Katherine E.

Wildlife International, Ltd. -1 - Project Number 454A -106 PFOS: A 96-HOUR SHELL DEPOSITION TEST W ITH THE EASTERN OYSTER (Crassostrea virginica) ADDENDUM WILDLIFE INTERNATIONAL, LTD.

Group Summary P Generation Group Group 1 Group II Group III Group IV Group V NUMBER OF DAYS OF DATA COLLECTION NUMBER OF DAYS IN ESTRUS % OF DAYS IN ESTRUS 21 g 43% 21 8 41% 21 9 42% 21 8 39% 21 9 42% NUMBER OF DAYS IN % OF DAYS DIESTRUS IN DIESTRUS NUMBER OF % OF DAYS DAYS IN IN PROESTRUS PROESTRUS 9 41% 9 45% 9 45% 10 45% 9 43% 3 16% 3 14% 3 13% 3 15% 3 15% "N" FOR MEAN CYCLE MEAN CYCLE NUMBER OF LENGTH LENGTH CYCLES 30 4.3 4.6 30 4.0 4.6 28 4.1 4.3 30 4.3 4.4 29 4.1 4.6 P Generation nimal I 13801 13802 13803 13804 13805 13806 13807 13808 13809 13810 13811 13812 13813 13814 13815 13816 13817 13818 13819 13820 13821 13822 13823 13824 13825 13826 13827 13828 13829 13830 13831 13832 13833 13834 13835 13836 13837 13838 13839 13840 13841 13842 13843 13844 13845 13846 13847 13848 13849 13850 Group 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 MEAN CYCLE LENGTH 3.75 3.20 3.75 3.67 3.75 5.33 4.00 4.25 4.25 4.25 4.50 9.00 4.00 5.00 5.00 4.33 4.00 4.00 4.00 4.00 4.25 4.00 4.00 3.40 4.67 3.75 4.25 3.75 4.00 4.00 3.67 4.25 4.00 3.75 4.00 4.00 4.00 4.50 3.75 4.33 3.75 4.00 4.25 3.75 4.00 4.00 4.00 4.00 4.00 4.25 NUMBER OF CYCLES 5.00 6.00 5.00 4.00 5.00 3.00 5.00 4.00 5.00 5.00 4.00 2.00 5.00 4.00 4.00 5.00 5.00 5.00 5.00 4.00 5.00 5.00 5.00 6.00 5.00 5.00 5.00 4.00 4.00 5.00 5.00 5.00 5.00 5.00 5.00 2.00 5.00 2.00 5.00 5.00 4.00 4.00 5.00 4.00 4.00 5.00 4.00 5.00 5.00 6.00 13851 13852 13853 13854 13855 13856 13857 13858 13859 13860 13861 13862 13863 13864 13865 13866 13867 13868 13869 13870 13871 13872 13873 13874 13875 13876 13877 13878 13879 13880 13881 13882 13883 13884 13885 13886 13887 13888 13889 13890 13891 13892 13893 13894 13895 13896 13897 13898 13899 13900 13901 13902 CO CO CO P Generation 2 3.25 6.00 2 3.75 5.00 2 4.00 4.00 2 4.00 5.00 2 3.75 5.00 2 3.75 4.00 2 4.50 4.00 2 4.00 5.00 2 3.80 5.00 2 4.25 5.00 3 5.00 3.00 3 3.75 5.00 3 4.00 5.00 3 4.67 4.00 3 4.00 4.00 3 4.67 3.00 3 4.00 5.00 3 3.75 5.00 3 4.00 5.00 3 4.00 4.00 3 3.75 5.00 3 3.75 5.00 3 4.00 5.00 3 5.00 3.00 3 4.00 4.00 3 3.75 4.00 3 4.25 4.00 3 4.25 5.00 3 4.00 6.00 3 4.00 4.00 3 5.00 4.00 3 4.00 5.00 3 3.75 5.00 4.00 5.00 #DIV/0!

