THE EFFECTS OF CONTINUOUS AQUEOUS EXPOSURE TO 14C-78.02 ON HATCHABILITY OF EGGS AND GROWTH AND SURVIVAL OF FRY OF FATHEAD MINNOW (Pimephales promelas) .

ARllt-03G6 Attached are comments on the 3M Technical Report "Analytical Methodology and Support.

M S.a.6 - 0& 7.

0 //5A R M - I BIOACCUMULATION TEST SUBSTANCE Identity: [2-(N-Ethylperfluorooctanesulfonamido) ethyl acrylate: may also be referred to as B1228, D-1, EtFOSEA, or FX-13. (2-Propenoic acid, 2[ethyl[heptadecafluorooctyl)sulfonyl]amino]ethyl ester, CAS # 423-825) Remarks: Material is an amber solid.

flRUG'OHSO PHOTODEGRADATION TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, or FC-143.

fm-oi% BIODEGRADATION TEST SUBSTANCE Identity: Perfluorooctanesulfonate, Ammonium salt; may also be referred to as PFOS NH4+salt or FC-93. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, ammonium salt, CAS #29081-56-9) Remarks: The test sample is a mixture of the test substance in isopropanol and water (25% test substance, 20% isopropanol, 55% water).

m 'n4-oH9 qiZflia|mYmiiiv ) HI KER L A B O R A T O R I E S .

A R ^ r O 40| ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, or FC-109-X.

I A6 _ 0736 BIODEGRADATION (BOD/COD) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203A. (1Octanesulfonic acid) (CAS # 2795-39-3).

AR x a 6 - O g J-6 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) JE ST.SUBSTANCE_________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted.

BACK TO MAIN BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity: Potassium Decafluoro(pentafluoroethyl)cyclohexanesulfonate may also be referred to as FC-98.

A im & -0 0 0 7 2 14 Absorption of FC-95- C xn Rats After a Single Oral Dose October 26, 1979 Conducted at: During: Conducted by: Report by: Reviewed by: Riker Laboratories, Inc.

ARAZ6 _ 07& 3 ACUTE TOXICITY TO FISH (MUMMICHOG) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3).

AR 6 -0 7 3 !

ARZIG-ooSI Ecotoxicity Elements T itle ; 2- PFO S: A 96-H our Static A cute Toxicity T est with the Fathead Minnow (P im e ph a le s p ro m e la s ) PFO S: A 96-H o ur Toxicity T est with the Freshw ater Alga (S elenastrum capricom utum ) P FO S : A 48 -H o u r Static A cute Toxicity T est with the Cladoceran (.D a p h n ia m a g n a ) Laboratory or A uthor Wildlife International, Ltd.