Document zzJqk93vDNpY0w92bgKM13rbR

Ak 2.6 - Q Z $ 0 ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE____________________________________________ Identity: A mixture containing periluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600 (SCAS Treated Effluent*). Current information indicates FC-600 is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3~ chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentration o f impurities that was treated in a SCAS reactor. Data may not accurately reflect the toxicity of die fluorochemical component o f the test sample. This study is the third of 3 daphnia studies conducted to determine the toxicity of FC-600 after being treated through a SCAS reactor. This study is the control for the FC-600 SCAS Treated Effluent study. It used the supernatant of a mixed microbial culture that initially contained no FC-600. The effluent was collected after 4 hours of treatment in a SCAS reactor. The inoculum used came from the Metro Wastewater Treatment Plant, St. Paul, MN. METHOD:____________________________________________________ M ethod: Not noted. Type: Static acute GLP: No Year com pleted: 1977 Species: Daphnia magna Supplier: Not noted. A nalytical m onitoring: Dissolved oxygen Exposure period: 48-hours Test organism age: Not noted. S tatistical m ethod: EC50 calculated by graphing data and by probit analysis. Test conditions: D ilution w ater: Carbon-filtered well water, aerated. D ilution w ater chem istry: Dissolved oxygen: 9.4 mg/L Lighting: Not noted. 002012 Stock and test solution preparation: Volume/volume direct addition to test vessels. Exposure vessels: Not noted. Number of replicates: two Number of daphnids per replicate: 10 Number of concentrations: six Water chemistry during the study: Dissolved oxygen (final): 7.5 mg/L (5.6% exposure) 6.5 mg/L (100% exposure) RESULTS_______________________________________________ Nominal concentrations: 5.6%, 10%, 18%, 32%, 56%, and 100% Element values: 48-hour EC50 =15 (13 - 18) % Element values based on nominal concentrations Remarks: Testing was conducted on the supernatant created from a SCAS study as described in the Test Substance Remarks field. The values reported apply to that solution and not the test substance. CONCLUSIONS__________________________________________ The SCAS treated effluent 48-hour EC50 was determined to be 15% with a 95% confidence interval of 13 to 18%. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ Reliability: Klimisch ranking = 3. This study lacks description of the methodology used, reporting of test conditions, and information on characteristics of the effluent after SCAS treatment. REFERENCES_______________________________________________ This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, 1977. OTHER_________________________ ___________________________ Last changed: 6/28/00 002013 ,740101 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET / - ^ - Type Test L(% 98 Hours -- TaiegmPcc stmy / \ r' pAfROiA MA*OA Test Organism Eathead Minnow fC. too Material Tested SC-/1S C O N T R O L ( s u P F C M Avg. Weight ___________ Size Date Started_____$ / # / ? ? Time Started_____/ ; ? o //yy. Date Completed Plotted LCcn 9hom Dilution Water Carbon Filtered - Well Water fce fcfcrc t> t o BEST COPY AVAILABLE 002015