Document XOynbKmBOqmKDbd2QQx5nxmoG

/Iftfc-OSIH ACTIVATED SLUDGE RESPIRATION INHIBITION TEST SUBSTANCE Identity: Perfluorooctanoic add, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-116, FC-126, FC-169, FC-143, or as a major component of FX-1003. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS #3825-26-1) Remarks: The 3M production lot number was 2327. The test sample is FX-1003. It's purity was not sufficiently characterized, though current information indicates it is a solution of <45% ammonium perfluorooctanoate^ 50% water, <3% inert perfluorinated compound and 1 - 2 % C5 and C7perfluoro analogue compounds. The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD: Methods: OECD 209 Test type: Static acute GLP: No Year Completed: 1990 Analytical monitoring: Dissolved oxygen concentrations. Stock and test solution preparation: Test substance solution was created by mass addition to a solution containing 284 mL Millipore Milli-Q water, 16 mL synthetic sewage feed, and 200 mL inoculum. A reference solution was created using a 500 mg/L stock solution of 3,5dichlorophenol. Inoculum source: Mixed liquor activated sludge collected from Metro Wastewater Treatment Plan, St Paul, MN Mixed liquor Suspended Solids: 3.2 g/L Inoculum holding: None - used day obtained. Test Conditions: pH of Activated Sludge Mixed Liquor: 6.7 (initial) and 7.1 (final) pH of 1,000 mg/L test concentration: 7.3 (initial). Temperature: 2 0 -2 1 C Element basis: Respiration inhibition as determined by oxygen consumption. RESULTS Nominal concentrations: Bk control, Reference control, 1000 mg/L 003873 Element values: 30-minute EC50 = >1,000 mg/L 3-hour EC50 = >1,000 mg/L All element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. CONCLUSIONS The FX-1003 3-hour EC50 for activated sludge respiration inhibition was determined to be >1,000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY ____________________________________ Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. The testing procedure was also not fully documented. REFERENCES This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number G2882. OTHER Last changed: 5/25/00 C03S74 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR LR- S l J L IIS jS L Date < 2 - U o ~ < iL Analyst Type T e s t ) > A c A -w J O c te A - S W i ^ e . fc>pirdcUov\ Xr>Ui b. ^ Source^ A & r o L U a J x te ' . 1r e < M m e n + _ H < l fa # Date Obtained A - ) lo ~ & Date Used ^ ~ j b " O Mixed Liquor Suspended Solids (MLSS) 2s. g/L Adjustment g/L pH UThT-vi'a-t- >.7 fi'n o f - 7./ Comments _______ *_____________________________________ _________ Test Substance Sample Description F X ~ j Q 0 3 > L o ^ t '?>3t ~7 Sample pH JpbO }Lj r 7 . 3 Adjustment n & r \ Comments_______ ______ ____ ___ _______ Reference Substance Sample Description 3 . - T ) c M o r n ' p U e n 1 Sample p H ^ k Salvi l 7,5" AdJustment J Comments & > o y y \A - ) L - Exposure Conditions Test Duration 3 - Mnu L i. Test Temperature t o - g l I C Coment I S if t- ___ Pirn___ l o M-U . i g II2 Z V 003875 Fon 10820-ft-O-PW O Sample Description Px- 1003 oi 7 Lab Request No.j^2-i2i5L^2 Environmental Lab -- Work Sheet OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 Pafle Date: ^ of ----- & ~lb~ tfp Analyst: Jest .Solutiim.sJbrfiparat.inn. Test Cone, mg added .mlmq-water ml syn. sewage ml microbial /ooo - ZZH S^e>o + 8 H +- \u f -t- _____ Ue2____ +~ DO .z o o Total volune