Document zzXaXDDMY0YjLkNOvKOZ1Vy3g

^^^..^^p AR226-2950 if^^E;3;?^?^?!1-.?!^^ :-- ''^":".' ,_..;, .; . ^''-? l"^-"';'1''^^'1.'^* . ;, ^,'.';%!.i";3-"f '^ ^i "^ ' ^ . ' -" r::--' py ^iT.^:i^^ ;^ I)u pont S"1 3^9-90 Study TitU STATIC ACUTE 48-HOUR EC50 OF DAPHNIA MAGNA Data Requirgment United State? Environmentfti Protection Agency Bnvironaontal Effects Test Guidelines Subdivision EG-1 Author Kevin N. Baer, Ph.D. Study Completed On 16 July 1990 Haskell Performing Laboratory i E. I. du Pont de Nemours and Company, Inc. Laboratory ior Toxicology and Industrial Medicine Elkton Road. Post Office Box 50 Newark, Delaware 19714 Laboratory Project ID i Haskell Laboratory Report No. 349-90 Page 1 of 13 onspany Sanitized. Does not contain TSCA CB8 Du Font HLR 349-90 'ATBffiNT This study vas conducted to U)(iId^St|i^es Environmental Protection Agency's Good Laboratory Regulati^fr <40 CFR 792) for TSCA. Areas of non-coapllance are docuoentejd in the^tudyirecords. No deviations existed that significantly affected |the validity of^fbe ^tudy. Sponsor: Submitter: E. I . du Font de Honours and Co., xnc. Chein|icals and PigBents Department Study Director: /^^k /U. ^LtiLA Kevtii N Baer, Ph.D. Rese arch Toxicologlst Environmental Toxicology and Information Services S.'J^e.^O Day/Mo/Yr Company SanRized. Does not contain TSCA CBI ^^f.:;..:-.;:-:;.^;A;:^-:;WfNI^s -{SitV-.V.V ^% h^S^ ; .' .^%1^'' Du Font BLR 349-90 ^'""||^:^ QUAIJ^YASSUB^g^E9(^EATION -UDY; H 18,267 STATIC ACUTE 48-HOUR EC50 OF| AUDITS: Items Audited Protocol, Conduct Records, Report Audit Dates 5/24/90 7/5-6/90 FTO DAPHNIA HAGNA J ^ l SHORT-TERM AUDIT REPORT NUMBER: DATE FINDINGS REPORTED TO MANAGEMENT AND STUDY DIRECTOR:-7/6/90 [ Reported by: l^^--^/ <s^^-- j Villlam J. Lynam Quality Assurance Auditor y//^.v Date 3 - - esUffss^ Sanitized. Does not contain TSCA CB1 "f^yfyw ./'^-,!:.;A" '.W'w"', ' .; .. W^"'^fSiK!: ^^ig, : "'^ ' : , ::y-..' - '-a;^s- TABHS^OF' '^GdlffiRBTS''{:' ~V'I^W i-"..-f '~fX\"i^~y'^-"~ ' '>':'' .:' yt1 "s?. Du Font BLR 349-90 Page Good Laboratory Practice St imnent 2 ................. Quality Assurance Docunenta :lon 3 ................... Table of Contents .j 4 ..................... .... I. Purpose 5 ...... ...... .1.............. II. RInetcroorddusction/Summary 55 .........I;....................................... General Information ..! 6 ..................... III. Materials and Methods 7 A. Test Material 7 .!..................... B. Dilution Water 7 ........................ C. Animal Culture 7 ....................... D. Test Methods 7 ....................... E. Chemical Analysis 8 ............................................. IV. Results and Discussion 8 ' ..................... V. Conclusion 8 ........................... VI. Literature Cited 8 .....l.f..|...|................................................................................. .... fB9 ......... VII. Tables Table 1. Table 2. Table 3. Table 4. Table 5. \. Chemical Characteristics of Haskell Laboratory Well Water 1. Water Chemistry of the Water Control at Test Start . Dissolved Oxygen Concentration of pH of Observedlmmo^ility of Daphnia magna Exposed to for 48 Hours in a Static, Unaerated Test 9 9 . 