Document 0gamG1vdZDRXXrb964M39QgGd
AR226-3048
study Title Approximate Lethal Dose (ALD) of H-21951 in Rats
I ^^nratory ProjgeB Haskell Laboratory Report No. 796-96
Author Tracy A. Filliben
Study Completed On August 30, 1996
Performing Laboratory E I du Pont de Nemours and Company . . . , ' TnxicoloBV and Industriai Medicine Haskell LaboratoErlyktfoonr ^Rooxa,Cd,OP`.g^O- BBox 5ou0
]
1 Company Sanitized. Does not contain TSCA CBI
SubslanceTested: Synonyns/Codes:
n..Pont HLR 796-96
physical Fonti' Primposition:
rrmtaminants: Purity: Cnhmitter s NotgbaaL&jQ;: C ^R eaistQ L ie,'Sponsor:
Study lnitiat<*ri - Completed: In Life Phase Initiated - Completed.
DuPont Specialty Chemicals E. I. du Pont de Nemours and Company Wilmington, Delaware 6/21/96-8/30/96
6/22/96 - 7/8/96
Company Sanitized. Does not contain TSCA CBt
Approximate..
n.wmALP.." H J l g S L
Gsoffl! Ini'ojmianlCoiltlDlKJj
n,.Pnni HLR 79ft-%
Ail raw data and the final repo will ^ ^ " J ^ N e T o u r f a n d 1Company. Newark, Wilminaton, Delaware).
Company Sanitized. Does not contain TSCA CBI
SUMMARY
DuPont HLR 796-96
H-2.95. w a s a d m i n i s t e r e d a s ^ n o
deaths occurred during the stu y in
greater than 11,000 ing/kg
5000mg/kg) when administered as a si g
Company Sanitized. Does not contain TSCA CBI
oxitnaie Lethal Dose (A LP) of H-219S1 in Rals^
A T U R E PAGE
D uPont HLR 796-96
^uuuiQReport by:
r jd J b J k * ^ Tracy A. RlUben
Toxicology Associate
^ 7Worte by
Tracy A. RIHben Toxicology Associate
Reviewed and Approved for Issue:
Tracy A. Rlliben Study Director
Z 30%
TF/alw
Company Sanitized. Does not contain TSCA CB1
iM TPnniiCTlO N
The purpose of this , W`
w^de f i ^d as the lowest dose
1 ^ s ^ e i t h e r on the d * ofdosingor wHhin 14days post
exposure.
Ma t f p ia l s AND METHODS
A. A"f"H Husbandry Male CH:CD(SD,BR rats.
^ - Z ^ 'r .lZ t h e M 3 Rodent Chow. #5002 and water were available ad h b ttm .
T a^ C* * "
Haskell Ubomuny h *
-
monitored and administered by the ^a^ora1^ . f yie presence of coliforms, lead,
and other contaminants. Additional y, san^j
itolion by the cagewashers. Data
racks are periodically
AM M a
from this program m ^ ' ' " " ^ ^ T i ^ r a . t r i t k L l lequiremetlts and to be free
certified by the manufacturer t
pec ^
analyses, there is no evidence
~ P-iAndreiM,- -- 1 haw interfered with the results of this study.
'**" " " "
Rats were quaranuwd, weighed. one week. Animal rooms were mtunMnod TM
^
for ,,
h0 r
X T S retativc humidity o f 50% 10%. Excursions outside
S ^ w e r e of smaU magnitude and/or brief rhtr-irm and did no, adveme.,
affect the validity of the study.
B. Protocol
The test substance was administered nealto l j - a t g r d o s e ^ ^ y ^
Df visjbte
intubation. The density of th e test *"
wa^ 1 B n S S e stable under the
Dose rates administered ranged from 2300 to 11,000
t n ^ Lethal P *(A > m o fH -2 t9 5 l i n t o j ^
DuPont HLR 796-96
Jmg/kg of body wei.gh. t in in^cr.enmwennttssooff aauppDriooxxiimmaatieely 50%. Additional,l4y,w1arsat^was
dosed at 670 mg/kg. The dosing dqf was
were observed for clinical
day 15. F o llo w in g ^ m im s ^ o n o ^ te r t
^ i g ,* of toxicity
s^ s-IrisS rr:asm
performed.
RESULTS
The regimen a,,nka1mthe mworuailmityj U tag - - ' " 11W '" " P" *" 1 " *
detaitedtelow. No deaths occurred during the study.
Dosage (mg/kg)
670 2300 3400 5000 7500 11,000
Dose Volume ImL) 0.18 0.63 0.84 1.2 1.7 2.6
Density fmg/mU
Initial Body Weight (e)
271 284 253 244 237 247
Mortality No No No No No No
Company Sanitized. Does not contain TSCA CB1 -7-
B. rjjpical Siens
"JE L - TeS i - * --afte " b f c * f ^7500 mg/kg 1 day after do*.
at 670.2300. or 3400 mg/kg-
rpNCLUSION Under die condemns o f this study. die
(ALD grader
no! contain TSChC' smn*ed-D0s CotnPany
- H-
