Document mpJYVqxpm2661bXRBDaY1o62Z

A A J2 .A 6 _ 0 2 X 3 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: OECD Guideline 203. Type: Acute static GLP: No Year completed: 1997 Species: Pimephales promelas Supplier: In-house cultures maintained at AScl-DETD, Duluth, MN. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 96-hours Statistical methods: LC50 values were calculated by the Trimmed Spearman-Karber method. The NOEC was calculated using Fisher's Exact Test (Toxstat software, Version 3.5). Test fish age: Juveniles Length and weight: Average length = 12.7 mm Average weight = 0.0165 g (wet) Loading: 0.066 g/L Pretreatment: None. Test conditions: Dilution water: Dechlorinated City of Duluth, MN, tap water (post carbon filter) that was aerated for 24 hours prior to use. Dilution water chemistry: Hardness: 48 mg/L as C aC 03 Alkalinity: 46 mg/L as CaC0 3 pH: 7.80 002329 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis and mixed by mechanical stirring. Concentrations dosing rate: Once. Stability of the test chemical solutions: Not noted. Exposure vessels: 4 L glass jars containing 2.5 L test solution. Number of replicates: 2. Number of fish per replicate: 10. Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.73 - 7.83 (control exposure) 7.31 - 7.85 (1000 mg/L exposure) - Temperature range (0-96 hours): 21.1 - 21.3 C Conductivity range (0-96 hours): 1 3 3 - 1 4 9 pmhos/cm (control exposure) 1 5 0 - 1 5 9 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96 hours): 7.1 - 9.2 mg/L (control exposure) 6.7 - 9.4 mg/L (1000 mg/L exposure) Remarks: Solutions were aerated continuously during the exposures using single bubble aeration to minimize foaming. RESULTS_____________________________________________ :__________ Nominal concentrations: Blank control, 1,130, 216, 360, 600, and 1000 mg/L Element values: 96-hour LC50 = >1000 mg/L 96-hour NOEC = 1000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS______________________________________________ ___ The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L, the highest concentration tested. Mortality observed at this concentration was 15%. The 96-hour No Observed Effect Concentration (NOEC) was determined to be 1000 mg/L. 002330 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. REFERENCES This study was conducted by AScI Corporation, AScl-Duluth Environmental Testing Division, Duluth, MN at the request of the-3M Company, Lab Request number P 258 1,1997. O T H E R ______________ Last changed: 6/26/00 002331 BEST COPY AVAILABLE AScI Corporation/AScI-Duluth Environmental Testing Division AScl-DETD Study ID# 5010-068 STUDY TITLE ACUTE TOXICITY OF FC-603EF, LIGHT WATERfTM) ATC 3%, AR-AFFF TO FATHEAD MINNOW (P1MEPHALES PROMELAS) DATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED January 28, 1997 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 . f,: / Tel. No. (218) 722-4040 ' ; . '**} 7 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068), ;; 3M Company Lab Request# P2581 BEST c o p y a v a il a b l e AScI Corporation/AScl-Duluih Environmental T oting Divmon ASclDETD Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of FC-603EF Light Water(TM) ATC 3%, AR-AFFF to Fathead Minnow (Pimephales promelas). OECD Guideline 203. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Susan Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. P2581. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Todd McGuire. Joe Amato. Donald Mount. January 21-25, 1997. Alan Mozol Report Signature^ Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. '^ " "t " BEST COPY AVAILABLE AScI Corponlion/AScl-Duluth Environmental Toning Division AScID ETD Study IDS' 5010-06 2.0 OBJECTIVE(S) To determine the 96-h LCS0 and 96-h NOEC (no observed effect concentration) of the test substance, FC-603EF, for fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 203 (OECD 1993). 