Document 9JpKeyooDQra6zzRr7dyoxRaR

BACK TO MAIN ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE_____________________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: The 3M production lot number was 427. The test sample is FC-143, referred to by the test laboratory as N2803-4. The T.R. Wilbury study number is 895-TH. The purity of the sample was not sufficiently characterized, although current information indicates it is a mixture of 96.5 - 100% test substance and 0 3.5% C6 , C7, and C9 perfluoro analogue compounds. METHOD Method: U.S. EPA-TSCA Guideline 797.1400 Type: Acute static GLP: Yes Year completed: 1995 Species: Pimephales promelas Supplier: Aquatic Biosystems, Fort Collins, CO Analytical monitoring: DO, conductivity, pH and temperature Exposure period: 96-hours Statistical methods: LC50 values calculated, when possible, by probit analysis, moving average method or binomial probability with non-linear interpolation using the computer software of C.E. Stephan. Test fish age: Not noted. Length and weight: Average length = 28.6 cm Average weight = 0.19g (wet) Loading: 0.13 g/L Pretreatment: None Test conditions: Dilution Water: Deionized water adjusted to a hardness of 40-48 mg/L as CaCO3/L Dilution water chemistry: Not noted. Lighting: Cool-white fluorescent bulbs with an intensity of 30 ft-c. Photoperiod of 16-hours light, 8 -hours dark with a 15 minute transition period. Stock and test solutions preparation: Test substance added directly to the dilution water in the test vessels. Concentrations dosing rate: Once Stability of the test chemical solutions: No insoluble material was noted during the test. BACK TO MAIN Exposure vessels: 20 L glass aquaria containing approximately 15 L of test solution, water depth approximately 18 cm. Number of replicates: 2 Number of fish per replicate: 10 Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 96 hours): 7.2 - 8.5 mg/L (control exposure) 6.7 - 8.5 mg/L (1,000 mg/L exposure) Conductivity range (0 - 96 hours) 140 - 180 pmhos/cm (control exposure) 280 - 380 pmhos/cm (1,000 mg/L exposure) pH range (0 - 96 hours) 7.3 - 7.6 (control exposure) 7.4 - 7.5 (1,000 mg/L exposure) Test temperature range (0 - 96 hours) 22.0 - 22.6C (control exposure) 21.8 - 22.5C (1000 mg/L exposure) RESULTS Nominal concentrations: Bk control, 160, 250, 400, 630, 1,000 mg/L Element value and 95% confidence interval: 24-hour LC50 = > 1,000 mg/L (C.I. not calculable) 48-hour LC50 = 790 (630 - 1,000) mg/L 72-hour LC50 = 760 (630 - 1,000) mg/L 96-hour LC50 = 740 (660 - 830) mg/L 96-hour NOEC = 400 mg/L Element values based on nominal concentrations Statistical Evaluation of Mortality: The 48 and 72-hour LC50 values were determined by binomial interpolation. Probit was used to calculate the 96-hour LC50. Biological observations after 96-hours: Fish in the control and the 160, 250, and 400 mg/L exposure concentrations appeared normal. Dark discoloration and erratic swimming of two fish was observed at the 24hour observation period in the 1,000 mg/L exposure concentration. BACK TO MAIN Cumulative percent mortality: Mean Measured 24-hours Test Conc., mg/L 48-hours 72-hours Control 0 0 0 160 0 0 0 250 0 0 0 400 0 0 0 630 0 10 20 1,000 15 90 90 96-hours 0 0 0 0 25 90 Lowest concentration causing 100% mortality: None Mortality of controls: None CONCLUSIONS________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be 740 mg/L with a 95% confidence interval of 660 -8 3 0 mg/L. The 96-hour no observed effects concentration (NOEC) was 400 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking = 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. REFERENCES This study was conducted at T.R. Wilbury Laboratories, Inc., Marblehead, MA, at the request of the 3M Company, Lab Request number N2803-4. OTHER_________________________________________________________ Last changed: 4/01/01