Document ppd3kY05q2dVY6p87q95z0jNd
MICROBICS MICROTOX@ TOXICITY I'EST
TEST SUBSTANCE
Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3017. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-3017. CurrentinformationindicatesFC-3017 isa mixtureof 0.67% PFOS, 20% diethyleneglycolbutylether,55.41% water,21% ethyleneglycol, 1.33% Hydroxy foamer, 1% sodium octylsulfate0,.5% polyoxyethylene monooctylphenylether,0.04% sodium laurylsulfatea,nd 0.05% tolyltraizole.
The followingsummaty applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately retlecttoxicityof the fluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotox@"BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1995 Species: Photobactetiumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period:30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@ Test organism source: Microbics'Reagent. Test conditions:
Diluent: MilliporMeilli-Q"wmater Test temperature: Not noted. Stock and testsolutionpreparation: A 2000 mg/L primarystock solutionwas prepared,.bdyissolving200 mg oftestsubstance in100 mL ofMilliporMeilli-Qwater. The pH ofthe stocksolutionwas 6.0, and an osmotic adjustmentwas made using200 mg NaCi dissolvedin 10 mL stocksolution.Appearance oftestsolutionswas noted as "cleae'.Alltestsolutionswere made by proportionadlilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS
Nominal concentrations: Blank control,125,250, 500, and 1000 mg/L
Element value:
5-minute EC50 = > 1000 mg/L 15-minute EC50 = 785 (728 - 846) mg/L 30-minuteEC50 = 558 (541- 574) mg/L
Element valuesbased on nominal concentrations.
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 15-minuteEC5()forPhotobacteriumphosphoreum was determined tobe 558 mg/L witha 95% confidenceintervaolf 541 to 574 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
Reliability:Klimischranking= 2. Testingmeets allcdteriaforqualitytesting. However, sample puritywas not propedy characterizedand itlacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
Thisstudywas conducted by the3M EnvironmentalLaboratory,St.Paul,MN, Lab Request number N3188, 1995.
OTHER
Last changed: 6/26/00.
ic
EN-VIROiNNtEN*TAL LABORATORY:
NtICROTOX PREP STEEET
LR: Date: Analyst:
TEST SUBSTA.NCE:
1-7
1. PreparationofStock Solution
*,VfassIVolT.estSubstance:
*Final DilutionVolume:
IL
Diluent. @l'!
FinalConcentrationofStock Solution:
2.pH Adjustment of Stock Solution
InitiaplH ofstockSolution: *pHAdjustedto: pH.4djustedwith:
3.Osmotic Adjustment of Stock Solution * OsmoticAdjuster. * Comments:
4.AAp e2rance ofpH and OsmoticallyAdjusted Stock Soludon or OPAQUE (circloene)
* Color. * Comments:
S.Comments
Mli'r@oT(-,XDATA riE!',-)RT
TE-,!' --IATE:
AL)Prov@-(j'uy-
BA37(' mg
rt: riu L; I Cj/TT
L@3. 4@-
7P
43.:j8 43. 0--i
5 1),o 00!;
--------------------
7
4
EC50
.10 LZODDL)I)I)DFOI)DDDDDD@1)1P'.,DjD'D)DDDDD^DDDDDDDDDDD- DDI)DDDDDDPIDl)f)!'@T!DDI)Y
10()
1000 CONCENTRATION IOE4
IOE5
EC501304.9501
(95% CONFIDENCE PANGE:1116.0963 To 1525.7596) f,-)rcalculati-,@,113
AF'T@A 4 A,, i'@
I)A'FA
A i',
13AMMA
i;:.'T
"Mil
. .... .... .... ......... ..