3.0 TEST METHODS Test Substance Properties: FC603EF, amber-colored liquid, specific gravity ca. 1.06 (water = 1), vapor pressure ca. 15.8 mmHg, stable, and completely soluble in water. Test Organisms Test concentrations: 0 (dilution water control), 130, 216, 360, 600, and 1000 mg/L. Each test substance concentration obtained by mass additions to dilution water followed by mechanical stirring. These solutions were split into two aliquots to accommodate test replicates. Source: Healthy, juvenile fathead minnows (Pimephales promelas) obtained from AScI-DETD in-house culture. The batch of fish used in the study was hatched between December 18 and 20, 1996. Holding/acclimation: Held and acclimated from hatching until test initiation (app. 32 days) in the test dilution water. For the 48 hours preceding the test, 0% mortality occurred. At test termination, the fish were 12.7 2.4 mm in mean length and 16.5 8.9 mg in mean weight. Dilution Water ' Use in test: Used 10 organisms/replicate and 2 replicates/test concentration. `. Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. - Characteristics: At test time, the water had a hardness of 48 mg/L (as . CaC03) and a pH of 7.80. Aerated for 24 h prior to use in the test. ^ Exposure Chambers Incubation Observations Endpoint Calculations BEST COPY AVAILABLE AScI Corporation/AScl-Dululh Environmental Testing Division AScl-DETD Study ID# 5010-068 Type: 4-L glass jars. Use in test: 2.5 L of dilution water or test solution/chamber. Solutions were aerated continuously during the exposures using single bubble aeration to minimize foaming. Duration: 96 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 21.0 1C. Biological: Daily- Test organisms with abnormal behavior and signs of stress, and dead test organisms. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, DO, conductivity, and pH. All determinations were made according to APHA methods (1995). LCS0's: Trimmed Spearman-Karber Method. NOEC: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, West Inc., and University of Wyoming). 3.0 RESULTS Test Substance Toxicity (Pertinent data are in 24-h LCS0: > 1000 mg/L (Not Calculable), y Tables 1 and 2) , ,/ I s -:* J .r v- ' ' VS-A LC5q. > 1000 mg/L'(Not Calculable). ; 72-h LCSq. > 1000 mg/L (Not Calculable). 96-h LCS0: > 1000 mg/L (Not Calculable). 96-h NOEC: 1000 mg/L. . BEST COPY AVAILABLE AScI Corporation/AScI'Dutulh Environmental Testing Division ftAScl-DETD Study ID 5010-068 Total hardness (as CaC03 mg/L): 48. Alkalinity (as CaC03 mg/L): 46-52. Temperature (C): 21.1-21.3. DO (mg/L): 6.7-9.4. pH: 7.31-7.85. Specific conductivity (yjnhos/cm): 133-159. 4.0 TEST QA/QC QA/QC Criteria Data Mortality in control exposure must not be more 0% mortality. than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. The lowest DO concentration measured was 75% of the air saturation value at 2l.0C. The test solution dissolved oxygen concentrations were maintained via single bubble aeration for the entire duration of the test. Test duration must be 96 h. The test duration was 96 h. Reference Substance Toxicity Test (this test is Control chart for sodium chloride reference optional). i toxicity testing is in Raw Data Package. ; . ". .. ' ". .; v ' . S ' if . .. 5-'* 4M 4y'U$ fc BEST COPY AVAILABLE AScI Corporalion/AScI-Dululh Environmental Tolling Division ASel-DETD Study ID* 5010-068 5.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Organization for Economic Cooperation and Development (OECD). 1981/1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Hamilton, M.A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7): 714-719; Correction 12 (4): 417 (1978). West Inc., and University of Wyoming. TOXSTAT Software Package. Version 3.5. Cheyenne, Wyoming. BEST COPY AVAILABLE ASel Corporation/AScl-Duluth Environmental Testing Division AScl-DETD Study ID* 5010-068 TABLE 1. ACUTE TOXICITY OF FC-603EF LIGHT WATER(TM) ATC 3% AR-AFFF TO FATHEAD MINNOWS (P. promelas) Test Substance Nominal Concentration (mg/L) 0 (control) 130 216 360 600 1000 * Percentages are in parenthesis. Cumulative Number of Dead Organisms and Percent Mortality* 24 h 48 h 72 h 96 h 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 1 (5) 0(0) 0(0) 0(0) 1 (5) 0(0) 4(20) 1 (5) 2 (10) 0(0) 3 (15) EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS LCjo and NOEC 24-h LC50 48-h LC50 72-h LCS0 96-h LC}0 96-hNOEC Data (mg/L)b >1000 >1000 * >1000 >1000 1000 95% Confidence Interval Not calculable Not calculable Not calculable Not calculable Not applicable b LCj0 values were calculated based on test substance nominal concentrations.