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BOD/COD resultstorFC-99 (DEA saltotPFOS) BIODEGRADATION TEST SUBSTANCE Identity:PerfluorooctanesulfonatDeE,A salt;may alsobe referredto as PFOS DEA salt,FC-99, or 3M Sample No. 2. (1-Octanesulfonic acid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafcloumoprdo.-, with2,2'-iminobis[ethan(o1l:]1)C.AS # 70225-14-8) Remarks: Testsample isa mixtureofthe testsubstanceinwater (approximately25% testsubstance and 75% water).Allvaluesreported relatetothismixture.No calculationwsere made to adjustforthe actual concentrationofthe testsubstance inthetestsample. METHOD Method: Not noted. Type: Aerobic GLP: No Year completed: 1979 Contact time:20 days lnnoculum: Sludge collectefdrom an aerationbasinatthe municipal waste treatmentplantinSt.Paul,Minnesota. RESULTS 5-day BOD: 20-day BOD: COD: 44,000 mg/kg 82,000 mg/kg 78,200 mg/kg Remarks: This materialhas a perfluorinatemdoietyand a nonfluorinatemdoiety. Currentknowledge ofthese chemistriessuggests that allbiodegradationindicatedinthisstudy islikeldyue to reductionofthe non-fluodnatedmoiety,2,2'-iminobis[ethanol]. Testingwas conducted on the "as-sold"mixtureofthe subjectmaterialin water (-24.5% subjectmaterial)T.he values reportedapplytothat mixtureand notdirectltyothe subjectmaterial. CONCLUSIONS This testingindicatedthatthe 2,2'-iminobis[ethanmooli]ety islikeltyo be biodegraded inaerobicenvironments capable ofsupporting biodegradation.This study cannot ruleout the possibilithyatconditions could be found thatwould allowforbiodegradationofthefluorochemical moietyn,orcoulidtexcludethepossibiltihtayta microorganiscmould eventuallybe enrichedthatcould metabolicallaylterthischemical. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 333.31, St.Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking3. This study lackssufficient documentation,characterizatioonfthe testsubstance puritya,nd analyticamleasurement oftestconcentrations. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory,Lab Request number 48955, 6/8/79. OTHER Last changed: 5/2/00 Form 10620-5-PVVO ot: LAD R.EGUEST BOOK - 42-5W PRODUCT ECOLOGICAL ASSESSMENT REQUEST FOR LABORATORY WORK Ref: ENVIR4SSESS. ING. DIVISI N -FRODUCT: 'FC, -It (4--'7Y071 - 2 LAB REQUEST NO. ? qp REQUESTER LABORATORY colt,17T Name: 4*/, RequestDate: -Cl&ft2'7 ProjecNto.:!%*7CP*lalt0, 12 Code No.: Sample Date: SIYI?L Phone No. Prob. No. Date Needed: Description: Purpose of @ests:-P#X c 0-ce $L 4-SAtO Date ReceivedSample. Analyst Date Completed: Hours Spent.e, 14@T- !2 1"I-LC'0p- Y_B_ -0179cr P7 PleaseCallRequester IfDelaysAre Encountered. PHYSICAL & CHEMICAL PROPERTIES Hat ofCombustion Cal/G (STU/LbJ Density Ash Other -T WATER POLLUTION -PH COD W-A -801) 5 Vi 800,Ultimate TOC -GC Oxygen Uptake Dshvdr2rnase ActivitY(TTCI Other i@&[) pe odo e2oe V/ >1.o 2-2-900 -71x9:Qo L,AC-714rt Ae&uc@2 > AIS-1- 6iiL'A_-"p 7-rA-- L4 BIOASSAY SpecifyOrganism and Method: LEACHATE FROM LEACHING PH COD BOOS 800U,i*,invis TOC GC TEST Other OTHER Bioaccumulation Persistence Dearsdation Pro-duc-ts yridat- si)-a v Af4 61& Wt%i"-ft~" to itumemer CM-0- - it e%"t- 9 91- Fifti-tUL"V inmu_ Sekiwmd -ftowrn m Pl-um~ ULTiMA6 iE. &@D rclavLe,omt4co-TAL " (,"rjRATORY CA@$mPoLtgD APALYST DA-RE DARTA- 56(ef,-r %4^PLE -0/0 90 .160 BODS- i a9 > oo o *Sol>is (DD d> lot0*0.0 (0/ > #q ooo f4t I)bo 1.50DZO =54 /*.a ooO -77/000(l) -7 92-looo (ZCAIcAjie,.25LO'rE -T io -7@A z444t.W.-0.4-- -U@@ AvAA4L *a bo 444.@ z A, > bo &i.AA--) lit vo AASO&OP FOR"164akwI rwo BottleNo. SempleSize ENVIRONMENTAL LABORATORY BW WORK SHEET Blanks: Domeriptim Fe -!zt) tvD-.040 Doo lpin Date:;S'z DilutWiaotner: Seed: I Sk.Avg. .24A SDay SOD Avg. -7416- (04 All to 2.s.a40-so ODD Iq C)OO At A AL A ponti. 104ke-4 rwo Bottle NO. 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D"crlptim 106% D Depladon 13 -Day BOD Avg. r.s*o I-ssi-io of57T I,iD l,25- 0-030' 94"A,&,,, 71q 419 -.3,,-2)o,4:r 91 c-- v 1-7 H2.0) /.(.a -7 "I@ jqob dgl-,p 0 Yo -z.clo %,go 44, gspi I s --aro Z!50 7SO A A (Z 00% .Clio4a*-4 rwo ttleNo. 2-3 SBMPIO Size 213q ENVIRONMENTAL SOD WOOK LABORATORY SHEET Blanks: Onwotion 101 D.O. Dsplatim A 41 1,(.6. 7,10 A B4*^A,&., -5Lgo (oIr@r, :&go 41c" Page No. Date.. DilutionWater: Seed: 3k. Avg. Day BOO Avg. 719 91 /3 /7 H2.12 31'70- 97 IA/ -I-Al 13 '50- @POb S&M, ISV-MG " V/5-omt-4 C9.@@ &tA. v S.e0l 2-(.o0 %,go wt, .001L1/