ml - So-a x __pfj.T e st So)n, - __ Z-3_______________ __S& t g>ubdtmce gtppa.fewfcly__ m \T i big... wv? gJtefr:, 0.am..roiS__^tiaVQrL LQ ti l a z y 003876 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST. #209 CALCULATION OF RESULTS Test Substance. F X - /D0 3 _______ Loy j t 3 P 7 ______ 1 30 - MINUTE EXPOSURE Test Substance Concentration (mg/L) ID O Z> Oxygen Uptake Rate R O . h<4&5- 0 .u 1q 4 R Corrected^ to 20 C 0 . y &~ 0 .u 1q 4 I.R Date Analyst^ f r T " % Inhibition in Respiration Rate1^ -- <?. 1 . O ' 7 1 . ^ 1 ____ 0 7 1 3 e? Percent Difference in Controls^____ ^ ,,9 EC50 (951 Confidence Limits) TMg/T- ^ /)<0 0 Test Substance Oxygen Uptake Rate Concentration (mg/L) R KSlHiHHiliB O (0 234 JO O O 0 .. s s ^ -- R Corrected^ to 2CfC 1 D .5 S o % Inhibition in ,2-\ Respiration Rate1^ - 17 CONTROL II O 7 0 . ^ 0 ___ 0 - 7 / 4 Percent Difference in Controls^ ECSQ (95% Confidence Limits) mg/L 8 9o ,,Q___ -- (1) Temp. Corrections ' x (mg 02/L-min.) K _ lj4 (2) I Inhibitions* < ' X 100 K + RCj Where: R * Oxygen uptake rate (mg 02/L-min.) T,obs Sample temp.,*C L & * 1.047, x - T,obs - 20*C C Control (3) I Difference-s 1 1 -- X 100 (RC, RC\ fS j The two control respiration rates should be within 151 of each othe ^ 6* ' 4 - -=>1- ~fSrf So In. p y CQ3S77 a-o-nvo G *SS 3Lab Request No- ^ ^ Sample Description 3. S- tJi'cW orophenol fia-erente. Sufes+anceL >e*X Uvb<M 0 + V 7 - &fO-C> Environmental Lab --Work Sheet OECD ACTIVATED SLUDGE RESPIRATION DfHBITION TEST #209 Pafle ^ of -- Date: ^ ~ ,, Analyst: S4fg>_____ Stock and Test Solutions Preparation STOCK SOLUTION: , 5 ^ r v ^ 3 , 6 ' ~ b c f > * IQ m l I ^ A J A / * O H _______________ T b ) u t e d - t o . 3 g m ^ , tfVy. > V W l i j - Q u ; A s | r __________ ______________ A A e J U N t u v \ A e r S f r r ii/u ^ J r o p w lS C . onrf 1 Ihci^igM-fc f p _________l^rluVrL-4z> 6ne, l i i e r toH-U /Atill - O Loader_____ ____ __S t ocfc----Soln.,_ p H r 7.~_____ __ ______: ______ v d u , l./D indi - _O -S _'i . S '- b c P Test Cone. ml stock soln mlMQ water ml syn. sewage ml microbial mg/L ^imi im p B iilP M W ^HMSnii2LiSLwM^ I***. gnKQQ -- ___________ t - lb . * Zoo 10 IQ I- A m 4- /, * 2 o o Total volume ___ ml____ SO S C03878 lWlHWlMAL LABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST. #209 CALCULATION OF RESULTS Test Substance-- --t3y,^5- 'ibiVJkUro ?Vqx\A ________ 30 - MINUTE EXPOSURE Test Substance Concentration ime/L) CONTROL T 1D Oxygen Uptake Rate R O. R Corrected^ to 20 C o UHBST > I.R ^ Pat Analyst' ' \ Inhibition in f Respiration Rate'-2 -- HA CONTROL II 0 . 7 I Z H _____ 0 - 7 1 2 4 Percent Difference in Cant.rolsi" ECjjq (951 Confidence Limitcl mo/T. 3 - m m rtposurf. to % yy\cllL O Test Substance Concentration (mg/L) Oxygen Uptake Rate R u rL u c e A 1 R. inViib, R Corrected^ to 2(fC % Inhibition in Respiration Rate^ BliiVHHIHi - /o O' o , a \ D <=i o. L>S<P^ O - 9.DIH 70 * .- *' '** ga5BBL.il_________ - 7030 O -U T lH --- Percent Difference in Controls^ ___ ____________2_-__ ECS0 (951 Confidence Limits) mg/L-- f O L /.hdLu.Cs&-.7 .P ?k.. ItlVi t.lp,- (1) Temp. Corrections (mg o2/L-min.) RC, (3) % DifferenceJ 15?i X II (RC, RC\ M il (2) Inhibition* / .- - t * x 100 rry The two control respiration rat should be within 151 of each otl (5 . ; Kj Where: R - Oxygen uptake rate (mg 0-/L-minJ T,obs Sample tanp.,*C c - 1.047, x - T,obs - 20l*C C Control See- P% i S o r 'te S '*- p rep i C03S79