3M Environmental Laboratory R126-0005 M ethod A nalysis of Potassium Perfluorooctanesulfonate or O ther Fluorochemicals in Serum Extracts U sing H PLC-Electrospray/M ass Spectrometry Method Number: ETS-8-5.1 Author: Lisa Clemen, Robert Wynne Approved By: 'L l Laboratory Manager Adoption Date: 03/01/99 Revision Date: <V / 2 C / Date Group Leader Technical Reviewer Date dh i / m Date 1.0 S c o p e a n d A p p l ic a t io n __________________________________________________________________ 1.1 Scope: This method describes the analysis of serum extracts for fluorochemical surfactants using HPLC-electrospray/mass spectrometry. 1.2 Applicable Compounds: Fluorochemical surfactants or other fluorinated compounds, or other ionizable compounds. 1.3 Matrices: Rabbit, rat, bovine, monkey, and human serum, or other fluids as designated in the validation report.

ENVIRONMENTAL PHYSICAL/CHEMICAL, FATE, EFFECTS AND MONITORING STUDIES BACK TO COMPOUNDS STUDY TITLE LABORATORY OR DATE AUTHOR COMPLETED TYPE Physical/Chemical Properties Impinger Studies of Volatility of FC-95 and FC-143 3M Environmental Lab Characterization Study of PFOA: (LOT #332), Primary Standard-Test Control Reference #TCR-99030-030.

BACK TO MAIN ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE_____________________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-116, FC-126, FC-169, or FC-143.

ARM-Ool'l Study Protocols/Proposals of Ongoing Research 1.

nc-oio-j CHRONIC TOXICITY TO EARLY LIFE STAGE OF FISH TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as 14C78.02, PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, potassium salt, CAS #2795-39-3) Remarks field: The test substance is a white powder.

HAZLETON LABORATORIES AMERICA.

AR 1%-oiGi REPORT AMENDMENT NO. 1 TWO YEAR ORAL (DIET) TOXICITY/CARCINOGENICITY STUDY OF FLUOROCHEMICAL FM-3924 IN RATS Experiment NO.0281CR0012 The attached pages include revisions in the first paragraph on page 3 of the Summary and in the third paragraph of page 22 of the Discussion.

418-011: PAGE F-1 M W rO Z rt FINAL PILOT REPORT Study Title Oral (Gavage) Dosage-Range Developmental Toxicity Study of N-EtFOSE in Rats Sponsor's Study Number: T-6316.7 Author Raymond G.

ARX&0357 CHRONIC TOXICITY TO FISH TEST SUBSTANCE Identity: N-ethylperfluorooctane sulfonamidoethanol; may also be referred to as N-EtFOSE Alcohol or FM-3422. (1-Octanesulfonamide, N-ethyl1 I 1I2 , 2 I3 ,3 )4 ,4 ,5 ,5 ,6 I6,7,7,8>8I8-heptadecafluoro-N-(2-hydroxyethyl)-, C A S# 1691-99-2) Remarks: Material is an off-white, waxy solid of uncharacterized purity.

BIOACCUMULATION (ANALYTICAL ONLY) TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, FC-116, FC-126, FC-169, or FC-143.

M <6 - 0 7 5 8 EFFECT OF DEGRADATION ON FOAMING TEST SUBSTANCE________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203A or FC203 NFP. (1-Octanesulfonic acid) (CAS # 2795-39-3).

M AA6 _ O7/-S' ACUTE TO XIC ITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-203C and L-5440. (1-Octanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, potassium salt, CAS # 2795-39-3) Rem arks: The 3M production lot number was 501.

A R M 6 - 07S X ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3).

M 356 ~Z0 7 BIODEGRADATION (OECD 301E) TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3).

* 6ft Aazi6_ ior*> EPA / SPI FMG Meeting January 30, 2002 Agenda Introduction Toxicology/PK Program Studies underway Progress Time line r-o cr-i cr O -n c\n ro r ,3 C.O ' rn Environmental Effects/Fate Testing program Environmental distribution Summary I PROJECT SUMMARY REF: APFO - 2 SPONSOR APME PTFE Committee Members and APFO Suppliers (Asahi Glass, DuPont, Dyneon, Daikin, Ausimont, Solvay, AtoFina 3M, Miteni) CHEMICAL Ammonium Perfluoro-octanoate (APFO - FC143) STUDY Preliminary 4 week oral toxicity study in Cynomolgus monkeys. 6 month oral toxicity study in Cynomolgus monkeys to include an additional 13 week recovery period .