/75 7 International, Ltd.----------------------------- _______Project Number 454A-102 PFOS: A 96-HOUR STATIC ACUTE TOXICITY TEST WITH THE FATHEAD MINNOW (,Pimephales promelas) ADDENDUM WILDLIFE INTERNATIONAL, LTD.

AR23-Ot(& PFOA and Associated Salts Synonyms: FC-26, FX-1001, PFO, FC-143, 78.03, FC126, N-2803-3, FX-1003, FC-118, FC-1015, FC-1015-X Physical/Chemical Properties Title Laboratory or Author Date Type Completed Water, acetone and toluene solubilitiy estimates - FC-143 3M Env.

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AJP003728 rrH* OQHW BEST COPY AVAILABLE COMPUTATION SHEET USE STANDARDS FOR MINIM UM ESSENTIA!.

AR226-2041 To: Partick J Cavanagh/Cl/DuPont@ DuPont cc: Roger J Zipfel/AE/DuPont@ DuPont S u b ject 6/9/99 C -8 Sam pling Pat, Samples were taken today from 1:30 PM to 6:00 PM in the following locations: inside the C-8 recovery building inside the C-8 recovery building control room recovery building roof smoking area roof area outside lunchroom The wind conditions were 3-5 mph out of the north at the start of sampling and out of the south/east at the end of sampling.

* r AR226-2445 ZIPFEL 5 08 /04/97 03:51 PM To: WEBERDA , ELLISDE A T A1 AT CSOC cc: STEWART , HARTTEAS A T A1 AT CSOC Subject: RE: C-8 BIOFATE PROGRAM - WELL SAMPLING Dan, this needs to be discussed with Dave Ellis before we alter from our current program.

RECYCLED o> AR226-2674 PROJECT LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 LH DELINEATION 8/02 SRCNAME FBLK-2 FBLK1 FBLK1 FBLK1 FBLK1 FBLK1 FBLK1 FBLK2 FBLK2 FBLK2 LHPSD1 LHPSD2 LHPSD3 LHPSD5 LHPSDTW1 LHPSDTW10 LHPSDTW11 LHPSDTW12 LHPSDTW2 LHPSDTW3 LHPSDTW4 LHPSDTW5 LHPSDTW6 LHPSDTW6 LHPSDTW9 LHWAE1 LHWAFBLK LHWAFBLK1 LHWAFBLK3 LHWAFBLK4 LHWAN1 LHWANE1 LHWANE1 LHWANE2 LHWANW1 LHWANW1 C8 results Envista 111104.xls wwo THEME DATESMPL SMPLT SAMPLE_NUMBER Blank Water 8/23/2002 FB WWO-K-FBLK-2 Blank Water 8/20/2002 FB WWO-K-FBLK1 Blank Water 8/21/2002 FB WWO-K-FBLK1 Blank Water 8/22/2002 FB WWO-K-FBLK1 Blank Water 8/26/2002 FB WWO-K-FBLK1 Blank Water 8/28/2002 FB WWO-K-FBLK1 Blank Water 8/30/2002 FB WWO-K-FBLK1 Blank Water 8/21/2002 FB WWO-K-FBLK2 Blank Water 8/27/2002 FB WWO-K-FBLK2 Blank Water 8/29/2002 FB WWO-K-FBLK2 Drinking Water 8/21/2002 FS WWO-D-LHPSD1 Drinking Water 8/21/2002 FS WWO-D-LHPSD2 Drinking Water 8/21/2002 FS WWO-D-LHPSD3 Drinking Water 8/21/2002 FS WWO-D-LHPSD5 Groundwater 8/22/2002 FS WWO-G-LHPSD-TW1 Groundwater 8/22/2002 FS WWO-G-LHPSD-TW10 Groundwater 8/21/2002 FS WWO-G-LHPSDTW11 Groundwater 8/21/2002 FS WWO-G-LHPSDTW12 Groundwater 8/22/2002 FS WWO-G-LHPSD-TW2 Groundwater 8/22/2002 FS WWO-G-LHPSD-TW3 Groundwater 8/23/2002 FS WWO-G-LHPSD-TW4 Groundwater 8/23/2002 FS WWO-G-LHPSD-TW5 Groundwater 8/22/2002 DUP WWO-G-LHPSD-TW6-2 Groundwater 8/22/2002 FS WWO-G-LHPSD-TW6 Groundwater 8/22/2002 FS WWO-G-LHPSD-TW9 Groundwater 8/30/2002 FS WWO-G-LHWAE1 (21-26) Blank Water 8/23/2002 FB WWO-K-LHWAFBLK Blank Water 8/26/2002 FB WWO-K-LHWAFBLK1 Blank Water 8/28/2002 FB WWO-K-LHWAFBLK3 Blank Water 8/29/2002 FB WWO-K-LHWAFBLK4 Groundwater 8/29/2002 FS WWO-G-LHWAN1 (17-22) Groundwater 8/20/2002 FS WWO-G-LHWANE1 (21'-27') Groundwater 8/22/2002 FS WWO-G-LHWANE1 (56'-58') Groundwater 8/30/2002 FS WWO-G-LHWANE2 (20.7-25.7) Soil 8/29/2002 FS WWO-S-LHWANW1(0.1-0.5) Soil 8/29/2002 FS WWO-S-LHWANW1 (5.0-5.5) Page 1 AR226-2674 i TOP BOTTOM FILTERED CASNO ANALYTE RsItPost T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 <50 T 3825261 FC-143 3650 T 3825261 FC-143 4260 T 3825261 FC-143 952 T 3825261 FC-143 8090 T 3825261 FC-143 810 T 3825261 FC-143 1100 T 3825261 FC-143 1730 T 3825261 FC-143 824 T 3825261 FC-143 80.6 T 3825261 FC-143 4170 T 3825261 FC-143 12300 T 3825261 FC-143 6260 T 3825261 FC-143 1230 T 3825261 FC-143 1150 T 3825261 FC-143 812 21 26 T 3825261 FC-143 416 T 3825261 FC-143 <0.020 T 3825261 FC-143 <0.020 T 3825261 FC-143 <0.020 T 3825261 FC-143 <0.020 17 22 T 3825261 FC-143 50800 21 27 T 3825261 FC-143 5580 56 58 T 3825261 FC-143 662 20.7 25.7 T 3825261 FC-143 1280 0.1 0.5 T 3825261 FC-143 110 5 5.5 T 3825261 FC-143 6.1 RsItVal UNITS 25 NG/L 25 NG/L 25 NG/L 25 NG/L 25 NG/L 25 NG/L 25 NG/L 25 NG/L 25 NG/L 25 NG/L 3650 NG/L 4260 NG/L 952 NG/L 8090 NG/L 810 NG/L 1100 NG/L 1730 NG/L 824 NG/L 80.6 NG/L 4170 NG/L 12300 NG/L 6260 NG/L 1230 NG/L 1150 NG/L 812 NG/L 416 NG/L 0.01 ug/l 0.01 ug/l 0.01 ug/l 0.01 ug/l 50800 NG/L 5580 NG/L 662 NG/L 1280 NG/L 110 ug/kg 6.1 ug/kg Hit LAB_ANALYTE QUAL_EPA 0 FC-143 0 FC-143 0 FC-143 0 FC-143 0 FC-143 0 FC-143 0 FC-143 0 FC-143 0 FC-143 0 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 0 FC-143 0 FC-143 0 FC-143 0 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 1 FC-143 'aM iN s.'

