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AR226-2785 FOR DU PONT USE ONLY Copies to: ,,. E I. d>! Port de Nemours and Cb., Inc Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P, 0. Box 50, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 788-82 Material Tested* " Octanoic acid, pentadecafluoro-, ammonium salt Haskell No. 12,037 Other Codes C-8 Study Initiated/Comnlet-ed *! 82-10/15/82 Material Submitted bv CR&D Department Haskell Laboratory ACUTE ORAL TOXICITY OF S A FUNCTION OF AGE S ^ o r , H s e s ^ o ^ U ,i ,,(orn Cta"0ate Sll8htl!r toxic when administered In 8-1U weeks jld)* ma'p<; insn ,7n ' ,, rL,: males, LD50 - m 580 mg'kS> young adults (approx. lD^ ' ld" m regards to the sex or It = 258 mg/kg` toxicity newborn (.< 2 days old) and old r 1_OW!l j "? S18nificant difference. However, young adult rats! ratS had iOWer U 5 v^lues tha" weanlings o^ b-'Tntragastric iniJb^ion ' " fusf nsins corn oil, was administered groups S e n ? S p v k S,lngle dSeS t0 grouPs of Crl:C0* rats. The old) U) L i V ;.Newb ^ 2 days old); (2) Female - Newborn 2 days old - S) ? ! ," Wef ilngs <2i days old); (4) Female - Weanlings (2 davs anj determine the initial dose ]p,, , 1 ^ < ^ L 'tSZ RanSe E n ding Study was conducted to rats**. The surviving rate u u"^ LD5 t6St n Newborn (< 2 days old) recovery period and then sacrificed^ T h e ^ O ^ a r ^ during ? U _ d a y the .relit, data usin6 tta m.th:d 'ofTS: H i TM " !" " C8lCUlated ft" 1TSCA CB1 Scn^-Doccnotc^ Company Results; Dose jrcg/fcg) Average Body Weight (g) Suspension ) Male - Newborn (< 2 days old) Average Dose (mL) 370 6 . 2 330 6 . 1 280 6.4 240 6 .2 200 6.9 130 7.6 1 . 0 0.23 1.0 0.20 1 . 0 0.18 1 .0 0.15 1 . 0 0.14 1.0 0.10 Deaths occurred up to 6 days after dosing. Mortality Ratio 10/10 10/10 10/10 A/ 10 00//1100 LD50 **** 243 ng/kg female - Newborn 2 days old) 320 5.8 2 au 5.7 240 6 .2 220 7.1 200 8 .0 160 6.0 130 7.7 1 . 0 0.19 1 .0 0.16 1 . 0 0.15 1 .0 0.15 1 .0 0.16 1.0 0.10 1.0 0.10 Deaths occurred up to 4 days after dosing. 10/10 6/10 5/10 0/10 0/10 0/10 0/10 258 mg/kg lale - Weanlings (21 days old) 710 67o 525 450 400 35o 68 82 69 87 84 87 3.0 O.5r 1.62 2.21 8/10 7/10 573 mg/kg 3.0 1 . 2 0 3/iO 2.5 0c 1.56 2/10 0c 1.34 1.21 2/10 0/10 Clinical Signs: Deaths occurred Stained and up to 3 days wet perineal after dosing area, weakness and weight loss. iemale - Weanlings (21 days old) 67u 80 450 87 400 80 350 82 2.5 2.14 6/10 D 1.55 3/10 1 .3 1.28 3/10 5 1.15 1/10 Clinical Signs; Stained and wet weight loss. Deaths occurred up perineal area, stained face to 3 days after dosing. 580 mg/kg weakness and iujeis 2SanUitcd. 0** Comesi Male - Young Adults (approx. 8-10 weeks *70 ag/kg Female - Young Adults (approx. 8-10 weeks old) 670 185 500 185 425 182 350 180 5.0 2.48 5.0 1.85 5.0 1.54 5.0 1.26 180//1100 2/10 1/10 453 mg/kg d isch a rg erd ia rrh fra n fwiight s s " ^ " ^ ^ area' nasal dosing. g s* occurred up to 4 days after Male - Older Rats 720 563 500 558 400 543 350 564 300 501 240 495 20 648 10 10 10 10 3 3 3 4.06 2.79 2.18 1.98 4.95 3.96 4.32 1100//1100 10/10 009///111000 0/10 336 mg/kg tremors, lefhargy^chro^^ occurred up to 9 days after dosing. ' lneal " * a ^akness, h d ght loss* Deaths Female - Older Rats 67u 350 450 355 < 00 365 350 358 225 346 10 10 10 10 3 2.34 1.60 1.46 1.26 2.55 10/10 9/10 6/10 5/10 1/10 343 mg/kg * Purity; Contaminants Synonyms:_^o Ammonjuun perfluorooctanoate Sanitized. Does 3 - CoWpan n i a * T SCAC81 ** f i n d i n g Study gesuita, Newborn Ra-. Dose Mortality i mg/kg Male Female 130 210 300 375 425 500 575 *** Finney, D. J Press. 0/4 1/11 0/7 0/3 2/5 5/6 4/5 7/7 3/3 7/7 1/1 8 /8 7/7 4/4 - Probit Analysis. 3rd Ed., 1971, Cambridge University **** 9^5% Confidence Limite Male - Newborn (< 2 days old) female -- Newborn (< 2 days old) lower (mg/ke) Undefined 244 hale - Weanlings ( 2 1 days old) Female - Weanlings ( 2 1 days old) Male - Young Adults (approx. 8 -1 0 weeks old) 514 470 403 female - Young Adults (approx. 8 -1 0 weeks old) 413 Male - Older Rats Undefined female - Older Rats ioc Upper (mg/kg) 276 Slope 55.7 19.9 664 2,258 536 8.0 4.7 8.6 503 15.0 390 8 .0 " i '- f Reviewed by: Study Director LH:tac:WP:li.a Approved by: ___ , i era^d L. Kennedy, J p . Section Supervisor' Acute Investigations Haskell Lab. Report No. 788-82 5 - noi contain TSCACBl Sanlfeed.Does