AR226-3339 --------------------^^ Roy S Thompson (AstraZeaeca, Brixham, UK) Haria Colombo Watze de Wolf (Seh'ay Sotexis, BaHate, Stely) (OuPoBt, Mechelt'n, Belgiaaa) David G Fanrar Robert A Hoke Jacques L'Haridon (laeos C&ior; Ruacorn, UK) (DaPont, Newarfe, BE, USA) (CST, Evreux, Fraace) for.

- 9 Ape 22,3003 Ch0riMs 1mShaaman Ar22-1692 StfH EoPssHenBduigdainnegrs r0 mpaonm DmE Seioen, Dese Ms Whitman, As today ionmota EaaieDyy,o wseiehmssassopaeclieadlmaapptriooprninmeefogrmeh0e sreius e{ommorniumea Peerfluorioeohcstsapnsoaotmety(Cse8en)cropanrndombeelneetmtapl1erbpoestursa(ttehdeorntthee uensuespedcti$n1g5p0ub1l)ic by 1 worked foratshoe DuncPoonvtoeCmoemnpgaemnryvoufocsrhotmsaennoy yesnarestaonaodayhoaruvesandoiirmeyocmtoklunsaolnwylSebdiegceaeouesfeot|hseeirhre for my safety as a result ofwhl this company might doo meifmy identity were known `While I worked olan 0 management snd wh regrly etend by thir for them, like some other Dupont workers, I regularly tried to do the right corporante "isseecuroibtayco"kfigtwoohoenonpsutufbhoeirydcdopoeniugangbtaslno.ys[ohdunniydhonmfoyptwohiwinhneaognruwttrdotsonopigDe.nunpdTooihnnnetlayrnheydelemmyoynaencyda.4nedfaTrhtkhedaeitfatehreeynt O altruistihcn eDnfuplooonotkohissapvaswrhotraoretfpetshtahcoyeendpCpacsotor.rpiiRorannabtie1Bdg0tidnc.oym1pyetaevnnOrdcyeh,iownnHredoesintfygf.eorTenhdcieyassnDdauponovnertatndNo Ee Their actions are et de. corr and the direct result of informed aonhdedrealiibsercarteeudemcuisnitonhsebyegDaulPpornotces 5 `pertains to the use of and the environmental release and/or disposal of C8.

CENTRE FOR XENOBIOTIC RESEARCH UniversityofDundee, BiomedicalResearch Centre,Level5,NinewellsHospitaland Medical School,Dundee, DD1 9SY Study Report Determinationof cyanideinsensitivPeaimitoyi-CoA oxidationinsamples from 3M Environmental Laboratory - Covance Studies6329-183 and 6329-212 Study Number XR0108 Author: Approved: t.M.Elcom,be C.R.EL .e- XR0108 PaLyeI of 3 Date: I Introduction The purposeofthisstudywas toinvestigattehelevelsof PCOA oxidationinthefrozen liversamplesprovidedby 3M EnvironmentalLaboratoryfrom Covance studie6s329-183 and 6329-212. 2 TestSubstance 6329-183:PFOS, 20ppm, administeredby Covance untilweek 4. 6329-212:ETFOSE-OK 100 and 300ppm, administerebdy Covance untilweek 4. 3 TestingFacility CentreforXenobioticResearch,UniversityofDundee, BiomedicalResearchCentre, Level5,NinewelisHospitaland MedicalSchool,Dundee,DDI 9SY, UK. 4 Homogenste Preparation 4.1 Reagents SET buffer0:.25M Sucrose,5mM EDTA and 20mM Tris-HCI,pH 7.4 4.2 Instruments Potter-Elvehjehmomogeniser 4.3 Method Frozen liversampleswere thawed on ice,blottedand weighed.A 25% homogenate was preparedinicecoldSET usinga Potter-Elvehjehmomogeniser(6passes). 5 Determinationof Cyanide-in.sensitPiavlemitoyi-CoOxidation The 25% homogenate liversampleswere dilutedI partin 10 partswithicecoldSET.

