Document MMa4q6Lb10gxb7D1KD5eMd5QV

ARJ%-O5oo ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro, ammonium salt, CAS # 3825-26-1) Remarks: The 3M production lot number was 83. The test sample is FC143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 -1 00% test substance and 0 - 3.5% C6, C 7, and C 9 perfluoro analogue compounds. METHOD: Method: Not noted. Type: Static acute GLP: No Year completed: 1978 Species: Bluegill sunfish (Lepomis machrochirus) Supplier: Not noted. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Not noted Test fish age: Not noted. Length and weight: Average length = 3.0 cm Average weight = 1.2 g Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry: Temperature: 19C Dissolved Oxygen: 9.9 ppm pH: 7.6 Stock and test solution preparation: Not noted Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Tanks containing 16 liters test solution. Number of replicates: one Number offish per replicate: 20 Loading rate: 1.5 g/L Number of concentrations: five plus a blank control Water chemistry during the study: Dissolved oxygen range (24 - 96 hours): 5.6 - 6.5 mg/L (control exposure) 4.9 - 5.9 mg/L (750 mg/L exposure)* pH range (24 - 96 hours): 003744 7.8 (control exposure) 7.6 - 7.8 (750 mg/L exposure)* * 750 mg/L test group (third highest concentration) data given because total mortality occurred in the highest test concentrations within 48 hours. Test temperature (24 - 96 hours): 19-20C RESULTS Nominal concentrations: Bk control, 420, 560, 750,1000, and 1350 mg/L Element value: 96-hour LC50 = 569 (500 - 636) mg/L Element value based on nominal concentrations CONCLUSIONS The test sample 96-hour LC50 for bluegill sunfish was determined to be 569 mg/L with a 95% confidence interval of 500 to 636 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY ______ Reliability: Klimisch ranking 3. Testing lacks information on the method followed. No information on stock or test solution preparations. Sample purity was not properly characterized and it lacks analytical confirmation of test substance concentrations. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 3844. OTHER Last changed: 5/24/00 003745 '>,S'4..** .<;:*' ENVIRONMENTAL ENGINEERING LABORATOKT AQUATIC TOXICItY DATA SHEET 'L i i M - ' -N ;TV-Tot < X k ~ U a w C Material Tested f C j ^O- Test Organism Test Solution Volume ^ m n ^ sNa. Avg. Wt. U 2 grams Avg. Size 3 C> cm \fe Liters/Tank No. Organisms per dose *2.O T im e Started 7 * . O t) p*Y\*. Date Started - 2 3 -7S?Date Completed S ~- 2 7 - 7 ? Dilution Water Carton Filter - Well Water (Temp. /9C.. P.O. ?,9 ppm, pH l.b V Investigator M .T :;/v LC 50 (95% Confidence Limits) SL ^ A Hp* . u roe Cone. C entro No. )rganism s Exposed <z o C\ 2 0 'Z .O 24 H rs. D .O . N o.D ead pH ppm O ns 6 ,r b IX 6S 9 S '0 7; OC i 2- ~2. CD r 2- \ i r 112 0 1 IX r ? OX 1.1 a 7 .7 zn T ._ . K l l C 48 H rs, D.O. N o. D ead pH ppm \ 7. g 6A "2 I X 6 . 0 2l 77 C6 1 l.b r n 2 0 7.1 C 2 0 i n r.c> o_ 2oC 72 H rs. D.O. N o.D ead pH ppm l 7 ,2 .0 7 ,7 r , 9 V 7 .6 t.b II 7 .7 2. 2.0 O IK 96 H rs. D .O . N o.D ead pH ppm t 7& 4 IS 1 o 7.7 3 4\ 7 . 7 4 . 9 2 .0 * 2 jO I9 c . Comments : - - - - - - - - - - - - - - - - - - - - - - BESJXQgY-AVAILABLF j --------------------------------------------------------------------------------------- ------ 003746