Document zzDn7JLbV70GjZQ5jmrdXV2Kg
AR *6 -
TO XICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-3017. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-3017. Current information indicates FC-3017 is a mixture of 0.67% PFOS, 20% diethylene glycol butyl ether, 55.41% water, 21% ethylene glycol, 1.33% Hydroxy foamer, 1% sodium octyl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyltraizole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity o f the fluorochemical component o f the test sample.
METHOD:________________________________________________
Method: OECD Guideline 201 and EPA CFR 40 797.1050. Type: Acute Static GLP: No Year study performed: 1996 Species: Selenastmm capricomutum Source: In-house cultures. Originally stock from ATCC, Rockville, MD Element basis: Algal cell counts (cells/ml), specific growth rates. Exposure period: 96 hours Statistical Methods: Not applicable due to study results. Analytical monitoring: Not given.
Test Conditions:
Algal Nutrient Medium: Nutrient medium for freshwater algae per OECD method. The pH of this medium was 7.9. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by diluting 500 mg of test substance in 500 mL of standard algal nutrient medium. Aliquots were then added directly to dilution water in test vessels to create test solutions. Exposure vessels: Not given Agitation: Not given Number of replicates: Not given Initial cell loading: 1 x 104 cells/mL Number of concentrations: one plus a blank control Lighting: Continuous Water Chemistry: Not given.
RESULTS________________________________________________________
00282
Nom inal concentrations: Blank control and 1000 mg/L
A lgal Growth Response ErCso values
Exposure (C o n ta c t)
Hours 24 48 72 96
Mean Percent Change in Specific Growth Rate (1000 mg/L Cone.) 15 9.1 16 17
E,C50 (m g/L)
>1000 >1000 >1000 >1000
Element values are based on nominal concentrations.
Rem arks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C L U S IO N S ___________________________________________________
FC-3017 exhibits a 96-hour ErCso for specific growth rate of >1000 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking = 4. Not all laboratory data could be located. Study lacks description and records of the method followed. Sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________________________________
This test was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number N3188, 1996.
O T H E R _____________________________________________________
Last changed: 6/26/00
002826
05/30/2000
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
1
LAB REQUEST NO. N3188 REQUESTOR NAME: SBS/BOB COX DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 09/20/1995 DESC: FC-3017 (LIGHT WATER)
CONTRACT LAB(S): ASCI EXP COMP DATE: 11/22/1995 DATE COMPLETED: 05/23/1996 PROJECT LEAD: SA BEACH PHONE NO: 651-778-7452 3M FAX NO: 651-778-6176
Standard set of tests. Please make this a priority project as Europe needs data as quickly as possible without incurring additional costs. Please also copy Agnes Vidal and Rene Lehenaff on testing results. MSDS Doc.# 10-3922-1.
BOD and COD will be put on another lab request. sab 9/28/95
Results from Microtox Test:
Test Cone.
Percent Light Loss
mg/1
5 -min exp.
15-min exp.
125 6.5 9.5
250 16 22
500 26 37
1000
44
57
EC50
> 1000
780
95% Cl sab 9/28/95
(-)
(730 - 850)
30-min exp. 14 " 28 46 67
560 (540 - 570)
Results from 96- hr Fathead Minnow Toxicity Test:
Test was run as a static-renewal. Beakers were renewed at 48 and 72-hrs
of exposure. This was done because the dissolved oxygen levels dropped
below the required 60% of saturation. (1000 mg/L renewed at both times,
100 mg/L renewed only at 72 hours, sab 10/27/95
Test
Percent Mortality
Cone., mg/L
24-hrs exp. 48-hrs exp. 72- hrs exp. 96-hrs exp.
1.0 0 0 0 0
10 0 10 10 10
100 0 0 0 0
1000
0000
Duplicate 1000
0
0
0
0
LC50
>1000
>1000
>1000
>1000
NOTE: The blank controls and 1000 mg/L concentrations were duplicated; this test combined a range finder with a definitive test at 1000 mg/L. sab 10/27/95
Results from Activated Sludge Respiration Inhibition Test:
Test Cone., mg/L
1000
Dup. 1000 EC50
Percent Inhibition in Respiration Rate
30-min Exp.
3-hr Exp.
