3M Environmental Laboratory R126-0005 M ethod A nalysis of Potassium Perfluorooctanesulfonate or O ther Fluorochemicals in Serum Extracts U sing H PLC-Electrospray/M ass Spectrometry Method Number: ETS-8-5.1 Author: Lisa Clemen, Robert Wynne Approved By: 'L l Laboratory Manager Adoption Date: 03/01/99 Revision Date: <V / 2 C / Date Group Leader Technical Reviewer Date dh i / m Date 1.0 S c o p e a n d A p p l ic a t io n __________________________________________________________________ 1.1 Scope: This method describes the analysis of serum extracts for fluorochemical surfactants using HPLC-electrospray/mass spectrometry. 1.2 Applicable Compounds: Fluorochemical surfactants or other fluorinated compounds, or other ionizable compounds. 1.3 Matrices: Rabbit, rat, bovine, monkey, and human serum, or other fluids as designated in the validation report.

P A U L R.

/H U & -0 3I OCTANOUWATER PARTITION COEFFICIENT TEST SUBSTANCE Identity: N[r[e(2fhe-e(rNpret-admdteeotchaaysfllpuNeo-rrMfoluoeocFrtOoyolS)csEtuaAlfn,oenosyrulM]lmfoeenFtahOmySliadEmoA)i.

/1IUX-0M7 ACUTE TOXICITY TO AQUATIC PLANTS (ALGAE) TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-X or R1904.

jg ttjM U W ttl ARaU-O M G3 'C

BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, FC116, FC-126, FC-169, or FC-143.

}r f .T lP * l t r a , S^/ U -l PA-rJT^ l f3 A-ik/TS t- fW } d v ^ - r s . *.

MSISL6- 068i> IN VITRO MICROBIOLOGICAL MUTAGENICITY ASSAYS OF THREE 3M COMPANY COMPOUNDS Final Report April 1978 By: Vincent F.

AU9M.70*t ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE_______________________________________________ Identity: Mixture containing periluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-201F. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 1.

- 0 7 Q -!

AR &Z& - 0 7 8 / ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3).

AR A 2 .6 _ O ilf-n ACUTE TO XICITY TO AQUATIC INVERTEBRATES (COROPHIUM VOLUTATOR) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-602. (1Octanesulfonic acid) (CAS # 2795-39-3).

M z i G - I D S 0!

p r r: a A R 6 _ !

COLUM BUS, O HIO OFFICE TW ELFTH FLOOR 21 E A S T S TATE S T R E E T CO LUM BUS.