TBCU was not mutagenic in the Ames test (DuPont, 1977).

References DuPont Co., Haskell Laboratory Data: 1976a.

DuPont. has three action levels on problems such as this.

AR226-3111 DuPont-1977 TRADE SECRET Study Title H-23746 and H-23747: Skin Irritation Test in Rabbits Laboratory Project ID: DuPont-1977 A u t h o r : Carol Finlay, B.A.

DuPont wou1~ be interested in the results of these studi{s.

Stahl, DuPont R.

AR226-3111 TRADE SECRET Study Title H-23746 and H-23747: Skin Irritation Test in Rabbits Laboratory Project ID: DuPont-1977 AUTHOR: Carol Finlay, B.A.

DUPONT DE NEMOURS & COMPANY NEWARK, DELAWARE ^Lc ^u^ Acute^ toxicity of 8233 to rainbow troutj (salrno gairdneri).

Of all the unlikely explanations above,the least unlikely is residual FC 143 for whatever) we sell to DuPont to polymerize TFE in Teflon cookware.

DUPONT DE NEMOURS & COMPANY '^gy A cute t o x i c i t y o f 8233 to rainbow Bionom ics, In c , 790 Main S tr e e t Wareham, M a ssa ch u se tts Ju n e , 1973 m S?

The incidence o f nodular hyperplasia in the liver (males: 1/50,0/50,2/50; females: 0/50,0/50, 3/50) was also slightly increased, though not statistically significantly (DuPont, 1987). > A ll o f th e other rem arkable tum or incidence values produced in this study w ere associated w ith endocrine or endocrinersensitive organs (DuPont, 1987): . :: " ?

The tumor incidences were outside the historical control incidence range for Haskell Laboratory, and in addition, age-adjustment statistics also supported the conclusion that the tumor incidences were elevated for the liver, pancreas, and testis (DuPont, 1993).

I , DUPONT DE NEMOURS & COMPANY NEWARK, DELAWARE A cuue\ t o x ic it y o f 8231 to rainbow t r o u t ^(Salmo g a ir d n r i) .

BIOACCUMULATION (ANALYTICAL ONLY) TEST SUBSTANCE Identity:N-ethylperfluorooctanseulfonamidoethanol;may alsobe referredtoas N-ETFOSE Alcoholor FM-3422. (1-Octanesulfonamide,N-ethyl1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-N-(2-hydroxyethyl)-, CAS # 1691-99-2) Remarks: Materialisan off-whitew,axy solidofuncharactedzed purity.

Attain-ooGI BIOACCUMULATION (ANALYTICAL ONLY) TEST SUBSTANCE______________ Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonicadd, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder.