Document zznXXb0XrpX3J3xnNZxg7byjn
REPORT TO MONSANTO COMPANY THREE-GENERATION REPRODUCTION STUDY WITH
AROCLOR 1254 IN ALUINO RATS NOVEMBER ], 1971
IBT NO. B7297 I. Int rortuct ion
At the request of Monsanto Company, a reproduction study was initiated in order to ascertain potential toxicological effects of subacute oral administration of Aroclor 1254, Lot No. AK-IH, upon the fertility and offspring of three consecutive generations of albino rats.
This report presents the data collected from the initiation of the investigation to the weaning of the second (Fib) litters obtained from the third (F2) parental generation.
WATER PCB-SD0000034167
9f WATER PCB-SD0000034168
f
ApptOX. Hi. 1 Cl1 product U Kaacchlar
400
Ooaeclilor ^'
TAiLE 2~clUued
fCla: TibttUUo> of Llwer Moda let Ie^eru4 U >>U
res
Doe tag ta OUt Ha. Adraoaataua Nodular
Strata U* Darattaa (P) Aaiaala
Nod*lea
Hyperplaaia
Doaryu N 21-42 aha 30.5414
P
5 10
5 10
0 0 '0-
4-
Heaplaattc Nodulca
A4caooa
Repetoaii
Nr|>U* cellalar Carclaoaa
Cfc~
laugloNepaloua
Reference
___
- --- --- -"-
_ Kiatora 1 - (190)
-
-
Wiatar It 20-52 aka
It
.
0
- . - - - llo( el a
too 14
-
2
--
* - (1974)
500
0
-
0
- ---
-
ISOS
10
"
3
* " ~< -
~
40-42
Aroclor
Cltarlea N. 24 aoa. 0 23
1
0_
0 Levinafcaa
1242
River
F
| 32
-
1
- - 0 - 0 (1901)
to 29
-
1
- -0- 0
too 19
1"
3-
1
Kmrlilor
Uiiiar H 20-52 aka
0
It
.
0
--
llo. ri al
100 22
-
1
- - - - - (1974)
500 19
-
0
- '-
-
-
1000
15
*
0
- *-- -
2,2",5,5'-tera- -
- 24 aoa.
too
-
-
-
- ---
a. b 1 o rob1 |>bcy 1 *
Writ*** fc Nottiaili (1
* Fioa trlnkaan and deKok (1900).
. * AUtmt. Ilciiili liaiieil. 4 feevrme rootled. * tlluuol tolulioA in drlutlug wUf.
ADM 0 0 5 ^ 0 6
WATER PCB-SD0000034169
froduct
HoAa
Tin.
Other Trotseal1
T*U } SldlAM or CO-CABCIWOCEHESIj STUDIES WITB rci.
HoAc
Specie.
OhAervatlaaa
tanechlor 400
Diet
With
Kancrblor 300
Diet
With
Kmedilor 300 ^ Diet
With
tanechlor 400
Diet
With
K.acrhlor S00
Old
With
liMchUr
Cv|t
After
Arcelor 12)4
Oicl
Alter
Kincclilor 500*
Cv|e
Before
Arotlur 12)4
Old
With
tanechlor )00
Old . With
tanetlilor )00
Oict
Willi
Kanrthior 500
Diet
Before
tanetlilor 500
Old
Alter
20-HcCh a-MC 0-BNC
Ulerlae lap laat Diet
Diet
a-tOC
Old
OEN Water OUl Water
OEN Water
DEN*
Water
AFB,
Diet
2-FAA
Diet
J'-Me-DAB Diet
3 *-Me-DAB Diet
V-Mc-UAB Diet
44 amiac
Hepatocellular carclaoaa with 20-tleCb. None wltb coablaatloa.
44 aowae
Caablaallaa iacre.ted hepatocellular carclaoaa over a-BlIC aluae.
44 aouae
Coablaatloa produced bepetoceltutor carclaoaa. Each alone negative.
44 aouae
Coablaatloa laercased hepatocellular carclaoaa over a-BJIC alone.
Spr.gueDawley rat
Hepatocellular carclaoaa with DEN. None with coablaatloa.
