Perfluoroalkylated substances Aquatic environmental assessment c=> O^o 5 ~ ^rn 03 T3D -tm "X -*C p* (.

AKAS6- 267 FlUO RIIW ED SDREAC1ANTS AND REPELLENTS RovSiseocdonadndEEdxitpioannded Erik Kissa W ilmiCngotnosnu, lDtaenltaware MARCEL OEKKER Marcel Dekker, In c.

f fi# - 3-T7 SRPT T-76UI MIN 315/022356 PERFLUOROOCTANESULFONYL FLUORIDE (POSF) T ~lbbl3> IN VITRO MAMMALIAN CHROMOSOME ABERRATION TEST IN HUMAN LYMPHOCYTES Sponsor 3M Center 3M Corporate Toxicology Building 220-2E-02 St Paul MN 55133-3220 USA Page 1 of 31 Research Laboratory Huntingdon Life Sciences Ltd Woolley Road Alconbury Huntingdon Cambridgeshire PE284HS ENGLAND Report issued 22 October 2002 001542 MIN 315/022356 CONTENTS Page CONTENTS................................................................................................................................ 2 COMPLIANCE WITH GOOD LABORATORY PRACTICE STANDARDS..................... 4 QUALITY ASSURANCE STATEMENT................................................................................... 5 RESPONSIBLE PERSONNEL................................................................................................... 6 SUMMARY................................................................................................................................ 7 INTRODUCTION.................................................................................................................. 8 TEST SUBSTANCE.............................................................................................................. 10 EXPERIMENTAL PROCEDURE......................................................................................... 11 ASSESSMENT OF RESULTS................................................................................................... 15 MAINTENANCE OF RECORDS.............................................................................................. 15 RESULTS.............................................................................................................................. 16 CONCLUSION........................................................................................................................... 17 REFERENCES............................................................................................................................ 18 :2 : 001543 MIN 315/022356 COMPLIANCE WITH GOOD LABORATORY PRACTICE STANDARDS The study described in this report was conducted in compliance with the following Good Laboratory Practice standards, with the exceptions stated below, and I consider the data generated to be valid.

3M GLP10-01-02; Interim Report 06 Analysis of PFBS, PFHS, and PFOS In Water Samples from Decatur, AL June/July 2010 GLP10-01-02: Interim Report 06 - Analysis o f PFBS.

Analytical Report Fluorochemical Characterization of Aqueous Samples Decatur Outfall 001 FC Monitoring (E03-0848) Exygen Research Laboratory Report No.

INTERIM REPORT #28 - Analysis of Groundwater Samples STUDY TITLE Analysis of Perfluorobutanesulfonate (PFBS), Perfluorohexanesulfonate (PFHS), and Perfluorooctanesulfonate (PFOS) in Water, Soil, Sediment, Fish, Clams, Vegetation, Small Mammal Liver and Small Mammal Serum Using LC/MS/MS for the 3M Decatur Monitoring Program DATA REQUIREMENTS EPA TSCA Good Laboratory Practice Standards 40 CFR 792 STUDY DIRECTOR Jaisimha Kesari P.E., DEE Weston Solutions, Inc. 1400 Weston Way West Chester, PA 19380 Phone: 610-701-3761 INTERIM REPORT COMPLETION DATE January 23, 2007 PERFORMING LABORATORY Exygen Research 3058 Research Drive State College, PA 16801 Phone: 814-272-1039 STUDY SPONSOR 3M Company 3M Building 0236-01-B-10 St.

3M Environmental Laboratory IU26-002S M ethod E xtraction of Potassium Perfluorooctanesulfonate or other FLUOROCHEMICAL COMPOUNDS FROM URINE FOR ANALYSIS USING H P L C - Electrospray/M ass Spectrometry/M ass Spectrometry Method Number: ETS-8-96.0 Adoption Date: Author: Lisa Clemen, Glenn Langenburg Revision Date: /\/ j\ Approved By: Laboratory Manager v s f i f i n ------ Group Leader n l fr cluvu!

3M Environmental Laboratory AlWo e-os-ftyo M ethod Determination of Perfluorooctane sulfonate (PFOS), Perfluorooctane SULFONYLAMIDE (PFOSA), AND PERFLUOROOCTANOATE (POAA) IN W ATER BY LlQUIDSolid Extraction and High-Performance Liquid Chromatography/Tandem Mass Spectrometry (HPLC/MS/MS) Method Number: ETS-8-154.0 Adoption Date: Author: Kristen J.

Larry R.

BACK TO MAIN AR226-1030A012 Study Title Solubility Study of Perfluorooctanesulfonate (PFOS), SD-018 and Perfluorooctanesulfonamide (FOSA), SE-027 in Male/Female Human Urine Data Requirement 40 CFR 160.105(b) Author Mark E.

AR226-1030A020B BACK TO MAIN 3M Company Page 1 o f 36 FINAL REPORT Epidemiology, 220-3W-05 Medical Department 3M Company St.

BACK TO MAIN BIODEGRADATION (Aerobic Soil and Sediment Cultures) TEST SUBSTANCE___________________________ Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 217 (TN-A-2130).

CHRONIC TOXICITY TO FRESHWATER INVERTABRATES (Daphnia magna) TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder.

(s s ) c IN VITRO MICROBIOLOGICAL MUTAGENICITY ASSAYS OF 3M COMPANY COMPOUNDS T-2540 CoC and T-2541 CoC Final Report August 1979 By: Kristien E.

A R W -o H IJ ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: [2-(N-Ethylperfluorooctanesulfonamido) ethyl acrylate; may also be referred to as B1228, D-1, EtFOSEA, or FX-13. (2-Propenoic acid, 2[ethyl[heptadecafluorooctyl)sulfonyl]amino]ethyl ester, CAS # 423-82- 5) Remarks: Material is an amber solid.