R226 JH 426 511024a67O:raScGohvaogne eSTyovmicyRas --_ `TRADE SECRET Study Title 90H--D2a4y67O8r:alSGuabvcahgroenSitcuTdoyxiinciRtayts Volume 2 of 4 Laboratory Project ID: DuPont-6554 ARRR6 -- 3244 Dupont PN || TEST GUIDELINES: U.S.

., "CPhartohnoilcoFgeyePdeienrgRSetvuidyewinaRnadtPsawtihtohloPgFyOwAo,r"kiJnugneGr1o7u,p20R0e5v.i)ewoThfeMpaatmhmoalorgyy Grleavnidesw wfarosmaa joint projectofthe 3M Medical Department and DuPont Haskell Laboratory.

Environmental protection investigations initiated in the 1990s proved extreme environmental hazard o f perfluorosur factants based on perfluorooctane carboxylates and perfluorooctanesulfonates including "branched" or "linear fragments" --C 7F 15C O O _ and --C 8F 17S 0 3_ produced "electrochemically" or by "telomerization o f tetrafluoroethylene", respectively.1'2 These substances are extremely stable in the environment, unhydrolyzable, and non-biodegradable, but they are actively bioaccumulated and migrate in the food chains.3''1 Major manufacturers of long-chain fluorocarbons (such as 3M, DuPont, Daikin, etc.) have ceased or cease the production and sale of fluorocarbons, replacing them with alternative shortchain substances.

., DuPont Chemicals and Fluoroproducts, 4417 Lancaster Pike, CRP 702-2211B, Wilmington, DE 19880-0702 USA uSA.d it.com) 1CLF-Chem Consulting SPRL, 3 Clos du Chataignier, BE-1390 Grez-Doiceau, Belgium ( .@.

Donots contsain TSCA CBI i CONFIDENTIAL DPT 435/984104 IN VITRO MAMMALIAN CHROMOSOME ABERRATION TEST IN HUMAN LYMPHOCYTES Sponsor DuPont SpecialityChemicals, Jackson Laboratory, Chambers Works, Deepwater, NJ 08023, USA.

PFOA Levels by Water District/Source and Percent o( 3tudy Subjects Reporting Water Consumption from Each Source PFOA levelsa (ppb) Location Households Total populatiorrd (% total pop) Study sample (% study sample) 1 .7-4 .3 0 .4-3.9 0 .25-0.37 0.08-0.13 0.06-0 .1 0.06-0 .07 0 .165 1 .0-5 .0 1 .75-1 .87 0.05-8 .6 Little Hocking, Ohio Lubeck, WV Tuppers Plains, Ohio Belpre, Ohio Mason, WV Pomeroy, Ohio Blennerhassett Dupont, Washington WorksF GE Ptasticsc 68 Private Wells WVA & Ohio 4200 3700 4800 6000 4200 1000 71 N/A N/A 68 11,760 (17%) 10,360 (15%) 13,440 (19%) 16,800 (24%) 11,760 (17%) 2,800 (4%) 200 (0%) 2,200 (3%) 700 (1%) 190 (0%) 70,010 161(27%) 219(37%) 19 (3%) 45 (8%) 4 (0.7%) 1 (0.2%) 6 (1%) 90(15%) 43(7%) 11(2%) 599 'All PFOA Levels were reported by Qieir respective water dstricts and are available via the Internet . bEstimaled as 2.8 people per household.

Key words : PFOA, PFOS, uric acid This research was funded by the C8 Class Action Settlement Agreement (Circuit Court of Wood County, West Virginia) between DuPont and Plaintiffs, which resulted from releases into drinking water of the chemical perfluoro-octanoic acid (PFOA, or C8) .

Specific Objectives for TRP funds for Year 1 (from the research agreement appendix): Basedon the October26, 2001 meeting that included the Mabury (Toronto), M uir (NWRI) and Sibley (Guelph) andHoke, Buck, and Kaiser (Dupont) and Libre (Atojina) the specific work to be continuedand expandedfo r the TRPfunded portion o fthisproject is tofocus in year 1 on three specific areas and include the following: 1) analyticalmethodologies;2) environmentalmonitoringand 3) environmentalfatedetermination.