10 11 12 13 fS'.iYf-i.Wi^BPf, : '.^a Compai-iy .aa/Uized. uoes <;,,,( contain TSCA CBi s^ ,,^, <- -!-^^ ...^^ Du Font HLR 349-90 .w The objective vas to Wf^an the (icuiMs'^-^^etty aagna neonates less than 24 .. hours old." : "; of fl9 to Daphnia II. iiiiitopocrfon/; _____fexhibited ho acute toxleity t^ Pa^inia mapna neonates (less than 24 hours old) In an unaeratrd, static 48 hour ^st. Nominal concentrations were 1311, 1638, 2048, 2560 3206, and 5000Bg/L. The 48-hour EC50 could not be calculated because 5000 iig/L, the highest concentration tested, failed to cause immobilization of the daphnids. ! BEOOBDS All data and the finaljreport will be maintained either at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Delaware) or be archived at the Du Font Records Management Center (Vllnington, Delaware). ^ L ^ / L ^ Technician: Charles :-. Hall Environmrntal Toxicology and Informat -on Services T^r ^ 7 9o Day/Mo/Year M^^/u' ^L^ Study Director: _/[W^ /U' Kevin N.IBaer, Ph.D. Researchj Toxicologiat Snvis-onintental Toxicology and Information Services ^7 S)^c ^0 Day/Mo/Year THIS REPORT IS APPROVED ^OR ISSUE. K e,vfi n J ^ N. i iB ft/. aer , / ^ P RA h^. ^ Study Dik-ector /^ 3T/w 70 Day/Mo/Trear Company Sanitiz.ed. Does not contain TSCA CB1 , OO^100"^ San-^-00 GO,wpa^ III.! MATERIALS AND METHODS Du Font HLR 349-90 A. Test Material Source, composition and! purity ofH^^^^are described under General Information. The anpropriate quantity for each concentration was ot^Up weighed and dissolved in 1 liter of filtered fish tank water (see below) and and then distributed into four 0.25 liter containers in the water bath. Stability and solubility in dilution water were not determined. offBI ' B. Dilution Vbter Water (Table 1) originated from the Baskell Laboratory well and flowed through aquaria containing fathead minnows (Pimephales promelas) prior to use in these daphnid tests. C. t^sol Culture Parent Daphnia magna were reared at Baskell Laboratory in 2-L pyrex beakers (21 per beaker) which contained 1.8 L of filtered dilution vater held at approximately 20C. Daphnids were fed 30 ng/L (dry weight) of a mixture of frozen Purina (same as Buckshire) trout chow and Fleischnann's yeast three times weekly during ttie water renewal process. Neonates were collected from 15 day old parent daphnids for use in these tests. Sickness, injury or abnormality was not present. Animals were identified by labels on the beakers or test vessels. D. Test Methods [1] Six test concentrations and a water control were prepared using filtered fish tank water. Nominal test concentrations were 1311, 1638, 2048, 2560, 3200 and 5000 mg/L. Pyrex beakers (250 roL; 6.1-cm diameter) containing 200 mL of test solution (6.8-cm depth) were used. Each replicate (four per concentration) contained five daphnids (<0.1 g daphnld/L). Daphnids were added to the water control and to test beakers beginning with the lowest concentration. Random assignment was not used to avoid contamination of test solutions of low concentration from the transfer pipette when daphnids were released under the water surface. Daphnids were not fed during the test. Test solutions were held between 19.9 and 20.1C (mean IQ.O^C) and were unaerated. A photoperiod of 16 hours light (506 to 592 Lux) versus 8 hours darkness was employed. ,,.,.