AR R26 33S 497 ---- --_ AR226 3315 suptenents bony TRADE SECRET Study Tide 90-Day Gavage Study iHn2R5at4s25wi:thSOunbec-hGreonneircaTtoixoinciRteyproduction Evaluations Supplement 1 Laboratory Project ID: DuPont-11418 TesT GUIDELINES: OUSP.PTESPA87H0e.a3l1t0h0E(fAfUecGt-s1T9e9s8t)Guidelines -- AumOR: Susan A.

onciusions rrom rrevious Keview (15-bec-2003) AR226-3325 bOE Used to Screen Main Ef PFOA Levels I DuPont Confidential onpanystant.Doos 7!

AR226-3369 fttAT^X-EFFKCT FR ^ALYTIC-AL METHODS FOR UETERPjtiNA-nOM OF PEFIFLUOH^ATEO CARBOKVLiO ACIDS iK EftiVtRONMENTAt ASiD EEOLOGECAt SAMPLES Cliarias ft. fowloy', Ttmothy W. ttysa' nnd Robflrt C.

CAS Number 1763-23-1,29081-56-9,70225-14-8,2795-39-3,29457-72-5 /3M Company 4/27/2000 Voluntary Use and Exposure Information Prof*de PerfluorooctaneSulfonicAcid and Various SaltForms I.

@77P7R1= PathologAyssociatelsnterriaatlion A Company ofScienceApplicatioInnsternatioCnoarlporation AnE"p"-O*MdCoxMwWy CollProliferaRteipoonrt 4-WeekCapsulTeoxiciSttyudyWithPerfluoroocStualnfeonic Acid PotassiumSalt(PFOS; T-6295)in Cynomoigus Monkeys Covance StudyNumber 6329-222 QUALITY ASSURANCE STATEMENT Thiscellproliferatipomnjecthas been inspectedand auditedby thePAI QualityAssurance Unit(OAU) as requiredby the Good LaboratoryPractice(GLP) regulationpsromulgatedby the U.S.Envimnmental ProtectioAngency.

3M EnvironmentaLlaboratory FinalReport-AnalyticSatludy Single-DoDsermalAbsorption/ToxiSctiutdyyofT-6053inRabbits In-VivSotudyReferencNeumber:HWI#6329-137 StudyNumber: AMDT-022195.1 TestSubstanceF:C-99(T-6053) Name andAddressofSponsor: 3M SCD Division 367 Grove Street St.Paul,MN 55106 Name and Address ofTestingFacility: 3M EnvironmentalTechnology& Services 935 Bush Avenue St.Paul,MN 55106 Method Numbers AMDT-M-1-0, AMDT-M-2-0, AMDT-M-4-0, AMDT-M-5-0, AMDT-M-8-0, and Revisions: Thermal Extractionof Fluorideby Means of a Modified Dohrmann DX2000 OrganicHalideAnalyzer-Liver FluorideMeasurement by Means ofan Orion EA940 Expandable Ion Analyzer ExtractionofFluorochemicalsfrom RabbitLiver AnalysisofRabbitLiverExtractforFluorochemicalsUsing ElectrospraMyass Spectrometry Analysisof FluorideUsing the SkalarSegmented Flow Analyzer with Ion SelectivEelectrode InitiatioDnate: See attachedprotocol Author:James D.

NOTOX Safety& Environmental Research LV.

BIODEGRADATION TEST SUBSTANCE Identity:Perfluorooctanesulfonylamido(ethyl)acetmaatye;alsobe referredtoas PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-1 09,or FC-109-X.

TOXICITY TO AQUATIC PLANTS (e.g.A,lgae) TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium salt,Ammonium perfluorooctanoateP,FO, FC116, FC-126, FC-1 69,or FC-143.

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorooctanoiaccid;may also be referredtoas PFOA, FC26, or FX-1001.

BIODEGRADATION (OECD 301E) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95,oras a component ofFC-203A. (1Octanesulfoniaccid)(CAS # 2795-39-3).

MICROBICS MICROTOXO TEST SUBSTANCE TOXICITY TEST Identity:Mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component ofFC-203CF-X or FC-203CF. (1-Octanesulfoniaccid)(CAS # 2795-39-3).

MICROBICS MICROTOX@ TOXICITY I'EST TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3017. (1Octanesulfoniaccid)(CAS # 2795-39-3).

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COVAW'C-E-'-*'\ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St.Paul,Ntinnesota FINAL REPORT Study Title: 13-Week DietaryToxicityStudywithN-Methyl Perfluorooctanesulfonamido Ethanol(N-MeFOSE, T-6314)inRats Author: PeterJ.Thomford, PhD Study Completion Date: June 30,2000 PerformingLaboratory: Covance LaboratoriesInc. 3301 Kinsman Boulevard Madison,Wisconsin 53704-2595 LaboratoryStudy Identification: Covance 6329-225 Sponsor Study Identification: StudyNo. 3M T-6314.1 Page I of560 QUALITY ASSURANCE STATEmyNT Covance 6329-225 3M T-6314.1 Thisreport,withtheexceptionofAppendix 7,hasbeenreviewedby theQuality Assurance Unit of Covance LaboratoriesInc.