Estrous Cycling and Natural Delivery/Litter Observation Data FI Estrous Cycling Data, From Data Analysis Presented at EPA on August 30,2002" APFO Dose Group (mg/kg/day) 0 1 3 10 30 All 4-Day 14/30 19/29 9/30 15/29 15/30 Cycles 3-5 Day Cycles 24/30 26/29 21/30 25/29 27/30 Cycle Length (Days) 2 3 4 5 6 7 8 Mean Cycle Length (Days) S.D. 1 2 75 18 5 1 2 4.3 0.8 Incidence of Cycle Length Per Group 0 1 80 16 2 1 0 4.2 0.5 5 7 57 32 5 0 1 4.3 0.9 2 5 84 14 3 0 0 4.1 0.6 5 5 86 17 0 0 2 4.1 0.8 Aberrant Cycles Parental > 4 Days Diest. 2 1 14 1 > 3 Days Est. 17 13 15 16 13 FI > 4 Days Diest. 5 3 3 5 3 > 3 Days Est. 25 25 29 11 14 P E M D End in Estrous Distribution of Cycle Status at Start of 21-Day Evaluation Period, FI 14 12 8 10 11 14 6220 15 13 16 13 Distribution Rats in Estrous at End of 21-Day Evaluation Period, FI 13 17 18 19 5 11 2 12 19 Mean Implantations, Liveborn, and Stillborn S.D. per Litter by Group c Parental Litters Implantations 15.3 1.9 15.6 2.1 15.2 2.3 15.6 2.0 15.3 2.0 Liveborn 14.2 2.2 14.2 2.6 13.4 3.6 13.8 3.0 13.4 3.3 Stillborn 0.1 0.3 0.4 1.4 0.2 0.4 0.1 0.4 0.2 0.6 F I Litters Implantations 14.8 3.0 15.8 2.1 14.7 2.8 15.0 2.9 14.5 2.9 Liveborn 13.6 2.9 13.7 2.4 13.8 3.0 13.5 3.1 13.3 2.8 Stillborn 0.2 0.5 0.2 0.5 0.2 0.6 0.2 0.5 0.1 0.2 "Original analysis counted cycles from the day following estrous (or proestrous if diestrus followed proestrous without observation of estrous) to the day following the end of the next estrous.

AR0U'OM3 C O R N IN G Hazleton MUTAGENICITY TEST WITH // ^ '< A \ T-6342 i \* IN THE SALMONELLA - ESCHERICHIA COI//MAMMALIAN-MICRSOME REVERSE MUTATION ASSAY ir-- - ^ FINAL REPORT AUTHOR Timothy E.

/ H U U -05 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE________________________________________________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, FC-143 or as the major component of FC1015.

3M Dr.

f l& 2 6 -1 6 f t FFOS: A PILOT REPRODUCTION STUDY WITH THE NORTHERN BOBWHITE FINAL REPORT WILDLIFE INTERNATIONAL, LTD.

TherImmune R esearch C orporation smosso. 3M Corporate Toxicology Building 220-2E-02, 3M Center St.

EXHIBIT 1 Table A.

NORTHERN KENTUCKY OFFICE SUITE 340 1717 DIXIE HIGHWAY COVINGTON, KENTUCKY 41011-4704 SOS-331-2S3S 513-3S1-2S3S FAX: 513-381-6613 DAYTON, OHIO OFFICE SUITE 900 110 NORTH MAIN STREET DAYTON, OHIO 45402-1786 937-22B-2S3S FAX: 937-228-2616 Robert A, Bilott (513) 357-9638 bilott@taftlaw.com TAFT, STETTINIUS & HOLLISTER LLP 425 WALNUT STREET, SUITE 1800 CINCINNATI, OHIO 45202-3957 513-381 -2838 FAX: 513-381-0205 www. taftlaw. com CLEVELAND, OHIO OFFICE 3500 BP TOWER 200 PUBLIC SQUARE CLEVELAND, OHIO 44114-2302 216-241-2838 FAX: 216-241-3707 COLUMBUS, OHIO OFFICE 21 EAST STATE STREET COLUMBUS, OHIO 43215-4221 614-221-2638 FAX:614-221-2007 /9S May 12,2005 FEDERAL EXPRESS Dr.

AR226-2101 Test Results .

TestResijilts AR226-2123 File:///C|/WINDuws/1emp/U-B-LL

i AR226-2150 Roger J .

AR226-2203 Robert F Pinchot 10/18/2001 02:30 PM To: ck Subject jh ans haw @ m ail.d ep.state.w v .us ' Debbie J Mulrooney/AE/DuPont@DuPont, Steven L Grise/AE/DuPont@DuPont, Bernard J Reilly/AE/DuPont@DuPont, M.