REPORT 7/15 Occurrence and use of highly fluorinated substances and alternatives Report from a government assignment kemikalieinspektionen.se KEMI Swedish Chemicals Agency The Swedish Chemicals Agency is supervisory authority under the Government.

AR226-2776 (SS INHALATION: EMBRYO-FETAL TOXICITY AND TERATOGENICITY STUDY IN THE RAT HASKELL LABORATORY REPORT NO. 881-81 Copies to: Company Sanitized.

AR226-2852 PERSOWAL AND CONFIDENTIAL Copies l:o:l E.

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AR226-3339 --------------------^^ Roy S Thompson (AstraZeaeca, Brixham, UK) Haria Colombo Watze de Wolf (Seh'ay Sotexis, BaHate, Stely) (OuPoBt, Mechelt'n, Belgiaaa) David G Fanrar Robert A Hoke Jacques L'Haridon (laeos C&ior; Ruacorn, UK) (DaPont, Newarfe, BE, USA) (CST, Evreux, Fraace) for.

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CENTRE FOR XENOBIOTIC RESEARCH UniversityofDundee, BiomedicalResearch Centre,Level5,NinewellsHospitaland Medical School,Dundee, DD1 9SY Study Report Determinationof cyanideinsensitivPeaimitoyi-CoA oxidationinsamples from 3M Environmental Laboratory - Covance Studies6329-183 and 6329-212 Study Number XR0108 Author: Approved: t.M.Elcom,be C.R.EL .e- XR0108 PaLyeI of 3 Date: I Introduction The purposeofthisstudywas toinvestigattehelevelsof PCOA oxidationinthefrozen liversamplesprovidedby 3M EnvironmentalLaboratoryfrom Covance studie6s329-183 and 6329-212. 2 TestSubstance 6329-183:PFOS, 20ppm, administeredby Covance untilweek 4. 6329-212:ETFOSE-OK 100 and 300ppm, administerebdy Covance untilweek 4. 3 TestingFacility CentreforXenobioticResearch,UniversityofDundee, BiomedicalResearchCentre, Level5,NinewelisHospitaland MedicalSchool,Dundee,DDI 9SY, UK. 4 Homogenste Preparation 4.1 Reagents SET buffer0:.25M Sucrose,5mM EDTA and 20mM Tris-HCI,pH 7.4 4.2 Instruments Potter-Elvehjehmomogeniser 4.3 Method Frozen liversampleswere thawed on ice,blottedand weighed.A 25% homogenate was preparedinicecoldSET usinga Potter-Elvehjehmomogeniser(6passes). 5 Determinationof Cyanide-in.sensitPiavlemitoyi-CoOxidation The 25% homogenate liversampleswere dilutedI partin 10 partswithicecoldSET.

PerfluorosulfonamiMdeetabolisminRatvs..Hum-an--Heya R C H CH S 0 -2N -%.

TABLE I Sectors and (sub)uses Table 1.

-4 HAZLOTON L"BDPffDRIES ANFERICA INC Chemica&l BioMedicaSlcienceDsivision @'N@iol orK.I-NSsMoAN BLVD. - P.O.BOX 7545 * MADISON, WISCONSIN 63707 PHONE (6082)41-4471* TLX 703956HAZRAL MDS UD FINAL REPORT ROGER PERKINS MINNESOTA MINING & TOXICOLOGY ST.