------- W?SSSt^S:y^ ^^?y| Dissolved oxygen replicates of every f^il^c8"^ beginning and at the ^emt^f 'jfl conductivity of the dilution?!! Tenperatures were taken yith'-i calibrated mercury therapDeterj B. Chexical analysis No chemical analyses of Ou Font HLR 349-90 iter controls and in all lids vere added at the iity, EDTA hardness and the beginning of tlie teat* Testing Materials test solutions yejce perforBed. . RESULTS AND DJSCVSSION An earlier rangefinding reported in this study. vas conducted but the results will not be TT.ns solutions of fl^----1exhibited a Bllky white color. All chemical and physical parameters (Tables 2, 3 and 4) were within expected ranges. The 48-Hr EC50 could not be calculated because 5000 mg/L, the highest concentration tested, failed to cause iBBiobilizatlon of the daphnia (Table 5). V. COSCLUSIOH 'exhibited no acute toxiclty in this static, unaerated 48-hour Daphnfa magna test. The EC50 u&s greater than 5000 og/L, the highest concentration tested. VI. LITERATURE CITED 1. United States EnvlrpnBental Protection Agency, Office of Toxic Substances, Guidelines for Tes (ting Chemicals, EG-1, Washington, DC., August 1982. Company Sanitized. Does not contain TSCA CBi Du Font HLR 349-90 TABLE 1 Chemical Character! jstics of Bsskell Laboratory Veil Vateri Paraaeter Concentration Paraaeter Concentration BOD, mg/L COD, mg/L DOC, og/L TOC2, Bg/L Kjeldahl N, mg/L Aomonia N, og/L Organic N, mg/L T--bldifcy, MTU Phenolics, mg/L Color, Co/Pt Solids total suspended, Aluminum, mg/L Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Boron, mg/L Cadmium, mg/L Calcium, og/L .Chloride3, mg/L Chromium, mg/L Cobalt, mg/L Copper, mg/L Cyanide, mg/L Iron3, mg/L mg/L 5 <10 <lr0 0.8 <0.2 <0j,2 <0,2 <lg.O <0.05 <5 <4 <0,100 <0,060 <0.010 <0.001 <0,021 <0p002 25,4 6.3 <0.010 <0.020 <0,010 <0.025 0.131 Pluorlde', mg/L Lead, mg/L Magnesium, &. 'L MBAS/LAS, mg/L Mercury, mg/L Hickel, mg/L Nitrite3, mg/L Nitrate3, rog/L Phosphate3, fflg/L Potassium, fflg/L Selenium, mg/L Silver, ng/L Sodium, mg/L Sulfate3, mg/L Sulfide, mg/L Zinc, mg/L Volatile priority pollutants Acid extractuble priority pollutants Base/neutral priority pollutants Pestlcides/PCBs Organophospha t e pesticides^, ug/L <0.l <0.075 3.9 <0.05 <0.00020 <0.020 <0.1 1.7 <0.1 1.8 <0.005 <0.010 8.1 5.0 0.11 <0.020 MDS HD6 ND6 MD< <0.50 lDate of analyses 24 October 1989 unless Indicated otherwise. 2 Date of analyses 5 December 1989. 