4 I il PFOS: A 48-Hour StaticAcute ToxicityTeSt with the Cladoceran (Daphnia magna) ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.

-- .. - -- .-.-.-.-.-.-.-..- .- .-.-.-..-....- - - hi,-,lhevels)fparticulatmeatter.The Environmenta IQualityBoard of Puerto izicohas statedthatCata fio(an industrializeadrea) has a historyof exceeding @atit)naAlmbient Air QualityStandards.The Hea lthDepartment of Puerto Rict)has reportedthe highestratesof respiratoriyllnessesand alltypesof ::inccrisn thismunicipalityU.sing theEPA standardsampling methodology, 1,)tasluspended particles(TSP) and particulatmeatter of lessthan 10 @Lm @P\110)were collectedinCatafioand Fajardo(controlarea)foreach season of the year.The particulatmeatterfrom the air filterasnd from reference ,tandardmaterial(SRM 1649. urban dust)Were extractedsequentiallywith threseolventosfdifferentpolaritieussing 24-hour SoxhietextractionT.he extractsobtained were testedfor cytotoxicitoyn human keratinocytesusing the NeutralRed Bioassay (NRB).

FC-17-R Submitted to: BIOSEARCH, INC.

3M EnvironmentaLlaboratory FinalReport-AnalyticSatludy Single-DoIsnetravenouPsharmacokinetSitcudyofT-6054inRabbits In-VivSotudyReferencNeumber:HWI#6329-138 StudyNumber: ANMT-122094.2 TestSubstanceF:C-129(T-6054) Name and AddressofSponsor: 3M SCD Division 367 Grove Street St.Paul,MN 55106 Name and Address ofTestingFacility: 3M EnvironmentalTechnology& Services 935 Bush Avenue St.Paul,NlN 55106 Method Numbers AMDT-M-1-0, AMDT-M-2-0, AMDT-M4-0, AMDT-M-5-0, AMDT-M-8-0, and Revisions: Thermal ExtractionofFluorideby Means of a Modified Dohrinann DX2000 OrganicHalideAnalyzer-Liver FluorideMeasurement by Means ofan Orion EA940 Expandable Ion Analyzer ExtractionofFluorochemicalsfrom RabbitLiver Analysisof RabbitLiverExtractforFluorochemicalsUsing ElectrospraMyass Spectrometry Analysisof FluorideUsing the SkalarSegmented Flow Analyzer with Ion SelectivEelectrode InitiatioDnate: See attachedprotocol Author:James D.

,)'HAZLrm%CN LABCNDMMRIEB AA/ERICA, INC. 3301KINSMAN BLVD. p.o.BOX 45 MADISON, WISCONSIN 53707 Chemical& BioMedicalSciencesDivision PHONE (608)241-4471 TLX 703966HAZRAL MDS UD FINAL REPORT JUL, JANINE GLEASON MINNESOTA MINING & MANUFACTURING TOXICOLOGY SERVICES ST.

Experiment No.: Conducted At: Dates Conducted: Conducted By: Acute Oral Toxicity Screen with T-2574CoC in Albino Rats RECEIVED OCT - @ 1979 31 TOXICOLOGY 0979AR0037 Safety Evaluation Laboratory Riker Laboratoriest Inc-t St. paul, minnesota August 6, 1979 to September 6, 1979 i?

-@ l@@ 1@4 -I----m cov ArgoooCoomE@m THE DEVELOPMENT SERVICES COMPANY DraftReport 13-Week DietaryToxicityStudy withN-Methyl PerfluorooctanesulfonamiEdtohanol(N-MeFOSE, T-6314) inRats PREPARED FOR: 3M St.Paul,Minnesota COVANCE STUDY NUMBER: 6329-225 COVAW-C'E'."'

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE .IdentityP:erfluorooctanesulfonylamido(ethyl)acemtaaytea;lsobe referredtoas PFOSAA, FC-1 28 or as the major component of FC-127, FC-1 29, FC-109, or FC-1 09-X.

BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95 or as a component ofFC-201 AF. (1Octanesulfoniaccid)(CAS # 2795-39-3).

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (COROPHIUM VOLUTATOR) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonicacid)(CAS # 2795-39-3).

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component of FC-203CE. (1Octanesulfonicadid)(CAS # 2795-39-3).

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonaiccid)(CAS # 2795-39-3).

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfbnatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-203S. (1Octanesulfoniaccid)(CAS # 2795-39-3).

ACUTE TOXICITY TO FISH (RAINBOW TROUT) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3).