Ulster ret
Coabination Increased hepatocellutar carclaoaa over OEN alone.
Spr.gucU.wlcy rat
Coabiaalloa Increased hepatocellular carcluoaa over DEN alooe.
Ulster rst
Coablnation decreased hepatocellular carcluoaa la oflsprlng.
Ita inbow trout
Nepalocellular carclaoaa with AKB|. None with coablaatloa.
SpragueDavlep rst
Nepat.cellul.r c.rciuuaa with 2-FAA. None with coal.la.tion.
Sprague-
Hepatocel lular carcluoaa with I'-tfc-DAB.
Daw ley rat None with coablaatloa.
SprsgueDivley rst
Hep.locellul.r c.rclnoa. with )'-Hc-0AB. Neae wltb ceabla.lloa.
Sprague* Dawley rat
Conblaotloo Increased hepatocellular carcloani over 3*-He-DAB olone.
I'
Bel.
UchlyMM, and Chib., 19ft
Ilo. et ol. 1373a ,b Nagasaki, el_ a 1. 1374, 1375 Ilo, et ol. 1373a,b Nagasah 4 ~"el_a|L 1974, 1375 N.g...hi, d_.r 1914, 19/S
Hahiuro, el_al. 1374
Ni.hixuai, 19/6, 19/9., b
Preston, el i|. 19A1
Ni.hizual, 19*0
Headrick., ei .1. 19//
H.kiur., cl .1. 19/4
H.kiur., rt .1. 19/4 Kiaura, ei'.K 1916 Kiuur., ct .1. 19/4
liniir, cl *1 . 19/6
AOM 0 0 5 9 6 7
WATER PCB-SD0000034170
Product
H#4
Tiua
Other Treatseat1
TAIUJ-CMtiiwi
HWWH or co-cauciiioceiiesis studies with rc
Made
Speclea
Ohaarvatiaaa
Did
Willi
leoechlor S00
Did
With
Kmiblor }O0 / C|t
iMtrliloi SOO
Ceva|e
tinetli lot S00
Savage
Uneyeiifiej Kmthlor
Did
After t With
After & With
After A With
After A With
Aroclor 125* Aroclor 125*
Shia Shia
After ieforc
V-lta-DAh a DEM
Did
S|kf*(ue* llarlcy ret
2-FAA * OU Dial
SpragueDawley ral
DEM
Water
Wlrur
DOT
Caia|t
ret
DEN
Water
Ulster
spd
Water
ret
DEM
Water
Ureter
srh, DOT Water, Csvega ret
2-FAA
Diet
Partial
kepaiecieay
Fischer ret
2,12-IMlA Skia
CDI aouse
TPA Skia COI SMuae
CoetlMllM decreoeeJ heyolocellolor rardsm ever J'-He-DAI DtN.
CaaLiaallaa decreased hepatocellular carclaaaa aver 2-FAA * DEM.
Caablaatioa iarreased hepatocellular carclaaaa aver DEM * MIT.
Cahblaatlea Iarreased hepatocellular carclaaaa aver DEN a SPh.
Caablaatioa iarreased hepatocellular carclaaaa aver DEN, SPD, * DOT.
Marked iacreaee ia hyperpleatic liver aodules.
Mat a praaatcr of akla luaore.
Little or aa alia tuaur iaitiatioa.
Aroclor IJi4
Aroclor 125* Aroclor 12S4 Aroclor I2SA
Skia
Dial Diet Diet
he laic
icfeie Before Defers
2,12-DNhA TPA
Skia
Walker 25* Inlretimer cel la MicrUr
USD cclle
Irlrieuacl(
Hulrney Irufccal* vlcufl
let fi'
COI aouae
Da diffcreace or decrease ia shia timer laducliaa aver 2,12-OHhA * TPA, dcpeadiai aa pretreatawal iaterval.
SpragueDawley ral
Timor growth iahihited. Survival liae iacreaecd.
C57AL/A sorer *
* mihocci growth of ttSh (ueo(.
Bslh/c house
Dif Mi effect oocogeoeele of Holoney
Del. tlahluro. ct at. 1914 Hafciura, et al. ID* Hlahlzual, 1929a,b Hlahizuai, I929a,b Miahizuai, IDla.h llo, et al. I92S
Derry, et al. I91S. 1929
Berry, et el. 1919
DiCiomal,
1917
Berry, el el. 1979 UiCiovinni, el l. 1977
Kcrkvliel ud KiwIJoll, |9/7. b
Kcrkvlict and Kuller, I92S
holler, 1922
QQfcSOO wC1V
WATER PCB-SD0000034171
1242 1221
Slat Diet
Tlaa
tuin-cMtUMi
Bi--tuiy or co-ca>c i hogemesis studies mitw pco
(Khar Trcataeat1
Species
Obaervalla
Delete Defers
Malaaef leukeala virus
Malaaef leukeala vlrua
lalrapcrlleacal
Delb/c aaaac
lalraperllaeeal
Dalk/c asuaa
111 aat affect sacageaeala of Malaaef leukeala vlrua.
DU aat affect eacegeacala af Malaaef leukeala vlrua.
Cheaicala usd; 20-HeCk - 20-aelhfIchalaalhreae a-IIIC = u-beuxeac hcaacblerlde P'lK * p-linitM keaacblarUa HI = dielkylallrasaalne All) = allalsala B, 2-l'AA = N-2-fluurenflacelaaUa J'-Me-DAS a I'-aelbfl-t-dlacIhplaaiaeaaabeaieae WIT = Maroilipkeafll r Itblataetkaac 111 - aoiliua pkeuobaihltal 7,12-lMA = 7,2-diaetbf lheax|a|aathraceae ,TTA - l2-0-lelr<lecaaoflpkarbal-l)-acctatc
1fre(uaat Haas Based with Kaacchler 400. Oflaprlag Based with DEM.
'Established tlsaue culture cell lisa derived fraa Malaaef aaresas vlrua-iaduced tuaata la 04 alee.
Del. Sailer, 1911 Sailer, 1971
AOM 0 0 5 9 6 9
WATER PCB-SD0000034172
AGM 0 0 5 9 7 0
l
Strain Fraduct AHOCIXMt I26S AMOCI-OM USA
AMOCUiH USA
AHUCIiMI USA Kanetlilor SOD* '
KnfitiUr 500
2,2\iii-telrchlorobi|tieMyt
CMH INU
Meg.
Meg
TAALt A fCNa; Tabulation at Microbial Ihtunilcltt Testa
CAS
TAM
TAIOO
TAI000
TAI51S
TAISIA
TAI51J- TAIS3S
ag.
Neg. Neg.
Neg.
Neg. Neg.
Neg.
Neg. Neg.
Neg.
Neg.
Neg. Neg.
Neg.
Neg. Neg.
Foa1
Neg. '
Neg.
clilofolii|*liriyl Kmililoi 300* Kincdilur 300 AMOCI.OM 1221
#
Neg* Mi**
Neg.
Neg.
#
Neg* Neg.
A -11 1 r ol> |Heuy 1
Meg.
Neg.
Neg. Neg.
Neg.
Neg.
1. All Irid done with and vllknit al.cn aaal actlvatloa eacegl oa anted,
2. Ollier rlrrlu tested, ace leal amd
e.
= Mol tested.
Neg. = Negative. Me** - Holy tested vltlioul activation.
Meg* " duly tested vitl actlvatloa.
Foa* = Tositivc only wltli activation. Fus^ - llnteitsin. See teat.
Neg.
Neg* Neg.
Neg.
Neg.
Foa* Fna * Neg.
fi i I
C. call
WF2
WF2 ovrA'
lei.
Neg.
* Neg.*
Neg.
VyndhMa et el. 19/6 Frobil. et el. 1911 Neddie end truce 19/Z Wyndbaa, et a 1. 1974 OdhiM, 19/6 Sugimrt, ei *1. 19/6
1 Vyndhaa, el *1. 19/6
# Neg.* Neg.
Neg.
lists, etal. 1911 Oilarltiu, 19/6 Su^iaurr, el !. 19/6 Wymlbse, cl *1. 19/6 Hyndliaa, el_al^ 197* Hdidltuii, cl el. 19/9
WATER PCB-SD0000034173