Document EqQDGK8Xv51vnpZd4w8vvqvej
MICROBICS MICROTOXO TEST SUBSTANCE
TOXICITY TEST
Identity:Mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component ofFC-203CF-X or FC-203CF. (1-Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 101. The testsample isFC203CF. Currentinformationindicatesitisa mixtureof 1% PFOS, 20% diethyleneglycolmonobutyl ether,70.2% water,2.75% Acrylicfoamer, 3% sodium octylsulfate,1% triethanolamine2,% sulfonatedalkylether salts,and 0.05% tolyltriazole.
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotox@)"BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1991 Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@ Test organism source: Microbics'Reagent. Test conditions:
Diluent: MilliporMeilli-QTM water Test temperature: Not noted. Stock and testsolution preparation: A primary2000 mg/L stock solutionwas prepared'b.y dissolving200 mg oftestsubstance in100 mL of MilliporMeilli-Qwater. The pH ofthe stocksolutionwas 6.6. An osmoticadjustmentwas made using 200 mg NaCi dissolvedin 10 mL stocksolution.Appearance oftestsolutionswas noted as "clear, colorless"A.lltestsolutionswere made by proportionadlilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS
Nominal concentrations: Blank control,125,250, 500, and 1000 mg/L
Element value:
5-minute EC50 = 470 (420 - 520) mg/L 15-minute EC50 = 360 (330 - 390) mg/L 30-minute EC50 = 280 (260 - 300) mg/L
Element values based on nominalconcentrations.
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 30-minuteEC50 forPhotobacteriumphosphoreum was determined to be 280 mg/L witha 95% confidenceintervaolf 260 to 300 mg/L.
Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking= 2. Testingmeets allcriterifaorqualitytesting. However, the sample puritywas not propedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
This study was conducted by the 3M EnvironmentalLaboratory,St.Paul,MN, Lab Request number J1884-3, 1991.
OTHER
Last changed: 6/28/00.
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EWRONMENTAL UU30RATORY MICROSICS MICROTOXO SYSTEM:"
'TO@iC[TY TEST RESULTS:
PERCENT UGHT. LOSS:';,@
@,VVITCOLATE TRITON X40
FM 4400',,
AAW U;M2A-AM
1,= Jidda AROW A&"
12. 11
25 31.2 5
125:0 250940-1
60
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0
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oi
46Vt "IWO a*
63 85 i@'ll,
14 17 22 111 i1 22 It'@-4'04
in@ I'@ptm*law
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of
TEST SUBSTANCE:
TEST DAT E: 7/1-9 Iq I
ANALYST:
SAMPLE CUVETTE
LFMAT-XMY
CONCBffRATION
DUP=TE
RAW D
4o# )6
INITIAL@,-
FEADM
-5-Miii
17
NAL READWO s
is-mIN 1 (151
30-MIN 1 (301
3,X
33
.2q
IC&-
., )mp
pioil
MICROTOX (r). DA
T
iri wxp.:@ jle84 -3
CONC
,2
4,. ,
'.6,
BLANK BLANK
125.000 125.000 250-000250-000 500.000 500.ooo %1000.00 0 %iooo.oo 0
Bo/Bt- 99 RATIO- 0.5758
lo/It
93.0/ 45.0 96.0/ 43.0, 98.0/ 36.0 91.1 D/ 35. o 92.0/ 26.0 84.0/ 23.0 '',94.0/ 17.0
98.0/ 17.0
.57
0.190 iD.28 5 0. 567
0 497
l.'0371.,103
' 2.319
a-ES T L
0.240 0.240 0.507 0.507 1.075 1.075
2.277 2.277
EC 50
465.422
0.99110
SLOPE
417.172 TO 519.253."@-)
il7O @41.70-5.20) ryitIL
0.9071
INTERCEPT
@+6.,1429
RES.
PROB..
0 195
100
2- 0 15 5
63
0 los,
14'
34
2x4
34
0>
0;.167@,
74
0--'L73",
------------ V--
33
1 @!:@'3,5-
-
51
70
70
x /cly
-8
P6 3 o@i/-
MICROTOX(R) DATA SHEET,@ 3C Lot'101 15-min emp. J1884-3 7/19/91 %ab
PAIR
CD'NC.
1
125.000
2
125.000
250.000
4
250.000
5
500.000
6
500.*Ooo
7
%1000.000
8
%looo.000
BLANK @o/Bt- 99 BLANK RATIO- 0.4040
lo/It
93.0/ 30.0 96.0/ 29.0 98.0/ 23.0 91.0/ 22.0 92.0/ 15.0 84.0/ 14.0
94.0/ 9.0 98.0/ 10.0
40
A-OBS
0.253 0.338 0.722 0.671 1.478 1.424
3.220 2.960
EC 50 '-.360 954
R -0.99529
SLOPE
334.846
330
- 0.8792
TO V89.097
3 'ID
INTERCEPT
+5.8887
O-EST
0.303 0.303 0.661 0.661 1.443 1.443
3.152 Z.152
Lcss (1)
1?3
goo
o
511
7&0
76
7-6-
foc> (i6-c6s
-r*l
:2 3
RES,.' PROB.
0.138
0 0.07llz,
too,
73
33
.0
.0
082'*-- 35, 84
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ketered Trade' ria
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PZ6
FC-203CF,'Lot 101
kICROTOX(r@@ 30-min exP.
DATA SHEET 7/19/91 sab
PAIR
.,2 3. 4
6 -.7 8
BLANK BLANK
'CONC.
125.000 125.000 250.000 250-000 500.000 500-000 %1000.000 %1000.000
Bo/Bt= 99 RATIO= 0.3333
lo/It
93.0/ 23.0 96.0/ 23.0 98.0/ 16.0 91.0/ 26-0 92.0/ 10.0 84.0/ 9.0
94.0/ 6.0 9e.o/ 6.0
33
G-OBS
0.348 0.391 J.042 0.896 2.067 2.111
4.222 4.444
EC 50 - 276.232
E:r -
, R -0,.99632 SLOPE
257.632
so q
0.8431
TO 296-174 INTERCEPT,.-
G-EST
Locs
00..339944 is 17
0.891
51
0.891
47
2.015
2.015
11
4.558
4.558
OIL
Aleo
RES. 1 0. 1 Is .-;,.2 0. 111
0.069 0. 073 0 073 -'0'?.074
@'O.106 @,@,@o.109
PROD. 100 89 34 3e 39
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86
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