PerfluorosulfonamiMdeetabolisminRatvs..Hum-an--Heya R C H CH S 0 -2N -%.

-4 HAZLOTON L"BDPffDRIES ANFERICA INC Chemica&l BioMedicaSlcienceDsivision @'N@iol orK.I-NSsMoAN BLVD. - P.O.BOX 7545 * MADISON, WISCONSIN 63707 PHONE (6082)41-4471* TLX 703956HAZRAL MDS UD FINAL REPORT ROGER PERKINS MINNESOTA MINING & TOXICOLOGY ST.

LaboratoryReport AnalyticalReport ofData for PFOSA PharmacokineticStudy inRats (Seraand Liver) Laboratory Report No.

p. 7 CAIN ~ E a 0~ ~ ^1 C/) O U C: 0 CL L.

.

3 .

AR 226 -- 14S 1 TE En wo Ro A Darby rsa ob "a?

.

B pp Ese ' ici M6 AR226 - 0627 | | 3M Fluorochemical Initiative Environmental Program .

Northwest Bioanalytical Study No.

1 RESEARCH Precise Research.

232.1 441572.0,4346794.0 441577.11.4346769.53 441582.21,4346745.05 441587.32,4346720.58 441592.42,4346696.11 441597.53,4346671.63 441601.0,4346655.0 441579.88,4346641.63 441558.75,4346628.26 441537.63,4346614.89 441522.0,4346605.0 441503.0,4346601.0 441483.86,4346584.91 441464.73,4346568.83 441445.59,4346552-74 441434.0,4346543.0 441448.9,4346522.92 441457.0,4346512.0 441444.34,4346490.44 441431.68,4346468.89 441420.0,4346449.0 441401.14,4346432.59 441382.28,4346416.18 441363.42,4346399.77 441344.56,4346383.36 441325.69,4346366.95 441320.0,4346362.0 441324.51,4346337.41 441329.02,4346312.82 441333.54,4346288.23 441338.05,4346263.64 441340.0,4346253.0 441364.75,4346256.51 441389.51,4346260.01 441414.26,4346263.52 441439.01,4346267.02 441463.77,4346270.53 441488.52,4346274.03 441513.27,4346277.54 441538.02,4346281.04 441562.78,4346284.55 441587.53,4346288.05 441612.28,4346291.56 441637.04,4346295.06 441661.79,4346298.57 441686.54,4346302.07 441711.3,4346305.58 441736.05,4346309.08 441760.8,4346312.59 441785.55,4346316.09 441810.31,4346319.6 441835.06,4346323.1 441859.81,4346326.61 441884.57,4346330.12 441909.32,4346333.62 AR226-2368 EID419917 441912.0,4346334.0 441908.73,4346358.78 441905.45,4346383.57 441905.0,4346387.0 441929.58,4346391.54 441954.17,4346396.09 441978.75,4346400.63 442003.33,4346405.18 442027.92,4346409.72 442052.5,4346414.27 442077.08,4346418.81 442101.67,4346423.36 442126.25,4346427.9 442143.0,4346431.0 442146.0,4346416.0 442170.16,4346422.44 442176.0,4346424.0 442180.0,4346402.0 442204.18,4346408.36 442228.35,4346414.72 442252.53,4346421.09 442275.0,4346427.0 442272.99,4346451.92 442270.97,4346476.84 442268.96,4346501.76 442267.0,4346526.0 442291.43,4346531.29 442315.87,4346536.58 442340.3,4346541.87 442364.74,4346547.16 442389.17,4346552.44 442413.61,4346557.73 442438.04,4346563.02 442462.47,4346568.31 442486.91,4346573.6 442498.0,4346576.0 442493.0,4346589.0 442517.57,4346593.6 442542.14,4346598.21 442566.72,4346602.81 442591.29,4346607.42 442615.86,4346612.02 442640.43,4346616.63 