Document zzNMBmrk9Qemjn63wNYVaXzY7

R a .6 _ 0 7 5 5 MICROBIOS M ICROTOX TO XIC ITY TEST TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 519. The test sample is FC203CE. Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: Microbios Microtox Procedure Test type: Acute static GLP: No Y ear Com pleted: 1989 Species: Photobacterium phosphoreum Analytical m onitoring: pH and osmotic condition. Exposure period: 30-minutes S tatistical m ethods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbios' Reagent. Test conditions: Diluent: Microbios 2% NaCI solution Test tem perature: Not noted. Stock and test solution preparation: A primary 2000 mg/L stock solution was prepared in Millipore Milli-QTM water. The pH of the stock solution was 6.8 and an osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "completely miscible, no color apparent". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Num ber o f replicates: two Num ber o f concentrations: Four plus a blank control Elem ent basis: Percent light loss RESULTS____________________________:____________________________ Nom inal concentrations: Blank control, 125, 250, 500, and 1000 mg/L 001116 Elem ent value: 5-minute EC50 - 370 (360 - 380) mg/L 15-minute EC50 = 260 (250 - 270) mg/L 30-minute EC50 = 230 (220 - 240) mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test substance 30-minute EC50 for Photobacterium phosphoreum was determined to be 230 mg/L with a 95% confidence interval of 220 to 240 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G 1358-1,1989. OTHER___________________________________________________________ Last changed: 6/26/00. 001117 ENVIRONMENTAL LABORATORY MICROBIOS MICROTOXR SYSTEM RAW DATA '<> jZ 5 i~ - I TEST SUBSTANPF FC. ~ & 0 3 C& TEST DATE ANALYST: <7 - & a rL 5 ! CI f SAMPLE CUVETTE .r "INITIAL --- -- PINAT'READINGS FINAL ASSAY CONCENTRATION tvya ll* READING 1(0) 5-MIN. 1(5) 15-MIN. 1(15) 30-MIN. 1130) G B1 BLANK CONTROL 4 4 2 o /y 70 Cl DUPLICATE 9 6 ~ / 71 70 ' B2 )7 S S> s r 45" 41 C2 *Dup)icL4. 9 to n H -S B3 A so 'ML V 6 37 33 C3 i x > plcA-ie 4 7 *n 33" B4 TOO 5 7 3 A . 5 ~ 2 1 C4 X>up) iYa4c 93 3H * j AA B5 / o o o 9 Y /? 1 5 " C5 T>upliCale. 96 AS 17 iH Tesrf yeiiccc Uidoic^ Smoke- +-<:k 5*?)affon g ;7o> mj //o/*i_ /Ik | - d OJtf4r- itx c io ).o r>dL ~ .D yyJhrJ Apparehty fm&r'fc>le A/o C>(or a tfAtflmf" s + c fc S a n - pA f= ^ ' V * a o v ^ A J< id l+ o i o StocV So|uf/oh l!> 11 o e r\+ ^ i'K c x i. ^ ^ IO A S : M ic ro b e s % JJdLcJL S o l o f lo n In iv ia A O-'oncetnrVtfcHonS, wOe. cXoobU. +W. C it\<dL O&ty Q0ncttoa4ioh nertkorwA <^oc*je."tVi. i m t a ! <X\. [w hons mere. jn4rocUoec /K o 4 k c . fop Ckcm^A) cvni. <-oer- 4V^ve +Rms.re<fi. t/sc>\ iM o -fk e hvi d a i - jruU-Ua- 0LOr rocus C f io ^ 8 ^ 0 0 0 1 1 1S s-'i-v. 