Document 3Nbv0ra91Zrrj2Nd8BD8yde6J

AR &Z& - 0 7 8 / ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 1. The test sample is FC206. Current information indicates it is a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Not given. Type: Acute static and continuous flow GLP: No Year completed: 1974 Species: Pimephales promelas Supplier: Not given. Analytical monitoring: Temperature, pH, and DO. Exposure period: 96-hours Statistical methods: LC50 value calculated using graphic interpolation. Test fish age: Juveniles Length and weight: Average length = 2 in. Average weight = 1.6 g (wet) Loading: Not given Pretreatment: None Test conditions: Dilution water: Carbon-filtered city water, St. Paul, MN Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: A 50% primary stock solution was prepared for the continuous flow study. The test material was added directly to the dilution water on a volume/volume basis for the static study. Diluter Flow rate: Not given. Concentrations dosing rate: Continuous for the flow study, once for the static study. Stability of the test chemical solutions: Not noted. Exposure vessels: Not given. Number of replicates: 1 001459 Number of fish per replicate: Not given. Number of concentrations: five plus a blank control Water chemistry during the static study: pH range (0-96 hours): 6.9 - 7.0 (control exposure) 7.1 - 7.2 (1250 mg/L exposure)* Temperature range (0-96 hours): 67 - 72 F Dissolved oxygen range (0-96 hours): 4.7 - 5.7 mg/L (control exposure) 3.9 - 5.7 mg/L (1250 mg/L exposure)* * 1250 mg/L (intermediate concentration) exposure values used because the highest three concentrations resulted in total mortality by 72 hours. Water chemistry during the continuous flow study: pH range (0-96 hours): 6.9 - 7.0 (control exposure) 7.0 - 7.1 (2500 mg/L exposure)* Temperature range (0-96 hours): 67 - 72 F Dissolved oxygen range (0-96 hours): 6.0 - 6.3 mg/L (control exposure) 4.5 - 6.0 mg/L (2500 mg/L exposure)* * 2500 mg/L (intermediate concentration) exposure values used because the highest two concentrations resulted in total mortality by 48 hours. RESULTS______________________________________________ Nominal concentrations: Blank control, 500, 1250, 2500, 3750, and 5000 mg/L Element values: 96-hour LC5o (static) = 1750 mg/L 96-hour LC50 (continuous flow) = 3000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS____________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be 1750 mg/L under static conditions and 3000 mg/L under continuous flow conditions. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 001460 P1AQUALITY. Reliability: Klimisch ranking 3. Testing lacks record of methodology used and documentation. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 2083,1974. OTHER____________________________________ Last changed: 6/27/00 001461 F^O R M 1 0 6 2 0 - P W O cc: LAB REQUEST BOOK - 42-5W File: Water and Wastewater REQUEST FOR LABORATORY WORK Lab Reciuest No- TO: ENVIRONMENTAL ENGINEERING LABORATORY - BUILDING 53-6N Requestor Lab Personnel Name: p g \ f i E a c d y \ .Request Date____ Project No. _______ Prob. No. < Code # . Date Rec'd. Sample:. Analyst: m t e 'V V SamDle Date: /i'TEja^zzffaDate Needed: Hold SamDle Fbr---------\ --&ays Est. Time: . \ ____ z re s t . . apre Description Total Hardness, mg/l as CaC0 3 Calcium Hardness, mg/l as CaC03 "P" Alkalinity, mg/l as CaC0 3 "M" Alkalinity, mg/l as CaC0 3 pH Sulfate, mg/l as SO4 Nitrate, mg/l as NO3 Chloride, mg/l as Cl Silica, mg/l as Si02 Fluoride, mg/l as F Ortho Phosphate, mg/l as P Total Phosphate, mg/l as P Total Chromium, mg/l as Cr Hex. Chromium, mg/l as Cr Total Cyanide, mg/l as CN Ferrocyanide, mg/l as CN Iron, mg/l as Fe Manganese, mg/l as Mn Surfactant, mg/l as LAS Phenol, mg/l BOD, mg/l COD, mg/l (Short, Long, Dilute) Total Suspended Solids, mg/l Volatile Suspended Solids, mg/l Turbidity, mg/l Silica Total Solids, mg/l Total Volatile Solids, mg/l Conductivity, mhos Total Bacteria, No./lOO ml Total Coliform, No./100 ml Fecal Coliform, No./100 ml Fecal Streptococcus, No./lOO ml Ammonia Nitrogen, mg/l as N Kjedldahl Nitrogen, mg/l as N Oil and Grease, mg/l . & /O /L S S 0 l\{ f/A h n u r ct K~0--:/$7-TSr - */o/ . Hrs. s tu ff0 ft. - r r 1 ,7 S o 'p m X# o.z%A/ Date Completed: Hrs. Spent: Jb C .fhu) V/ 11> ^ .O O PPM 0 0 1 4 6 2 303CartwnlMi White -- Environmental Engrg. Lab Canary -- Environmental Engrg. Lab Pink -- Environmental Engrg. Lab Goldenrod -- Requesters Copy * 740101 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET Type Test . C ,F I()td __________ M aterial te s te d L - 3 2 L ^ L o t # ! Test organism tftVWndi LLg. ^- Avg. Weight . Date Started ,Cj - \\ -~~1 ___________ Date Completed _________ ^ - V ^ Time Started _ ___ (? ; p M ________ city vMDilution Water atieV CarkflVL.ftM BEST COPY AVAILABLE 24 hrs 48 hrs 72 hrs 96 hrs 740101 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET Type Test *-\^Va\aT ! sfaYio. M aterial tested / -- Loj T est organism TftVVcrifl M 'v w v iw ? Avg. Weight \ - ^ XVxoV^C Date Started J^L- W - > \ Date Completed __ Time S ta rted f t ;?o p M D ilu tion Water r\\y makl qfVr cA ott f iM 1 BEST COPY AVAILABLE 24 hrs 48 hrs D.O % Control \0 6 7o 5 .7 \n o 72 hrs D.O D.O ';y. ' .V v if 52. \o o 5o 96 hrs Voo D.O Hi 5oopf>/1 1 .0 5 o . \oo \ 7 ; 6 _ \ o o .... 7/ 5 7 \o o 5 .0 \o o \o o M,5 lo o 7 / Ho Add V \ - / ~\Soc> . \oo 7/ 55 5b 0 2 .0 0 375o \o o 72 11 ___Q . _ . 3 - 5 5xx?o * 6o. 1ii ~ rt~ 5 5 .....0 ---- 7. 4 4 -~ 1) 1 \ii1 Average Temperature ....12*. ..............7<? 5 5 " Comments : . 0o 0o -- "i "3$ e -.e .J I'': .. r- - . -- -------------- ----- -------------- r--------------------- i . .4 . . . . . . '! ; - , O.Ob a/05 0 2 5 o 3 7 5 0.5 2 2.0 Afo >0 g'O 001464