442665.01.4346621.23 442689.58,4346625.84 442714.15,4346630.44 442738.72,4346635.05 442763.3,4346639.65 442787.87,4346644.25 442812.44,4346648.86 442837.01,4346653.46 442861.58,4346658.07 442886.16,4346662.67 442910.73,4346667.28 442935.3,4346671.88 442959.87,4346676.49 EID419918 442984.45,4346681.09 443009.02,4346685.7 443033.59,4346690.3 443048.0,4346693.0 443043.47,4346717.59 443041.0,4346731.0 443065.57,4346735.61 443090.14,4346740.22 443114.71,4346744.84 443139.28,4346749.45 443163.85,4346754.06 443188.43,4346758.67 443213.0,4346763.28 443237.57,4346767.89 443262.14,4346772.51 443286.71,4346777.12 443311.28,4346781.73 443335.85,4346786.34 443360.42,4346790.95 443366.0,4346792.0 443361.52,4346816.59 443357.04,4346841.19 443352.55,4346865.78 443348.07,4346890.38 443343.59,4346914.97 443339.11,4346939.57 443334.62,4346964.16 443330.14,4346988.76 443327.0,4347006.0 443302.48,4347010.86 443277.96,4347015.73 443253.43,4347020.59 443228.91,4347025.46 443206.0,4347030.0 443181.38,4347034.34 443156.76,4347038.69 443155.0,4347039.0 443130.04,4347037.64 443105.07,4347036.28 443080.11,4347034.92 443055.15,4347033.57 443030.18,4347032.21 443008.0,4347031.0 442996.0,4347028.0 442971.0,4347028.16 442946.0,4347028.31 442921.0,4347028.47 442896.0,4347028.63 442871.0,4347028.78 442846.0,4347028.94 442836.0,4347029.0 442825.0,4347030.0 442800.03,4347028.86 442775.05.4347027.73 442750.08,4347026.59 442737.0,4347026.0 EID419919 442716.0,4347032.0 442691.26,4347028.38 442666.53,4347024.77 442641.79.4347021.15 442617.05,4347017.54 442592.31,4347013.92 442586.0,4347013.0 442574.0,4347015.0 442560.0,4347012.0 442545.0,4347018.0 442520.1,4347015.79 442495.2,4347013.57 442470.29,4347011.36 442445.39,4347009.15 442420.49,4347006.93 442395.59,4347004.72 442370.69,4347002.51 442365.0,4347002.0 442357.0,4347005.0 442350.0,4346997.0 442329.0,4346996.0 442315.0,4346995.0 442302.0,4346990.0 442285.0,4346985.0 442260.02,4346985.96 442259.0,4346986.0 442252.0,4346983.0 442227.7,4346977.11 442219.0,4346975.0 442194.88,4346968.42 442186.0,4346966.0 442161.12,4346963.51 442156.0,4346963.0 442131.44,4346958.36 442106.87,4346953.71 442082.31,4346949.07 442057.74,4346944.42 442033.18,4346939.78 442008.61,4346935.14 441984.05,4346930.49 441959.48,4346925.85 441955.0,4346925.0 441930.39,4346920.61 441905.78,4346916.21 441881.17,4346911.82 441871.0,4346910.0 441860.0,4346910.0 441848.0,4346898.0 441832.0,4346895.0 441820.0,4346891.0 . 441804.0,4346886.0 441780.19,4346878.39 441756.37,4346870.79 441732.56,4346863.18 441732.0,4346863.0 441708.82,4346853.63 EID419920 441685.64,4346844.26 441685.0,4346844.0 441663.0,4346841.0 441640.0,4346841.0 441619.52,4346826.66 441610.0,4346820.0 441595.0,4346819.0 441585.0,4346808.0 441572.0,4346794.0 EID419921