'X":. MICROTQX(r) DATA SHEET BEST COPY AVAILABLE LR Gl358-1 FC-203CE 5--MIN EXPOSURE 6/16/89 SAB PAIR # 1 2 T 4 5 6 7 8 . CONO. JEUSlI * 125.000 125.000 250.000 250.000 500.000 : 500.000 7.1000.000 7.1000.000 , Io/It 8S.0/ 55.0 96.0/ 59.0 92.0/ 46.0 97.0/ 49.0 87.0/ 32.0 93.0/ 34.0 94.0/24.0 96.0/ 23.0 G-BS 0.364 0.387 0.705 0.6B 8 1.318 1.332 2.339 2.559 G-EST 0.375 : 0.375 0.702 0.702 1.313 1.313 ,2.456 2.456 IVrcerrvvVV tIi1A3V.Vft -A--------rr~ HI HI 3577 70 72 BLANK Bo/Bt* 95 / 81 BLANK R A T IQ* 0.8526 l ^,T` ;, -i : EC 50 = 3 7 0 .001 ( 358.387 TO 381.992 ) ' R =0 . 9 9 9 1 5 SLOPE = 1.1045 INTERCEPT * 3 ?o (3 i o - 3 t ) y ^ l L +5 .9 1 3 5 1.* /'}{:' if} ^ . - 1 L, : -'`.W-h v ## 1 --,r 4 c; w 6 7 :& RES. PRDB. 0.054 98 0 .0 5 2 90 x 0 .0 3 5 40 0 .0 3 5 41 0 .0 3 5 41 0 .035 41 0 .051 88 0 .0 5 5 100 i ', I'. ,{ X', . , ; ; .* . . ! i : : ! : 1> i \ ! i 1 0> ' ' 1 i.V:' ,' ;, ' ; \ .1 :0 . . x < 'G.'X -8- t .--- 7 . 1 6 ' '''!V "-l ;--- p ; " -5 , "* W mm ^ i ' > ' :-l'K H f;!: :< v s > ; 11 -4- LLIST C FF :E 2RUN 0N K N T E -1 +4 L a Y D LOG CONO. 4SA 7TR 0S E 0N C 8 R P o o iiis te LR 61358-1 FC-Z03CE MICRGTOX<r> DATA SHEET 15-MIN EXPOSURE 6/16/89 SAB ;>.l -f<if n l PAIR # CONC. Ia /It B-QBS G-EST : P * Ik n1j 125.000 125.000 88.0/ 45.0 96.0/ 49.0 250.000 92.0/ 37.0 0.523 0.526 0. 937 0.519 0.519 0.953 w ss M ||l | |1 j ijy ^ j [ i||iii- <.; ,:& 9 34 : M ;; ^a 2 y-* ; K 4 250.000 97.0/ 39.0 0.937 0.953 ; 5 500.000 87.0/ 25.0 6 500.000 93.0/ 26.0 1.711 1.786 1.749 1.749 ; i; ir3 : /ii. 5, ft: . l: : 7 /.1000.000' ' 94.0/ 18.0 3.068 3.210 : '7 s r^ 7 - V - ' 8 7.1000.000 96.0/ 17.0 3 . 3 9 9i 3.210 u . j jM!-1! BLANK Ba/Bt* 95 7 74 BLANK RATIO 0.7789 ( 0 G*obs j ij'V jJV "vivrl'T-J,7\ Si EC 50 = 264.385 ( 254.982 T O 2 7 4 . 1 3 4 > 4 U> ( 2. S o - A 7 o } * , !;i r' w R =0.99904 SLOPE = 1.1388 INTERCEPT = +5.5774 ## RES. PROB. 1 0.056 o 0.056 T 0.037 91 91, 40 ' 4 0. 037 40 5 0.037 39 f1 1 1 ! 11\ 6 0.038 7 0.055 8 0.059 41 1- >. 87 lOO '[ 1 0 -V- h_, J' " -4 -. *:..-. : ' --- ! 1 . . 11 i 1, V 1 L 8-1 a ;,(3 -' I :G 'in ;, l i: 'BI- M lT .1ST C F e 2RUN 0N K N T E 1 +4 LOAD" , "LPT Y \ I 5 LOG CONO. 6; 7 4SAVE " V5 f 7TR 0N 8 TR 0 o s c r .e~~ ;:.le..i' s, -;-i LR G 1358-1 FC-203CE M I C R Q T Q X ir) D A T A SHEET 30--MIN EXPOSURE 6/16/89 SAB PAIR # CONC. 1 i '".y 4 5 6 ::7 ;U 125.000 [ 125.000 250.000 250.000 - 500.000 500.000 7.1000.000 XI000.ooo Io/It BB.0/ 41.0 96.0/ 45.0 92.0/ 33.0 97.0/ 35,0 67.0/ 21,0 93.0/ 22.0 94.0/ 15,0 0/14.0 G-OBS G-EST 0.582 0.572 1.054. 1.042 2.053 2. 115 3.618 4.0B3 : ; 0.570 0.570 1.078 1.078 2.037 2.037 3.85 - , 3-.8S I<;r T&r'.';* f^rccn+ Llgtit 37 T?T?T|F- 3b 5*1 S\ u 1% `ViT-U i t-ii; 001121