LZ JOLI TRADE SECRET Study Title Hydrolytic Stability of H-25435 as a Function of pH D u P on t-14965 Test Guidelines: OECD Guideline 111 Author: Bogdan Szostek, Ph.D.

AR226-2785 FOR DU PONT USE ONLY Copies to: ,,.

% Material Tested; Material Submitted AR226-2829 Copies t0\ i Haskell L a b o rstoryyUtfoorrn,TrooxxiiccoolloogOyUrSartd inCdumsptar"iyal Medicine HASKELL LABORATORY REPORT NO. 244-71 "" rganic Chemicals Department 'Jackson Laboratory Haskell No.; 6917 Procedur ACUTE ORAL Test ~L "-s zstz&z --------- ------------- ------------- -- young adult r ' the livers were removed for gravimetric Company Sanitized.

AR226-3106 H n tin g d o n V CONFIDENTIAL DPT 437/984087 i ACTIVATED SLUDGE - RESPIRATION INHIBITION TEST Sponsor Dupont Speciality Chemicals, Jackson Laboratory, Chambers Works, Deepwater, NJ 08023, USA.

M .M & 414 TRADE SECRET Study Title H-24768: Developmental Toxicity Study in Rats Laboratory Project ID: DuPont-6515 DuPont-6515 T e s t G u i d e l i n e s : U.S.

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b -- --------------------------------------------- ------------------------------------------------------------------------------ 498 fa ite 3 3 DuPont-13882 TRADE SECRET Study Title H-26137, H-26138, and H-26139: Repeated Dose Dermal Toxicity 28-Day Study in Male Rats Test Guidelines: U.S, EPA Health Effects Test Guidelines OPPTS 870.3200 (1998) OECD Guideline for the Testing of Chemicals Section 4: Health Effects, Number 410(1981) Author: Carol Finlay, B.A.

AR226-3351 - Continuous flow-through - 14 volume renewals/dav - Negative, positive control (PFOS), 4 test substances - Single test concentration ~ 10 pg/L as total fluorine - nominal test substance cone's from 13 - 100 pg/L -1 test chamber/test substance; 70 fish /test chamber - 16 L : 8 D photoperiod - 14 d uptake phase, 14 day depuration phase - Fish fed - 2% body weight; frozen brine shrimp - Loading - 0.2 g/L/day .esu Stock Solutions - % Recovery of Test Substance as Fluoride a DayO @Day 13 esults PFOS Uptake and Depuration O Measured m Modeled Uptake 4 Modeled Depuration Fluoride, ug/g Day Results 8.000 Urethane Polymer Uptake and Depuration o Measured Modeled Uptake a Modeled Depuration 7.000 6.000 5.000 Fluoride, ug/g 4.000 3.000 2.000 1.000 0.000 o o $ oo 10 O O <0 ----- A A A A A Si 00 AA 15 20 Day Fluoride, ug/g Resulti Zonyl BA (Telomer B Alcohol) Uptake and Depuration O Measured Modeled Uptake 4 Modeled Depuration 14.000 12.000 10.000 8.000 XO>- 6.000 4.000 2.000 0.000 -c O -ooo o o 10 oo o o 15 Day OO 20 -ft25