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Microbics saltof PFO
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MICROBICS MICROTOX@ TOXICITY TEST
TEST SUBSTANCE
Identity:PerfluorooctanesulfonaLtiet,hiumsaltm;ay alsobe referredto as PFOS LisaltF,C-94,orFC-94-X. (I-Octanesulfoniacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadeclaiftlhuiourmsoa-l,t, CAS # 29457-72-5)
Remarks: The testsample isa mixtureofthetestsubstanceinwater (approximatel2y4.5% testsubstanceand 75.5% water).No calculations were made to adjustfortheactualconcentratioonfthetestsubstancein the testsample.
METHOD
Method: MicrobicsMicrotox@"BASIC" Procedure Test type: Staticacute GLP: No Year Completed: 1994 Species:Photobacteriupmhosphoreum Analyticalmonitoring:pH and osmoticcondition Statisticmaelthods: EC1 0 and EC50 valuescalculatebdy statistical linearegressionprogram providedforMicrotox@) Test organism source: Microbics,Reagent Lot# AM025. Test conditions
Dilutionwater: MilliporMeilli-Q"wmater Stock and testsolutionspreparation:A primarystocksolution was preparedindilutiownaterat2000 mg/L. The pH ofthe stock solutionwas then adjustedfrom 5.9to7.0using0.02N NAOH and an osmoticadjustmentwas made using200 mg NaCl dissolvedin 10 mL of stocksolutionA.ppearance oftestsolutionwsas noted as "clearand colorless"A.lltestsolutionwsere made by proportional dilution. Exposure vessels: 3 mL glasscuveftes. Number of replicates:2 Number of concentrations:Four concentrationasnd controls Element basis:Percentlightloss
RESULTS
Nominal concentrations:125,250,500, 1000 mg/L Element value: 15-minuteECIO = 564 (367-866)mg/L
15-minuteEC5o = >1 000 mg/L 30-minuteEC,o = 487 (320-738)mg/L 30-minuteEC5o = >1000 mg/L
Remarks: Values reportedareforthetestsample. No calculations were made to adjustfortheconcentratioonfthetestsubstanceinthetest sample.
CONCLUSIONS
The testsample containing24.5% PerfluorooctanesulfonaLtiet,hiumsalt exhibiteda 30-minuteEC50 forPhotobacteriumphosphoreum of >1000 mg/L.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St..PaulM,innesota,55133
I'@, DATA QUALITY
ReliabilityK:limischranking2. Thisstudy,whilewellconducted,lacks analyticadlata for:determinatioonfthe testsubstanceconcentratioinnthe testsolutionsa,nd determinatioonfthesample purity.
REFERENCES
Thisstudywas conductedby the3M Company, Environmental Laboratory,Lab Request number M 1018,2/07194.
OTHER
Last changed: 512/00
ENVIRONMENTAL LABORATORY MICRO131CS MICROTOXO TOXK:ITY TEST
SAMPLE: FC-94-X
LR M1018 JBD 2t7/94
Test Conc.
(mg/L)
125 250 500 1000 EC50, mg/L (95% C.I.)
PERCENT LIGHT LOSS OF P. phosporsum AFTER:
5-Minf,fa=&ule
15-MinE.j=sure
0 0 0 2 > 1000
7 5 9 15 > 1000
8 7 10 18 > 1000
ample Desc.-iption LA
Page
ot
Environmental Lab - Work Sheet
Oate:
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Analysol:
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:vl-TCROTOX DATA REF:@OR!
FILE NAtoIE:MlolS.K30
TEST DAT;:'.
Li
TEST TilmE..
Inves-i--i-aator: Samc'@e Description:
M4@crotox Testina
Approv,:---dov: o-F
Procedt-,,re-.BASIC
Osmo@'-ic Adjustmen"-: NaC!
Initiaj' Concentratior,
ma/L
Test Time-. 30. mit-iutee
Fi. -----
I TT ------------
DilLtt'LonFactor : 2
Un,-;ts.-ma/:
C 0 NL-,* ----------
GAMMA ---------
.i
97 .94/' /r7.IJ5
2
97.7-7! 75.86
125.0000 5 .00
O@07276 O.Oq498
CONTROL
'o-9.54/ -/3, ie-
2 5 0. t)
4
7
5
96 .4/
4
5()C, C,
qC,.-57/ 71). 2.'_:'
5 0000
7
93.75/ 64.43
1")Ot@j,.()Ooo
.3
9"4.2t*e' 64.48
1000.000i@
IT@'IO =
q6.69 63.78/ 97.62-p*
0. 0-'!140L7#
1
244
0.11509!@
0. c)9495-4
(D.'-73597#
0.24)221#
RATIOS =
@34()6
0.2532w*
CORRECTION FACTOR = ).C-494
10 ZD DDDDDDDDODDDDODDDODDDDI--, .3SI-OPE O.C-6(--@7 3
C, 3 A m
A
ODDDDDDDDDDDDD
DDRM-DDDDODODDD DD ODD D?
