Document vVxq95bkZX96vmyEd5ayev02q
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesuftnatew,hich may alsobe referredtoas PFOS, FC-95, oras a component ofFX-3104. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was notnoted.The testsample isFX-3104, 6%. Currentinformatioinndicatesitisa mixtureof0.48% PFOS, 14.09% diethyleneglycolbutylether,59% water,17.6% ethyleneglycol, 1.44% sodium octylsulfate4,% inhibito2r.,0% polyoxyethylene monooctylphenylether,1.25% propane sultonefoanier,0.03% sodium lauryl sulfatea,nd 0.1% benzotdazole.
The following summary applies to a mixture with incompletely charactedzect concentrations of impurities.Data may not accurately reflectthe toxicityof the tluorochemical component of the testsample.
METHOD:
Method: Not given. Type: Acute static GLP: No Year study performed: 1985 Species: Selenastrumcapricomutum Source: Not given. Element basis: AJgalcellcounts(cells/mL). Exposure period:96 hours StatisticaMlethods: lCw valuescalculateudsingprobiatnalysisorother regressionmodels. Analyticalmonitoring: Not given.
Test Conditions:
AlgalNutrientMedium: Not given. Stock and testsolutionprepamtion: Not given. Exposure vessels: Not given. Agitation:Not given. Number of replicates:2 Initiaclellloading:2.05x 104 cells/mL Number of concentrations:fiveplusa blankcontrol Lighting:Not given. Water Chemistry: Not given.
RESULTS
Nominal concentrations: Blank control,100, 180,320, 560, and 1000 mg/L
AlgalGrowthResponse
NominalConc.
(mg/L)
Control
100
180
320
1
560
1
1
1000
1
Mean Coll-Count (colls/mL) 1,790,000 1,730,000 1,620,000 1,160,000 980,000 210,000
Element values: 96-hour IC50= 576 mg/L
PercentInhibition
--
3
9 35
45
88
7:1
Element valuesare based on nominal concentrations.
Reversibilityof Growth Inhibition:Not given.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance exhibitsa 96-hourIC5oof576 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking3. Study lacksdescriptionand recordsofthe method followed.There isno characterizabonofthetestsubstance purity, and the studylacksconfirmationoftheamount offluorochemicalproportionin the solution.
REFERENCES
Thisstudywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Cl 111-2,1985.
OTHER
Last changed: 6/26/00.
ENVIRONMENTAL LABORATORY
LR
FRESHWATER ALGAE GROWTH INHIBITION TEST
ALGAL CELL COUNT, no./mi
HEMOCYTOMETER METHOD*
TEST* DAY.
TEST TYPE
DATE
MATERIAL TESTED:
1,5@cl-Ir
ALGAL SPECIES'! Qreen Algae. Selenastrum capricarnutum
ANI@LYST TREATMENT
TOTAL OF 25 FIELDS COUNTED
BC lis
no./ml
MEAN + SD
eCO IOC)(D
I1-15clio0c)
-37,C 1-3
2--17D
-4
(cc)
i!sIt-j
Ioc"
Z-0
Lo;?,IoCoo
I ISO,()C)o I I@0c) coo
QUO)OCO.
I OCC)10C)o
ac) @'iot C)O 0
0.1 mm deep Hemocytometer; area of field 1 x 1 and 0.1 mm deep.
ci
ENVIRONMENTAL
LABORATORY
TABLE 1. PUMMA
OF ACUTE TOXICITY
0F ItX- 4U I:y 4 _VCP
TO FRESHIQATER
ALGAE
(SELENASTRUM
CAPRICORNUTUM)
Chemical Concentration (mg/1)
Control
Percent Inhibition in Standing Crop (2)
48-Hr. Based on Cell-Count
Based on Cell-Count
96-Hr. Based on
Cell Dry Wt.
E38
ic (95% Confidence
50
Limits) mg/l (3)
Acceptability of Test
The Selenastrum capricornutum following quality criteria:
control cultures have met the
- Exhibited log phase growth within _qjp@7 hours (max. 48 hr.)
-
Attained a 96-hr. standing
crop of l,t7qxl cells/ml
5 (min. 10
cells/ml)
Loci "Dkase @qmwNR%,-&4 4?)- @x@. 4-4,S)OC)o
(1) Data were averages concentration.
of two replications
for each test
(2) Based on cell-count (no./mi) and/or cell-dry weight (mg/1).
(3) IC50 values were calculated using Probit Analysis regression models of experimental data.
or other