P)E Be ANOTRRRTEARR an EP1757581A1 12) EUROPEAN PATENT APPLICATION (43) 2D8a.t0e2o.f20pu0b7licaBtiuonl: t 200709 (51)SIontrClt. aomtarmnen core somone 1) Aopteston umber os2s4362.2 22) Oat of ing: 18.08.2008 84) ADeTsGigEnaBtGedCCHonCYracGiZnDgESaDtKe EE ES FIFRGB GR| 72)+Smyewnetto,rsRobert George SHKU IE IS IT LILT LU LV MC NL PL PT RO SE SI + AClalmepnitoonw,n,BPetAh1A8m10n3-5451 (US) ADeLsGigAnaNtRedKYU Extension States: 90) priory: 24082005 Us 211388 (71) lAgepnitcoawsn:,iPAPr1o9d1u9c5t-1s5a0n1d(CUSh)emica, inc

3M Medical Department Study: T-6889.3 3M Medical Department Study: T-6889.3 Analytical Report: FACT-TOX-026 LRN-U2782 Analytical Report: FACT TOX-026 LRN-U2782 Study Title 26-Week Capsule Toxicity Study with Ammonium Perfluorooctanoate (APFO/POAA) in Cynomolgus Monkeys Amended Analytical Laboratory Report Title Determination of the Presence and Concentration of Perfluorooctanoate Fluorochemical in Liver, Serum, Urine and Feces Samples Data Requirement Not Applicable Author 3M Environmental Laboratory Analytical Phase Completion Date Juant seig1n1in, g2001 Performing Laboratories Liver, Serum and Urine Analyses Feces Analyses 3M Environmental Laboratory Building2-3E-09, 935 BushAvenue St.

Sponsor: 3M St.

"b5 Xenochemical Receptor trans-Activation by Perfluorooctane-based Chemicals "r-_ _5-._- _j,__ "7"--(-o_XO -_5 / Co_, _ J -1_-_. 3 "n,\-b_5.

STUDY TITLE Extraction of Potassium Perfluorooctanesulfonatefrom Egg Membrane, Albumen, and Yolk for Analysis Using HPLC-ElectrosprayMass Spectrometry DATA REOUIREMENTS Analytical Method Requirements STUDY DIRECTOR Sean Gallagher STUDY COMPLETED ON September 06,2001 /- PERFORMING LABORATORY / TESTING FACILITY Centre Analytical Laboratories, Inc.

3M General Offices ES38t1M7PCaeln,te1Mr1N10$514.10 July 14,2004 BM ve voi UESPAEnEvaistr,onRmoeontmal44P1r0otection Agency pr W1a2s01hiCnogntsotni,tuDtiCon A2v0e4n6u0e, NW VIA UPS Re: Information on Perfluorooctane Sulfonates and Related Compounds, CD No. 6 Dear Mary: cThoimspocuonntdisn,ueass 3paMr'tosfvooluurntoanrgyoisnubgmdiisaslioognuseowfitdhatEa PoAn preergfalrudoirnogocftluaonreoscuhlefmoincaatless. anWderealraeted enclosing compact disc (CD No. 6) containinag numofbstuediers, as more fully described.

AR226-2272 xcr""" ^f :-- 9' Dabbie J Mulrooney 04/10/2002 10:22 AM To: cc: Subject: Catherine A Barton/AE/DuPont@DuPont Wash Works C8 Max Concentration Cathie, You asked me the other day for the maximum concentration value for the Washington Works C8 modeling.

DuPont Fluoroproducts AR226-2342 DuPont Fluoroproducts Chestnut Run Plaza P.O.

AR226-3392 Subchronic Toxicity 90-Day Oral Gavage Study of of 8-2 Telomer B Alcohol in Rats G.S.

oe{R~w 44 " 129 202( N) 1706- _ /ORI Gl NAL PAPER .

3M EnvironmentaLlaboratory FinalReport-AnalyticSatludy Single-DoIsnetravenouPsharmacokinetSitcudyofT-6054inRabbits In-VivSotudyReferencNeumber:HWI#6329-138 StudyNumber: ANMT-122094.2 TestSubstanceF:C-129(T-6054) Name and AddressofSponsor: 3M SCD Division 367 Grove Street St.Paul,MN 55106 Name and Address ofTestingFacility: 3M EnvironmentalTechnology& Services 935 Bush Avenue St.Paul,NlN 55106 Method Numbers AMDT-M-1-0, AMDT-M-2-0, AMDT-M4-0, AMDT-M-5-0, AMDT-M-8-0, and Revisions: Thermal ExtractionofFluorideby Means of a Modified Dohrinann DX2000 OrganicHalideAnalyzer-Liver FluorideMeasurement by Means ofan Orion EA940 Expandable Ion Analyzer ExtractionofFluorochemicalsfrom RabbitLiver Analysisof RabbitLiverExtractforFluorochemicalsUsing ElectrospraMyass Spectrometry Analysisof FluorideUsing the SkalarSegmented Flow Analyzer with Ion SelectivEelectrode InitiatioDnate: See attachedprotocol Author:James D.

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (COROPHIUM VOLUTATOR) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonicacid)(CAS # 2795-39-3).

am Lest 7/ 2(/ AR 226 _ 350 SHENVIRONM`ENPTARL LONASOJOERE0A5TC0ORT2Y Analytical Report Coma. ~ Cay Definitive Study: Quantitative Screening of Commercial Lots of Human Serum Obtained in 2004 for Endogenous Fluorochemicals Using Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry LaboratoryRequest Number: 05-0442 Testing Laboratory 3M Environmental Health and Safety Operations 3M Environmental Laboratory - eee 8 3M Environmental Laboratory 5 UR MaPrrkojEe.ctEfLleeafdson BPuiOldBinogx23-333E3-109 Saint Paul, MN 55133-3331 Phone: 651-778-5405 2 z 3 g=z m= s Te`sWtiilnigamFaKc.ilRiteyaMgaenn,agPehm.De.nt BPuiOldBinogx23-333E3-109 Saint Paul, MN 55133-3331 Phone: 651-778-6565 Requestor BuiDladliengB2a-co3n-0 PO Box 33331 SaintPaul, MN 55133-3331 Phone: 651-778-4736 Page tof1o ----------------ee-- e ---- ee-- e SEWNVIRONMEPNRTAOLILNEAOBCOER0TA8T0O4R2Y 3M Environmental Laboratory Analytical Report Project Number: E05-0442 1 Introduction / Summary `The following analytical report presents data from commercially available normal `pooled human serum that were purchased by the 3M Environmental Laboratory and. subsequently screened for endogenous fluorochemicals.

3M 3M General Offices August 1, 2008 3M Center p ~ St.

3\1 General Offices 3M Center St.