3 Date of analyses 11 December 1989. ^Date of analyses 26 September 1989. SNone detected except methylene chloride vas belov the published Method Detection Limit (BMDL). None detected. noic^8'"^5'CP^ >niied. Does CotW^58" TABLt ;?: Water Chenist]ry of the Water Control at Test Start Total Alkalinity (ng/L as CaCO,) EDTA flardness* (Bg/fc as CaCOia) COt.ductivity1' (Vinhos/cm) 76 77 162 'Bach kit. Bach Chealcil Company, Loveland, CO. ^SI Model 33, Yellow Springs Instruments, Yellow Springs, OH. 10 ^.-.OO..------------"081 ComP^ sa ^i V. <" t E(, !-.. 1:i-. f 1' l-i ?'- | | | | - ^wywyw^ '.'' ' '. . ' ' ' Dissolved Oxygen i ---------------------------- i, i '^^^^'^^^ ''. '.. ..;;u^?:^]^i .'^^;;:;j!il . .. '-. .,:. ' ..... ^..,, - ; -.--'.:."'.-.'"- "-';"--^a-s'a1".t--iT'--^S-'1^^) '.... ,;.;., .. ^.tfe'.T..::^ -. . ',.. '-"^:-:"?@ ,,. ;"- -, ': :. -/?-, ^^-^'^^:^v^:!^^ -'.<; ' '; '' Du Font HIA 34^-90 f | yTest ^^^v^^'"1''^^-' ' -t;^ " ' ';'r-yt '- '^ -tV- i-^;'i.)urrft oncentrfttlbhsi^ of ' .: .>3.. . ^.C'i Solutions " ~ . '.";E-.:. ^- ^a^i^. . v'^3lJO. xygen2 (ng/L) ^ ? % ^ .,,, " "::; "".^ K8l| ' ..'.''-.a ".''M " '^. .- . ''M " ^ : y: . f y Nominal Concentration (Bg/L) 0 Hours i 48 Bours '^ ' " '"-' A2 B C D A- B' c' y ^'i i Water Control 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 3 1311 ! 8.8 0u 0*0 0*0 8.8 8.8 8.8 8.8 - . . i ', 1638 8.8 8.8 8.8 8.8 B B 0 o 6 o oo i 2048 ^. 8.8 8.8 8.8 8.8 8 8 Bo oo 0*0 2560 3200 8.8 0*0 0*0 O-B 0 8.8 8.8 8.8 8.8 8.8 | 8.8 8.8 8.8 8.8 8.8 8.8 8.8 ' 5000 S.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 ^SI Model 33, Yellow SpjringsINstruments, Yellow Springs, OH. 2 Five daphnids in each rjeplicate. : 1 t 11 . o ^ ^ ^paffii ^^ooes GO' ^^^^^^^^^^^B|B|Kg .''-.' ^^1^1 "' ' '-'^'''.'.sS'--:;^ .. / ^^ ^a^if"? ' ^'' ^^^M ::, .': D" Po"t Btt 3*9-90 '''' ' ' ^ i l l "' .^W^ 1 pr --------- 1 .------:.'!^L^:K.L:'i.J^----------"" --""'' !------ lll.'IIN,'. .. . j ^ :^ '' : pH1 '' Z ' . Nominal Concentration (Bg/L) 0 Hours - 48 Bours ,3 ? I ).-. -------------------------------------------------- 3 .---------------^-.----..j.Y m!-;., - A' | B2 C? D2 A2 B C2 D2 ' Vater Control ! . 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 lall 1638 2048 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 \ fe 2560 --/ 3200 i 7.7 7.7 7.7 7.7 7.7 7.7- 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 5000 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 corning Model 610A, C<irning Scientific Instruments, Medfield, HA. 2 Each replicate contali ied 5 daphnids. 1 i ; 12 i i ^ I c^s----------"01""1-"""06' i 3 ^oHIUy j. 1HB-8-5 :'; : .". 1^ ObservedfoInrm4o8bHilol uyrsolfnP'5a-pSh?nSia?lacgf-ntaeeferyaptoesdedlst ; ' 1 ' ' ' : 3'. ii" -......---------------------------. . "---------------- ; ^ercent Cumulative Observed Immobility ?- 1' i- S4 Hours 48 Hours ?' Nominal r. i , , ,, ,,'; |< Concentration (mg/L) Ai JBI C* D1 A1 BI C^ D1 ; - i ^- f Vater Control 0 0 0 0 1311 0 0 0 0000 OjO ' 0 0 0 ' 1638 0000 OJOOO f 2048 : 0!000 0000 1 2560 ! j ':: o!o00 0000 ; 3200 0000 0000 j i]: i : 5000 0,000 0000 1 * {', --------------------------------------1------------------------------------------------------------------------ ^ach replicate contairi ed five daphnids. i i ! 13 ! i^ ^^.00---------------------- j