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"ARRKG -- 137/ ANALYTICAL METHOD VALIDATION FOR THE DETERMINATION OF PLANT TISSUES PERFLUOROOCTANESULFONATE, POTASSIUM SALT (PFOS) IN `WILDLIFE INTERNATIONAL, LTD.

caerinOf Ip wSeY e siaF00E0 HO-020A0R-16263-703545 RECEIVED OPPT ceIC WONAR 20 Aif11: 43 March 17, 2000 (DAotctun:meSnetctPiroonce8s(s)inCgooCrednitnearto(r7)407) obPT Room G-099 US EPA 401 M Street, SW.

bn Te wt oe an al } `DuPont HaskellLaboratory Disecior,Applied Tox . benTuxicology DuPoat Haskell Laboratory v P.0.Box 50 Elkton Road Newark,Delaware (9714-0050 : Phioge: (302) 366-5259 OS a v Hai kae aa rr ocr 40 220-1559 * Dumcas Control Office (7407) `Room G99 East Tower ATTN: FYT 4OUf0Sf1.icMEevoSftirrPeoeotln,lumSteiWon.ntPPrreovteencttiioonnaAngdeTnocxyics `Washington, D.C. 20460-0001 .

TTT a Specialy Materials 3SuMPCaeunlteMrN 55144-1000 S17 0 Ae226-1717 May 11,2004 TSCA Non-Confidential Information Center (7407T) Office of Pollution Prevention and Toxics (OPPT) EDoPcAkeWteAstR-B2u2il6ding, Room B-146 1301 Constitution Ave., N.W.

STABILITY IN WATER (PHOTOLYSIS) TEST SUBSTANCE Identity: Perfluorooctanesulfonate-potassium salt.

Estrous Cycling and Natural Delivery/Litter Observation Data F1 Estrous Cycling Data, From Data Analysis Presented at EPA on August 30, 2002 a APFO Dose Group (mg/kg/day) 0 1 3 10 30 All 4-Day Cycles 3-5 Day Cycles 14/30 24/30 19/29 26/29 9/30 21/30 15/29 25/29 15/30 27/30 Cycle Length (Days) 2 3 4 5 6 7 8 Mean Cycle Length (Days) S.D. 1 2 75 18 5 1 2 4.3 + 0.8 Incidence of Cycle Length Per Group 0 1 80 16 2 1 0 4.2 0.5 5 7 57 32 5 0 1 4.3 + 0.9 2 5 84 14 3 0 0 4.1 0.6 5 5 86 17 0 0 2 4.1 0.8 Aberrant Cycles b Parental > 4 Days Diest. 2 1 1 4 1 > 3 Days Est. 17 13 15 16 13 F1 > 4 Days Diest. 5 3 3 5 3 > 3 Days Est. 25 25 29 11 14 Distribution of Cycle Status at Start of 21-Day Evaluation Period, F1 P 1 4 1 2 5 E 8 10 11 14 11 M 6 2 2 0 2 D 15 13 16 13 12 Distribution Rats in Estrous at End of 21-Day Evaluation Period, F1 End in Estrous 13 17 18 19 19 Mean Implantations, Liveborn, and Stillborn S.D. per Litter by Group" Parental Litters Implantations Livebom StiUbom 15.3 1.9 14.2 _: 2.2 0.1 0.3 15.6 2.1 14.2 + 2.6 15.2 2.3 13.4 3.6 0.4 1.4 0.2 0.4 F1 Litters 15.6 + 2.0 13.8 3.0 0.1 0.4 15.3 2.0 13.4 3.3 0.2 0.6 Implantations Livebom Stillborn 14.8 3.0 13.6 + 2.9 0.2 0.5 15.8 2.1 13.7 2.4 0.2 0.5 14.7 2.8 13.8 3.0 0.2 0.6 15.0 2.9 13.5 3.1 0.2 0.5 14.5 2.9 13.3 2.8 0.1 0.2 a Original analysis counted cycles from the day following estrous (or proestrous if diestrus followed proestrous without observation of estrous) to the day following the end of the next estrous.

Extraction of Potassium Perfluorooctanesulfonate from Red Blood Cells and Serum for Analysis Using HPLC-Electrospray/Mass Spectrometry DATA REQUIREMENTS Analytical Method Requirements Sean Gallagher ANALYTICAL REPORT DATE June 2, 2003 PERFORMING LABORATORY / TESTING FACILITY 30E3x8ygReesneRaerscehaDrrcihve State College, PA 16801 Phone: 814-272-1039 STUDY SPONSOR 3M Environmental Technology and Safety Services Building 2-3E-09 PO Box 33331 St.

MIN 3131023622 PERFLUOROOCTANESULFONYL FLUORIDE (POSF; T-7661.4) TOXICITY STUDY BY INHALATION ADMINISTRATION TO CD RATS FOR 13 WEEKS FOLLOWED BY A 4 WEEK RECOVERY PERIOD Volume 1 Sponsor. 3M Center, 3M Corporate Toxicology, Building 220-2602, StPaul, MN 55133-3220, USA.

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AR226-2286 Frank D Smith "^Sy 05/13/2002 08.-25 AM To: cc: Subject: Catherine A Barton/ABDuPont DuPont Kenneth N Wood/AE/DuPont@DuPont, James F Riley/CL/DuPonlQDuPonl.

AR226-2471 DiiPofti WsshiBpon Woiks P. 0.

AR226-3211 TRADE SECRET Study Title H-23961: Biopersistence Screening 10-Dose Oral Gavage Study in Rats Supplement No. 1 Laboratory Project ID: DuPont-2923 AUTHOR: Carol Finlay, B.A.