Document ymb06M9ZL8nqnR1M2VyEp4z56

AR226-2840 EIOASSAY REPORT SUBMITTED TO E. I. DUPONT DE NEMOURS & COMPANY '^gy A cute t o x i c i t y o f 8233 to rainbow Bionom ics, In c , 790 Main S tr e e t Wareham, M a ssa ch u se tts Ju n e , 1973 m S?ss ; / i EEssn*contain TSCACBI | ! METHODS AND MATERIALS This investigation was performed at the aquatic toxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of rainbow* trout (Salmo, gajrdneri) to 8233 (a light brown liquid tesjted as 100?S active) was measured in i- ' terms of the median tolerjance limit (TL^q ), the nominal concentration of the chemical in water which causes 50 percent response (death) under tha test conditions during a specified time interval. The prediction of a T L ^ Value, and its 95# confidence interval, was based on conversion of the concentrations tested and the corresponding observed percent mortalities to logs and probits respectively, and the subsequent mathematical calculation of a linear '' q t i a . The toxicity of the chemical tested wajs evaluated under static bioassay conditions for a 96-hour period. I The test procedures for the static bioassays are in complete accordance with the Pish ^ioassay procedure in the 1970 edition of Standard Methods (APHA) except for those conditions described below. CBICompany Sanitized. Does not contain TSCA Page two The rainbow trout used jin these tests were obtained from a commercial fish hatchery in Massachusetts and had a mean weight of 1 .3 g and a mpan length of 58 mm. The test population was held in the laboratpry hatchery facilities for at least 30 days prior to testing and during this period mortality was <2%. static bioassays Vere conducted in 5-gallon glass vessels held in constant temperature water baths at 11c (- 1.0). The vessels were not aerated, and there was a single introduction of the test compound into the test, vessels. A solvent control, utilizing the greatest amount of isopropyl alcohol contained in any test vessel, was'conductid. fish were acclimated to the test system containing reconstituted Water as the standard diluent for at least 2h hours prior to testing. Ten fish were exposed in each test vessel. reconstituted water used in the test system was jweparedj:% adding the following chemicals to deionised viraten Har dness 38 mg/l mg of salt/llter wa ter_________ Na^COj ca.SOjj; - MgSO^ KCT 48 30 30 2 The pH of the standard diluent was 7.1. and the methyl iI . orange alkalinity was 3fj ppm as CaCQ^.. Dissolved oxygen values for the various iest vessels ranged from 8.5 initially to 5.3 mg/l atj the end of the tests. TSCACBt not contain SX* 5S a n * * . Com paq The predicted TL^Q values;(95% confidence interval) are j presented in Table 1. Tatjle 2 presents a summary of observed i ' . mortality for the bioassayf after and 96 hours of exposure. Moribund fish generally beicame dark and lethaigic, lost equilibrium, and expired. i m Vy ''f $>>. -: r. - ; 1 Company Sanitiz,ed. Does nof contain TSCACBll SUBMITTED BY: Bionomics, Inc. 790 Main Street Wareham, Massachusetts June, 1973 PREPARED BY: Robert E. Bentley APPROVED BY : Kenneth J. Macek, ph.D. Director c Ofn: Ooe *fiot 'in *S c A C8t Table 1 -- Acute toxicijty of 8233 to rainbow trout (Salmo gairdneri). These data ape based on results of bioassays conducted at the aquatic jtoxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. Compound 8233 - milligran^/liter iour ! 9b hour 8.63(6.94-10.90)b 5.64(4.14-7.69) No Effect Level (mg/l) 4.20 Assay conducted at 11c (-1.0), mean weight of rainbow trout 1.3 g. 395% confidence intervaljs. Company Sanitized. Does not contain TSCA CBi ! 1r Table 2 -- Concentrations of 8233 tested and corresponding observed percent mortality for rainbow trout (Salmo gairdneri) at 24 and 96 Hours during a static bioassay. Compound 6233 Conce ntration (mg/1 ) 18.0 j 14.0 i 10. 1 . . 7.5 5.6 4.2 3.2 contr Ol ,, cntr ol (isopropyl alcohol) ... 4' hour 96 hour 100 100 100 100 4o 100 50 100 0 20 00 00 00 00 Company Sanitized. Does not contain T S C A CBI