.3 .3 3 3
1 >zzzzzzzzzzzzzzz--:,zzz-7zzzzzzzz-.
.3
.3 .3 .3
..10 ODD 100
a
4
6
DDDDDDDDDDTDDVDDD-DDDDVD
1000
E5
DDD. DDT)DDDDDD-DDD CONCENTRATIQI* lOE4
3
'7
3 .3
-T)D,-,!)DDDDL.-)-V,D7DDDDY I C)E
(95% CON*R-IDENCE RANGE: 600.'7Sa6 7r--'I'l-5462.l@29-@i)
STATIS71CAL REPORT FOR DATA FILE Mlt)18.e*--Ii)
ESTIMATING EQUATID.N.- :-OC-0 r@.8:351 x LOrz b NUMBER OF DATA PAIRS US=D.- 6
RE=TD;_iA'-
ITANCE: 0.0254C-
95% CONr-IDENC;-r r-ACt--"-iR-5..07*----,2-FlOyR EC5o
CC.RRE'@-AT--C!N @:'-)Ei--;z'LCIENT@,:R
U-@IEFFTCT@;4T OF DETERMTNATION:
R"'@2 O@74379
EFFECTIVE CONCENT7<r,--i!ONS (95% 'L-ONFT-'-."E,,RiACNEGE'T.
EC50 EC20 ECIO
=-)048.3767 = ?57.7f-96 = 466.5695 = 65.6808
ina/L ma/L mall mci/L
(600.9,231"Z Tlr_'"jf'..'L5462.1i.E3*q5l**4 (474.9458 TO l@931.4671-I-) (3c@-0.5834 TO 738.4969)
1(:).7405'TC, 4C,1.6@@49)***
E X.-.i-"--@APOLATEA---'-.;-7.SUT'S
INIT'.RAL DATA
I\iljlv',BER
1 X3 4 5 6
CONt@,@CNTRATION
4L 2 -=i'tr.0-)o 0 .4L25.0000 25(.).01)0 0 25'--'. 500.0000 500-0000 io0i).0000 10()0.0()Ot@
0 i@3 )95
0.04t) o0.. 11@-,:?
il=i -d.1)95 (@j.27@6
IS K3 0
EFi=l-=CT
6.7PS.67 3.6-2 9.85 10.32 8 .ez'7 19. .@)9 16.62
7.7::, 6.86 9.5.,-) 'L7.96
ii-.cf-L-i-CTouch SPAC-M BAR
10
'IICRL '@@X DATA REPORT
FILE NAME: NIOIS.K!5
TEC,[-'L-ATE.-
TEST TIME:
Aporaved by;
-zamole Descrioti oFi: Microtox Testina of FC-3l't-X
Procedure: BASIC -
Adjustmen"--: NaC!-
iriit4-al Concentration : IOC%.,r"r,a/L
Dilution Factor 2
Test Timel- 1&5 minutes
ancentration Uii-ts.-ma-I.L
NUMBER
i(.)/IT -------------
cor4c. ----------
13AMMA ---------
@l
97.34/' ?'6.80
2
97.79/ 7t.09
96..@il-77*4@l'
3C'2.54/ 7--,,-:15
6
-iO.53/ 69.03
7
93.75/ 65.86
c-
9'L . @C-6/ 6Z-:. J.4
i 0000 12 C,, 0 0 2 5 0. 0
0 . i)0 0 0 .5()C). ioo().O()00 1ooo., 0.,),.)o
0.06503 (,*@ C,@E-76i o.o2al7#
.97 2 2 ().09912# o.09526# 0.188814
17()432#
Ot'--NI'ROL1-1/10 = 80.4-6/ 96.69
97.62**
RATJ@OS =
i).-.I-F--.r71-
CORRECTION
FACTOR = O.A-351
iO ZDDDVDDDDDDDDDDDDDDDDDVDDVDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD?