PFOA Levels by Water District/Source and Percent of Study Subjects Reporting Water Consumption from Each Source PFOA levels3 (PPb) Location Total population* Study sample Households (% total pop) (% study sample) 1.7-4.3 Little Hocking, Ohio 0.4 -3 .9 Lubeck, WV 0.25-0.37 Tuppers Plains, Ohio 0.08-0.13 Belpre, Ohio 0.06-0.1 Mason, WV 0.06-0.07 Pomeroy, Ohio 0.165 Blennerhassett 1 .0 -5 .0 Dupont.

& AR2222G6_ 1304 DonalKdP.reDsuindceannt iTrnedy 26 if March 14, 2003 "A"TTshhseeisUHtnoainntoterAdadbSmltieantiSesstterEpanhtveoinrroL.n Johnson mental Protection Agency, Headquarters O1f2f0i0cePoefnPnrseyvlevnatniioanA,vPeensutiecides and Toxic Substances WRaosohmin7gi1o0n1,MDC 20460 g3 22 3s 35 =- ZB5o3 2 28 b=d = Dear Mr.

Boutonnet English Translation of Report Summary for DuPont Chemical Solutions Enterprise 27-Jan-03 jppmpany Sanitized.

Key words: PFOA, PFOS, uric acid This research was funded by the C8 Class Action Settlement Agreement (Circuit Court of Wood County, West Virginia) between DuPont and Plaintiffs, which resulted from releases into drinking water of the chemical perfluoro-octanoic acid (PFOA, or C8).

RESEARCH DEFLUORINATION Low-temperature mineralization of perfluorocarboxylic acids Brittany Trangl t, Yuli Xiao-Song Xue4, Mohamed Ateial f, K.

. ^ nSH~, 3' CONFIDENTIAL DPT478/992105 MOUSE MICRONUCLEUS TEST Sponsor DuPont Specialty Chemicals, Jackson Laboratory, Chambers Works, Deepwater, NJ 08023, USA.

7 AR226-2028 ji*3g^^^i$g65#4i62.>*.v3vV^RS F C-8 SAMPLING PROJECT PAGE 1 OF 41 SUMMARY OF SPECIAL C-8 SAMPLING PROJECT Jennifer Nichols Environmental Lab LSE 9-16-97 ) zmzm\ BID093252 E ID 093252 C-8 SAMPLING PROJECT PAGE 2 OF 41 TABLE OP CONTENTS PAGE TITLE PAGE................. 1 TABLE OF CONTENTS.................................. 2 INTRODUCTION ........................................ 3 SUMMARY OF AIR MONITORING ............................ 4 SUMMARY OF WIPE SAMPLING ............................. 5 ATTACHMENT 1: AIR SAMPLING RESULTS.................. . 6 SUMMARY OF AIR SAMPLING PATHSFORWARD.................. 24 ATTACHMENT 2: INVESTIGATION OF HVAC UNITS............ 26 ATTACHMENT 3: SURFACE WIPE SAMPLING RESULTS.......... 30 WIPE SAMPLING CONCLUSlONS/PATHSFORWARD....... 36 FIGURE 1: ROOF HVAC SAMPLING LOCATIONS (AIR) ........... 37 FIGURE 2: ROOF SAMPLING LOCATIONS (WIPE) ............. 38 FIGURE 3: PERCENT RECOVERY OF WIPE SAMPLES .... 39 FIGURE 4: AVERAGE VALUES AT ROOF LOCATIONS........... 40 REFERENCES.......................... 41 EID093253 E ID 093253 C-8 SAMPLING PROJECT PAGE 3 OF 41 In response io the Summary of 1995 C-8 Blood Testing (Playtls, 1995) results, and subsequent 1996 Industrial Hygiene Follow-up Report (Playtls, 1996), an extensive sampling campaign for C-8 In air and on surfaces (wipe testing) was conducted In Teflon* areas.