M22G-OIH 3M M ED ICA L DEPARTM ENT, CO RPO RA TE TO X ICO LO G Y P rotocol fo r S tudy No.

HAZLETON WASHINGTON 9 2 0 0 L E E S B U R G PIKE V I E N N A .

A lU U -o ^ o Pathology Associates International A Company of Science Applications International Corporation toEmptarm+OmmdCarmnr Sponsor: St.

AfW-ossH ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.

/IR -0 3 S 5 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: Perfluorodecanesulfonate, ammonium salt; may also be referred to as PFDS, FC-120, or 3M Sample J2905-1. (1-Decanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10heneicosafluoro-, ammonium salt, CAS # 67906-42-7) Remarks: Test sample is a mixture of the test substance in water and butyl cellosolve (approximately 25% test substance, 37.5% butyl cellosolve and 37.5% water).

/ N frU -0 5 2 .5 ACUTE TOXICITY TO FISH TEST SUBSTANCE_______________________________________________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, FC-143 or as the major component of FC1015.

y^e slsl& _ o z r i TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3).

- Olo^i ACUTE TO XICITY TO FISH (RAINBOW TROUT) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-3052. (1Octanesulfonic acid) (CAS # 2795-39-3).

AR226-3061 Study mio TOP Biocfflnpatibility Testing of S-22072.

TRADE SECRET AR226-3084 Study Title H-23004: ORAL APPROXIMATE LETHAL DOSE (ALD) IN RATS LABORATORY PROJECT ID: HL-1997-01291 AUTHOR: Tracy A.

Summary PFOS Rat Two-GenerationReproductionStudy StudyNumbers:3M T-6295.9,Argus418-008(in-lifFeA)C,T-TOX-012 (analytical).

PHYSICAUCHEMICAL PROPERTIES TEST SUBSTANCE Identity:[2-(N-Ethylperfluoroocetsaunlfonamido) ethylacrylate;may also be referredtoas B1228, D-1, ETFOSEA, or FX-13.(2-Propenoicacid,2[ethy[lheptadecafluorooctyl)slufonyl]amnio]ethylester,CAS # 423-825) Remarks:Materiiaslan ambersolid.

C 0 V AV -C@-E.@ THE DEVELOPMENT SERVICES CO#APANY Sponsors: APME Ad-Hoc APFO ToxicologyWorking Group PROTOCOL Study Mde: 26-Week CapsuleToxicityStudywithAmmonium Perfluorooctanoa(tAePFO) inCynomolgus Monkeys Date: September 23, 1998 PerforniingLaboratory: Covance LaboratoriesInc. 3301 Kinsman Boulevard Madison,Wisconsin 53704-2595 Laboratory Study Identification: ProposalNo. w6806a Covance 6329-231 Covance 6329-231 Page 2 Study 26-Week Capsule ToxicityStudy with Ammonium Cynomolgus Monkeys Perfluorooctanoate(APFO) in Purpose To assessthe effectof the testmaterialon criticaelnzyme levels,hormones, and other selectedbiochemical parameterswhen administereddailyby capsuleto cynomolgus monkeys for atleast26 weeks Sponsors APME Ad-Hoc APFO Toxicology Working Group Study Monitor Paul Lieder,PhD, DABT 3M Toxicology Services Building 220-2E-02,3M Center St.Paul, Minnesota, 55144-1000 Telephone No.: 612.737.2678 FacsimileNo.: 612.733.1773 AlternateStudy Monitor John Butenhoff,PhD, DABT 3M Telephone No.: 612.733.1962 FacsimileNo.: 612.733.1733 Sponsor's Representative David Farrar,PhD ICI Chemicals & Polymers Limited Occupational Health The Heath, Runcom, PO Box 13 Cheshire,WA7 4QF Telephone No.: 01928.513953 FacsimileNo.: 01928.515146 Study Location Covance LaboratoriesInc. 3301 Kinsman Boulevard Madison, Wisconsin 53704 Covance 6329-231 Page 3 Mailing Address: PO Box 7545-2595 Madison, Wisconsin 53707-7545 Study Director Peter J.Thomford, PhD Covance LaboratoriesInc.

BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredto as PFOS, FC-95,or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfoniacid)(CAS # 2795-39-3).

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-603EF. (1Octanesulfoniaccid)(CAS # 2795-39-3).