3SLORE
.3
1:1 .3 r_. .3
3 J. -,Z.,-r-@ZZZ-T@-"-@-@zzzzzzzzzzzzzz_lzzz-.
3
:EC5,)
.3
4
6
.lo ODDDDDDDDDDDDDDDDDDD@DDDDDDDDD.DDDVDDDDD"-DDODDDDDDDZ@IDDDDDDDDY
iCIC,
1000 CONCENTRATION
I'--,E4
.3 .3 .3 .3
1t)E5
E 05o7(";P6.
-?5*1'.CON7*-'DENC:--- @@jiANG-E.-
TI-@j%l@@-917.8008)
Used
calculat4@cr-a
ETA-irIET'@LCAL REPORT FOR DA-grA FILE M101E.K!5
ESTIMATING
EQUA'7!CN:
LOG C = 0.7754 x LOG b +3.4?05
NI-'PMBER OF DATA PA@rRs U-OEL,f: 6
RESIDUAL VARA'.ANCE.-
?5% 'L-,L-JNFI!-WC@F-ANCCTEOR:
5.1401a
FOR EC50
C't@a'-%'RELATION R-'CEFF ILC TLENT: R
0.047@0
COEFFICIENT OF DETERMINATION:
0.7*@Lti76
EFFECTIVE CONCEN-IRAYIONS
(95% CONFIDENCE Rf-@NGEI:
EC@'O =3096.7422 EC20 =1056.9373 ECIO = 563.5806 ECO-1 = S7.7866
MO/L mall ma/L ma/L
( 602.4564 TO ( 488.4282 TO
-:-6'0.85e@-T2O 865.8c.-167) 13.3214 -17'042c).6264)*4@*
-cXTRAPOLA'@-ED @,'E:--"t-"LTS
I-N-@TIAL DATA
2 3 4 5 6
7
CONCENTRATION
125.0(:)00 125.000o 250.0000 250.0000 500.0000 '1020a@.C)0 (-@.)1,
.1000.0000
GAM.viA
0 @,)65 O.OGP-
1).087 0.0917 0.095 O.J.69 o.'L7C,
mioi C-. 5 EFF=-CT
@.74 8.02 13.@@-)4 S. 7,")
7
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MIS@-ROTr-'X DA'I'*AREPORT
FILE NAME: M1018.K5
TEST DA7E:
T@nvestioator:
TEST TIME: Aooroved bv.-
S,::tmoleDescrioticn: Microt@ax Testine of FC-?4--A
Procedure: BASIC
initial Concentration : 1000 ma/L Test Tirrie-.5 minut*es
NUMBER -----
J@O/IT ------------
Osma'iz Adjustment;
DilLt4,ionFactor : 2
Co-icentra4-.;-onUnits:
c 1-1c1!N ----------
GA!Oti!A ---------
i
?7.94/ 85.74
12
97.79/ 83.68
3
Sq-54/ 80.
4
7-
82.60
5
8 4,.114e
9().Z::.8/0.46
7
q3.75/ 77.91.
A
91.26/ 7$3. 10
-'.GN7ROL IT/IO = 6'-7,,959/6.69
RA-'Jv:D-0=
).8579
CORRE%@ITION FACTOR = 0.8587
4--25.0000 25. Ot)O,)
)00t-',, 0000 50t).()ot.,C) io0o.ilao0o1000.0ooo 63.ql@/ (?-f.62-"* o.Bff96**
-0.01-90--D* M 00352*
--0,04506* 0.00667*
-0.017674 -O.4'j3404*
0 -0.@1332 0 .0 0 3
NaC! ma/L cx
4fneaLs-
..........N.O TOXICT LTY Af U-ONCENTRATIONSTESTED Invali@-: r--'ataor controis
...14-ITIALDA'TA N U M F,
CONCENTRATION
125.004)()
2
125.0000
2 5 0 -X)
-z50.OOOO
C@
500.0000
6
500.0000
7
l@000.000()
1000.0000
7 ZNVALTLD DA-ra PEMOVED
,III Ifl1-1mI!i-ii-I (BA@IMA
-O.o!9 0. @",04 045 0.007.'*
-0.034 -
0.033
0.003
FOR C A L C U
0 @N'
@l4L@)1,3.K5 !il."IIM"IliiiIif-iIIili7
EPFER-T
-'t.72 0.66
-1.82 -3.52
3.22 r;.34
-0. at)
-2.67 1.76
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