Document wqYzOMadb2587VBQO01Ro2mJ3
AR226-2905
FOR DU PONT USE ONLY
E. I . du Pont de Nemours end Company H ask ell Laboratory fo r Toxicology and In d u s tr ia l Medicine
Elkton Road, Newark, Delaware 19711
Haskell Lab.
ORAjL LD50 TEST IN RATS'
Procedure; The o r a l LD50
d is t ille d water
as the v e h ic le was found to be ,196 m g / k g T o evalu ate the
e f f e c t , i f an y, o f the v e h ic le jused, Che te s t m a t e r i a l ,^ a suspension in
corn o i l , was adm inistered by ih tr a g a s tr ic Incubation in s in g le doses to 6
groups o f 10 young adult CrliCD* male r a t s . The su rvivin g r a ts were weighed
and observed during a 14-day recovery period and Chen s a c r ific e d . The LD50
valu e was c a lc u la te d from the m o rta lity data using the method o f D . J .
F in n e y .* *
`
Results:
Dose
Average Body Suspension Average M o rta lity
(mg/kg) Height (g)
(*>
Doss (mL)
R a tio
LD50***
3,700
257
25
3.81
10/10
916 mg/kg
2,000
247
25 1.98 10/10
1,500
262
25 1.57 8/10
1,000
245
23 1.07 8/10
850 259
19 1.16
4/10
500 245
11 1.11 0/10
Company S r i * * "
TSC* ^
C lin ica l Signs:
Stain ed and/or wet perinjeal area and weight lo ss at a l l le v e ls te s te d . Diarrhea a t a l l le v e ls except 500 mg/kg.
Stain ed fa c e a t a l l le v e ls excjept S00 and 850 mg/kg. Weakness a t a l l le v e ls except 1500 and 3,700 mg/kg. Tremors a t 1,500 and 2,000 mg/{kg.
Chromodacryorrhea a t 1,000 and;' 2,000 mg/kg. A l l deaths occurred w ithin 5 djeys a ft e r dosing.
Summary;
__ [la s l i g h t l y to x ic when adm inistered o r a lly to
young a d u lt male CrlrCD** ratsTTn s in g le doses; I t s LD50 i s 916 mg/kg o f body
w eigh t. C lin ic a l sign s observed at most dose le v e ls included: stained
and/or wet p e rin e a l a r e a , weight l o s s , d ia rrh e a , stain ed fa ce and weakness.
A l l deaths occurred w ith in 5 days a ft e r dosin g. The LD50 in corn o i l was
approxim ately 1/2 ehat determijned when the chemical was adm inistered in
d is tille d water.
!
* Pu r i t y ; Contaminants: Synonym:
** F in n e y , D. J . , P ro b it A n a ly s is , 3rd E d ., 1971, Cambridge U n iv e rsity
Press.
;
f * * * 952 Confidence L im its:
low er: 746 mg/kg
'Upper: 1,086 mg/kg Slope: 6.5
Report by:
1 TL.onn UH4inncr kle Technician
Reviewed by:
Study Director
Approved by:
Jt J -X
JL. \ _______
Gerald L . Kennedy}- A .
C h ie f, Acute Investigationfc Section
LEH/J td/WP:1.8 Date Issued: October^.7, 1981
Report No
Company Sanitized. Does not contain T SC A CBI
