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m ! AR226-2839 BIOASSAY REPORT SUBMITTED TO . .. E . I , DUPONT DE NEMOURS & COMPANY NEWARK, DELAWARE A cuue\ t o x ic it y o f 8231 to rainbow t r o u t ^(Salmo g a ir d n r i) . Bionomics, Inc. 790 Main Stre et Wareham, Massachusetts June, 1973 \ V ' Company-Sanitized. Does .not contain TSC A C S I * p ; i ---i- mm T h is in v e s t ig a t io n was performed a t the a q u a tic to x ic o lo g y la b o rato ry of Bionom ics, I n c ., W are'hW ,;'M assachusetts. The. s u s c e p t ib ilit y of rairibov tro u t (Salmo jg a ir d r e r l) to 8 231 (a l ig h t y e llo w l i q u i d . te ste d a s | ^ | ) was measured in term s o f the median te ie r a n c e l i m i t (T J^ q )'.,. the n o m in a l c o n c e n tra tio n o f the c h e m ic a l in w,ater w hich causes 50 p e rcen t ; respo nse (death) under +1 e 't e s t c o n d itio n s , d u rin g a s p e c if ie d |jj tim e i n t e rr v aall.. TThhee .p re d iiccttiioonn, of a TTLL'vcan '".yviaalluuee, and i t s 7 :.v . '' * ] ' i -M i? SIIco n fid e n ce in t e r v a l, was based on co n v e rsio n of the c o n c e n tra tio n s te ste d and the co rre sp o n d in g observed^jper:ent m o r t a lit ie s to mi lo g s and p ro h it s r e s p e c t iv e ly , and the subsequent m athem atical c a lc u la tio n of a lin e a r; re g re ssio n equation. The t o x ic it y of the ch em ical te ste d 1w qsievaluated under, s t a t ic b io a ssa y IS 3 !;Sv3 g co n d itio n s fo r a 96-hour Jpriod. *11 The t e s t procedures'' f o r ; the'- s t a t ic b io a s s a y s ' are in complete accordance w ith the F ie ', B ioassay Procedure in the 1970 e d it io n o f S ta n d a rd Methe ds (APHA) except :f o r tho se c o n d it io n s "g*: 7 ? d escrib ed below. mm Wm - M 1 ai ViS IS ! Congucgr Sanftfied: tTowrr-'ircnnrft'n TSC CR? is; ; J MalHHMMMHMHMMHMMIa^tfM^HtfM^nliiittBn The rainbow tro u t used jin these t e s t s were obtained from a co m m ercial f is h h'a t c h%e r / in M'*a:.s'V!s.>a/c'h ui;-*se tts and had a .mean w eig h t o f 1 . 3 g and a mean le n g th o f 5 8 `m m . The t e s t p o p u la tio n was h e ld in jthe la b o r a t o r y -h a t c h e r y f a c i l i t i e s f o r a t le a s t 30 days p r io r to t e ^ t i^ g ^ d u r in g t h is p e rio d m o r t a lit y was < 2 % . s t a t ic b ip |p s|a ys were conducted in 5 "g a llo n g la s s v e sse l s h e ld in temperature ; w ater b a th s a t l i e '(7 1.?. 0 ) . The v e ssie isf w=e''r'e' no t'' a1''e' r a tre d*,' . and th e re was a s in g le lin t r o d u c t io n of, th e t e s t compound in t o the t e s t v e s s e ls * " ! A so lv e n t^ 1centro"l* c o n t a in in g the :v . .-;v.^ g re a te s t amount o f is o p ro p y l a lc o h o l conftained in an y t e s t - . -T,;: ': .1' ! "'-. .:./ & A \ f: v e s s e l, was c onduc t ed *~ F is h were a c c X ia p te'd'-Jt'"-"o- thW'-e t e/s.itTfb:'.'iS*w ' .v.vi.' system c o n t a in in g rcon'lls t it u t e d w a te r the standard d ilu e n t f o r a t le a s t 2^ h p u rs^ p r io r to <testing"'. Ten f is h were exposed ; in each t e s t vessel."^ ' The-" r e c o n s t it u t e d 'v /a t e r used in the .t e s t system was prepared by ad d ing the fo llo w in g chem icals to deionized w ater! f e : ... i:- Hardness 38 m g/l mg of s l t / l i t e r w ater ; !\jaHC03 Caso^ MgSO^ TcT ^ S. l t t l 3P. 1 30 2 -, The pH o f' the sta n d a rd d ilu e n t was 7 . 1 / and the m ethyl orange a l k a l i n i t y was 35 ppm as CaCO^. D is s o lv e d oxygen v a lu e s fo r the v a rio u s te s t v e s s e ls ranged from 8.6 i n i t i a l l y to ^ .0 m g /l a t the, end o f the t e s t s . Company Sanitized, o es n coatiun TSCACB) r 4 b 1h L> 1Si pi mi ;w5.is` y-.s'."''*^ """.,."' rage tl.i toft, .. ';;v .rj>v >X-U-%i, . - " ' ii; RESULTS - *' 4 -,.L' .- *1 \ 'V'-/;>'. ' ' r ;'y$ - *;'VOJj$i -/ "M The p red icted T*L presented in Table; p m o rta lity fo r the b io as Moribund f is h g e n e r a lly ' e q u ilib riu m ,' o f observe.d, of exposure; lo st A-,:A:;:Sm|4 *_' ii P il 3 J ^ _" la s ,,, J Ip iH % -fr.^ ^ A IP ! ; s6ffi^|ny Sanitized. Does not contain TSCA CBI SUBMITTED BY ; Bionom ics, In c. 790 Main S tre e t Wareham, M assachusetts June, 1973 PREPARED BY: I. APPROVED BY: ( Robert E. Bentley . :vY' ^ r 'M L v ;. . .....W j Aqu a t i c B io lo g is t : -/ .-iy-v;: Kenneth j . Mace.k, p h .D . :;V V i V :F;:i V v:i - vi'vi- i - i-VV ii ! y .. .. Company Sanitized. Does not contain TSC A CBI 'V j T a b le 1 ~ Acute t o x ic it y o f 8231 to rainbow tro u t3 (Salmo g a ir d n e r i). These data a re based on r e s u lt s o f b io a s s a y s conducted a t the a q u a tic :to x ic o lo g y la b o ra to ry of Bionom ics, In c ., Wareham, M a ssa ch u se tts. Compound 8231 \j-CPO-3 t :J(- 0 - m i l l i g r a m / l i t e r W hour ' , 96 hour ;2 9 2 -5 0 6 )b >100 <140 No E f f e c t Level (m g/i) ^9 < A ssa y conducted a t l i e ( - 1 . 0 ) , mean w e ig h t of rainb o w tr o u t 1 .3 g . Si 1 b95% confidence in t e r v a ls . Asm Company Sanitized. Does not contain TSCA CBi