7.5
7.5
9.6
9.5
7.5
7.5
9.7
9.6
7.5
7.5
9.7
9.6
7.5
7.5
8.7
8.7a
5.5"
5.5"
IA and B represent replicate test chambers within a dose level concentration.
a Measurement taken on day 1 (24 hours) due to total immobility.
Company Sanitized. Does not contain TSCA C%
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DuPontHL-1998-01379
H-23005: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna, CONTINUED
RESULTS
Based on visual observations, the water control solution and the 0.5,1.0,50, and 500 mg/L test solutions all were clear with no color. The 5000 mg/L test solution was cloudy yellow-white. After 24 and 48 hours undeserved test substance was found at the bottom of all test vessels of 500 and 5000 mg/L test solutions. Immobilities were 0,0,0, 0,70, and 100% in the 0,0.5,1.0,50,500 and 5000 mg/L, respectively. The 48-hour ECso, based on nominal concentrations and immobility, was between 50 and 500 mg/L. All water quality parameters were within acceptable limits except the pH of 5000 mg/L was low.
CONCLUSIONS
H-23005 exhibited slight toxicity in a 48-hour, unaerated, static acute test using Daphnia magna.
WORK BY:
) ^ -- < w ^ J&mesW.Wetzel.B.S.
^dcicology Associate
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APPROVED BY:
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Paul W. Grube, B.A. Study Director
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DISTRIBUTION:
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