Document jLaBrwgd7YNmYQz8rkBXymNy

AR226-2892 E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Newark, Delaware lS7l. Chemicals fi Pigments Dept. Jackson Laboratory PRIMARY SKIN IRRITATION AND SENSITIZATION TEST ON GUINEA PIGS Procedure; A range-finding study was conducted with 3 male albino guinea pigs to test for skin irritation potential This material was a skin irritant as low ae 25% in distilled water; it did not irritate the skin at 5%.t The primary irritation test was conducted on 10 unexposed guinea pigs by applying and lightly rubbing in 0.05 ml of a 25% and 2.5% solution of the test material in distilled water on shaved, intact shoulder skin. _ The induction phase for sensitization was a series of A sacral intradermal injections of 0.1 nl jf a 1.0% solution in distilled water, 1 each week begi.aing 2 days the test for primary irritation. After a 14-day rest pe*.*eA,^Ve test guinea pigs were challenged for sensitization by applying and lightly rubbing in 0.05 ml of a 25% and a 2.5% solution of test material In disf'.led water on shaved, intact shoulder skin. At the same time 10 unexposed guinea pigs (controls) of the same age received identical topical applications. 1 f. Does not contain TSC CBI Results: Reactions** on Intact Guinea Pig Skin ________ . .0t Animals________ Concrol A.i.'ials_____ (10 Animals - In. Avg. Mt. " 550 g (10 Animals ~ In. Avg. Wt. 545 g 10 Animals Final Avg. Wt. * 735 g) 10 Animals Final Avg. Wt. 749 g) Concentration in distilled water 252 2.52 Primary Irritation Test * 24 Hours 10 Neg. 10 Neg. 252 2.52 - 4t> Hours 7+, 3 Neg. 10 Neg. Challenge Test - 24 Hours 1+, 9 Neg. 10 Neg. 1+, 9 Neg. 10 Neg. - 48 Hours 6+, 4 Neg. 10 Neg. 6-r, 4 Meg. 10 Neg. Sensitization Response Ratio*-* at 25% Concentration ~ Q/lQ mild to no skin Irritation when tested as 'a 52 s ^ R t S n M l ^ d i s t i l l e d water on the 9haved intact skin of male albino guinea pigs. A 2.52 solution in distilled water produced no skin irritation. No sensitization was observed at challenge. No unusual dermatitis hazard is expected from handling this material if good industrial hygiene practices are employed, although higher concentrations (502) can produce severe irritation. * Composition Synonyms; CAS Registry Number; 2- - not contain TSCA CBI DOM t Range Finding Results: Test Material 2 (w/v) 100 (As received) 50 25 5 Reaction on Intact Guinea Pig Skin 2 Necrosis, 1 Moderate 2 Necrosis, 1 Moderate 1 Moderate, 1 Mild, 1 Neg. 3 Neg. ** Reaction Code: +, ++, -H+ mild, moderate, strong erythema; ++++ " erythema plus edema, * H ( necrosis. *** Sensitization is defined as a significant score increase at challenge over the response expected from the same amount applied initially or on the concurren", controls. Report by: John . Ben Technician Approved by: --y Gerald L. Kennedy -- Chief, Acute Investigations Section JEH;Jrg Study Director: J. L. Dashiell Date Issued: February 17 l!*8l Report No. 71-81 - 3- Company Sanitized. Doot not contain TSCACBI