00
00
> 1000
> 1000
No significant inhibition in respiration rate was seen after 30-minutes and 3-hours of exposure to 1000 mg/L.
sab 10/17/95
002827
BOD- AND COD ARE ON LR N3688. sab 11/27/95
Results from algae toxicity test:
Test Cone. mg/L 1000
Mean Percent change in Specific Growth Rate
24-hrs
48-hrs
72-hrs
96- hrs
15 9.1
16 17
EC50 sab 1/16/96
>1000
>1000
>1000
>1000
Results from Daphnia magna acute toxicity test:
Test Cone. mg/L
1000 (all 4 reps)
Percent Immobilization
24-hrs Exp. 0
48- hrs Exp. 0
EC50 sab 5/22/96
>1000
>1000
-
SAMPLE DATE
CODE
DESCRIPTION RESULT
* = CONTRACT LAB DATA
05/30/2000
LAB REQUEST NO. N3188
SAMPLE DATE
CODE
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
CONTINUED
DESCRIPTION RESULT
1
FC-3017
Amber Liquid
ASCI
48-HR-EC50 *DAPHNIA ACUTE TEST 96-HR-LC50 FISH ACUTE TEST - 96 HOUR IC50-ALGAE ALGAE ASSAY - IC50 MICROTOX MICROTOX - EC50 SLUDG-RESP SLUDGE RESPIRATION - EC50
>1000 MG/L >1000 MG/L >1000 MG/L 560 MG/L >1000 MG/L
MDL or (95% C.I.)
2
MDL or (95% C.I.)
(-) (-) (540 - 570) (-)
002828
BEST COPY AVAILABLE
ENVIRONMENTAL LABORATORY
PREPARATION WORKSHEET FOR FRESHWATER ALGA GROWTH INHIBITION TE S T"
LR SEE BELOW
PAGE OF DATE 10 M M s
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002829
BEST COPY AVAILABLE
NUTRIENT
ENVIRONMENTAL LABORATORY STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE
CONCENTRATION IN STOCK SOLUTION
FINAL CONCENTRATION IN TEST SOLUTION
LR M1655 N31B8 N3194 N3281
STOCK SOLUTION 1: MACRONUTRIENTS
NH4CI MgCI* 6H20 CaClj 2H20 MgSO 7H20 KH2P04
1.5 gA. 1.2 gA. 1.8 gA. 1.5 gA. 0.16 gA.
15 mgA. 12 mgA. 18 mgA. 15 mgA. 1.6 mgA.
STOCK SOLUTION 2: Fe-EDTA
FeClj 6HjO Na2EDTA 2H2O
80 mgA. 100 mgA.
80 ugA. 100 ugA.
STOCK SOLUTION 3: TRACE ELEMENTS
HjBOj
MnCI* 4H20 ZnClj CoCI* 6H*0 CuC^ 2HjO NajMo04' 2H20
STOCK SOLUTION 4: NaHC03
185 mgA. 415 mgA.
3 mgA. 1.5 mgA. 0.01 mgA. 7 mgA.
'V *
185 ugA. 415 ugA.
3 ugA. 1.5 ugA. 0.01 ugA. 7 ugA.
.k v.
NaHCOj
50 gA.
50 mgA.
~
Sterilize stock solutions 1-4 by membrane filtration {0.2 urn).
002830
BEST COPY AVAILABLE
002831
ENVIRONMENTAL LABORATORY
FRESHWATER ALGA GROWTH INHIBITION TE S fU SPECIFIC GROWTH RATE m
ALGAL SPECIES: Freshwater Green Algae, Selenastrum caprlcom utum ^^
DATE INITIATED: | r t / a f o *
DATE TERMINATED: IO /|- * t o r t hrift
;
^
TEST SUBSTANCE CONCENTRATION mg/L
24 HRS
48 HRS
72
MEAN OR RATE % CHANGE" MEAN OR RATE % CHANGE" MEAN GR RATE
BK CONTROL STD NUTRIENT
Z.\<A
l.0>48
--- Jti 1,4*1*1
M l 655 1000 mg/L
I.R68
5.5
1.1.92/ tZ.
I.IVU
N3188 1000 mg/L
15 1.4R8
\.ZZG
N3194 1000 mg/L
0.55
15
O .S R T
I 4
aseo
N3281 1000 mg/L
1.841
15
1.411
14
1.155
DATE/ ANALYST
IQ/lftftK
Test methodology conforms with the blowing two test protocols^
Miia
'
OECO #201 *Atge, Growth Inhibition Tesr and Fed Reg. Vol 40, No. 797.1050, "Algal Acute Toxicity Test*.
..... ... ^ .
Speafie Growth Rate (u) InN .Infyt. Where N, is the mean measured final cel density; N, Is the Inittat cell density and t Is the lime of the measurement.':-^.:
' Percent Change in Growth Rate % (Mean Control Value Moan Sample ValueyMean Control Value * 100.
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