0. 34:);' 0 .7 4;'0, 0.8116
i8 e(i.3746 Ci.3746
C). 75Po 0.7560
E, 4 4 e, '-',14 :'E, ;"14.'L-@ 42. 5"8 44.98.-)
"OtiF7DENCE RAN('jE:1116 0963 TO
i4:i @.8 Ki F, Ml(-RC.@TOX DATA Fi,'-30,
M P,F i,
TE@*,T DATE:
TEST TIMF:
Appr,
l,y:
Os mot.ic Ati I)ilution Ci:@nc-nti-at
GA"MA
31
z
-14
-- -- - - - - -- -
4
10 eDi@PIl@l;L)DDI@DL;I)Df)DD. D- J)DDDDDDDDDDDDDDDDDD-
100
1000 CONCENTRAT10N
3
3
DL-)DDDDDDr@-r)DDDDDr)DDDY
lOE4
lOE5
EC50 784.8150 e95% CONFTDENCE RANGE: 728.4058 TO '845.5927) 3$ [)sed for ca'----tilatioris
STATISTICAL REPORT FOR DATA FILE N3 188.Kl5
ESTIMATING EQUATION: LOG C 0.9025 x I,OG b +2.8948
NUMBER OF DATA -PAIRS USED: 6
RESIDUAL VARIANCE::'-" 0.00050
-95%.CONFIDENCE FACTOR: 1.07744 FOR ECSO
CORRELATION COEFFICIENT: R
0.99724
COEFFICIENT OF DETERMINATION: R-2
0.99450
-EFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE):
EC50 = 784.8150,mg/L EC20 = 224.6047 mg/L ECIO = 108.0389 mg/L ECOL = 12.4091 mg/L
(728.4058 TO (203.0035 TO ( 91.'-'765TO
8.4346 TO
845.5927) 248.5043)*** 127.8797)***
18.2565)***
EXTRAPOCATEE) RESULTS
W4 1,;
-7c,@
INITIAL DATA
NUMBER
1 2 3 4 5 6
CONCENTRATION
125.0000 125.0000 250.0000 250.0000 500.0000 500.0000 1000.0000 1000.0000
GAMMA@
o.i 0.092,.-., 0.296 0..277-
@o 1 '235
N31 8 8 Kl5
%-EFFECT
.10.53 8.47
22.85 21.69 37.20 37.12 55.26 .58.50
9.50 Z2.27 37.16 56.88
,t[[[T6udh SPACE BAR to continue.
',@:FINAL RESULTS
-N3188.Kl5
I WHHNMMMMMHMMMMMMIYMMMmmmmmlimwl
-'NMUB ER"''
CONC.
GAMMA
':GAMkA-,'E
-------- ---------
FLTLCT
250.0000
0.2961 ',.t,-: - 8 7@:;% 2
-:'-'-,-'-2-5o0oDO
0.2770,:@-1.@.@
500@10000'@"@ 0. 5923',@@@,
.'@,n,DUG-0000
0,.5 9 0 3
6
12?,@0;t
0
1 2352
1.4094
95% CO NFIDENCR:,;@',
(c)
MICROTOX DATA REPORT-
FILE NAME:@N3188.K30
TEST t)ATi.'
TEST TIME: t1L!
Investigator:.
Approved by:
Sample-Des,cription: FC-3017
Procedure:'BASIC
-6,Smotic AdJu5tm'ent:
initial Concentration -1000 mg/L
Dilution Factor : 2
T".st Time: 30 minutes
iconcentration Units
NUMBER
IO/IT -----------
CONC.', ------
GAMMA ----------
1
125.
2
85.85/'42.15
125.0000'
0 i4720'
3
87.73/ 35.36
250,@0000@
0.'397440.
4
86.74/ 34.98
250.000G@-:-
0.39668$t
5
85.51/@25.79
500.0000@@;...,,, 0.86750#
6
83.45/@25.52
500.0000
"0.84180#
7
80.15/ .15.18
1000.0000
1 97391#
8
76.77/ 14.21@
1000.0000
2.0429401,
IT/IO 53.44/ 95.42 52.52/ 92.72**
RATIOS
0.5601
0.5664*,@
CORRECTION FACTOR
0.5632
NaCl'' mg/L:@
10 ZDI)
3
G A 3'M3
3 A 3,@
)D !DQDDD.DDL)DL@l.'D.DDDDDDDDDDDDDDDDDDDDDDDFPDD? -3 3 3.'
..
7
3
r
P5
STATISTICAL REPORT FOR DATA FILE N3188.K30
ESTIMATING EQUATION" LOG C 0.8541 LOr. b +2.7464
NUMBER OF DATA PAIRS USED: 6
RESIDUAL VARIANCE: 0.00012
95% CONFIDENCE FACTOR: 1.02998 FOR EC50
CORRELATION COEFFICIENT: R
0.99932
COEFFICIENT OF@ DETERMINATION: R-2
0.99864
EFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE):
EC50 = 657.7067 mg/L (541.47f,2 TO 574.4247)
EC20 = 170.681.9 mg/L (160.3766 'LPO 181.6493)***
ECIO = 85-3864 mg/L
77-6362 TO 93.9104)***
ECOL = 11.0138,mg/L,,.
9.0374 'rO., 13.4225)***
EXTRAPOLATED RESULTS
INITIAL DATA
NUMBER
CONCENTRATION
1
125.0000
2
125.0000
3
250.0000
4
5
500.0000
6
500.0000.
7
1000.0000'
8
looo,.
oooo,.:,.:4;
-,7
GAMMA*
'397 86
0.842 1.974
13
N3188.K30
X EFFECT.,
14.27 12.83:@, 28.44 28.40 46.45 45.71 66.37 67.14
13.55 28.42, 46'.08 66.76 ,
-'7 77A',@"-Ik-@
4,
oo
0
oj
N3188.K30
EFFECT-,,@
?-8 440 91
l@' A-^" A @3 ',41@'705 @;
-;374